Method for clinically and rapidly detecting biological membranization bacteria on nasal mucosa

Abstract

The invention discloses a method for clinically and rapidly detecting biological membranization bacteria on nasal mucosa. The method comprises the following specific steps of: (1) preparing a reagent; (2) taking a target nasal mucosa sample, which is 0.3*0.3 cm, under a nasal endoscope; (3) putting a nasal mucosa tissue into a 2 ml epoxy resin (EP) tube, adding 0.5 to 1.5 ml of physiological saline into the tube and vibrating for 5 to 10 minutes; (4) dripping suspension on a slide, adding alcian blue on the slide, uniformly mixing the suspension and the alcian blue, standing for 3 to 5 minutes and heating and fixing with flame until dye liquor is fully volatilized; and (5) adding Congo red for uniform coating and covering, heating with the flame until the Congo red becomes dry, flushing with tri-distilled water, absorbing water, observing under an oil immersion lens and performing negative control on the physiological saline. In the method, a detection result is compared with a scanning electron microscope result and 100 percent of results are accordant. The method has the advantages of simple operation, low cost and extremely convenient clinical popularization in rhinology.

Description

technical field [0001] The invention relates to a detection method of biofilm bacteria, in particular to a method for clinical rapid detection of biofilm bacteria on nasal mucosa. Background technique [0002] The existence of biofilm bacteria is the main reason why most chronic inflammations are persistent and difficult to heal. There are also biofilm bacteria on the nasal mucosa of chronic sinusitis, which may also be the reason why it is difficult to cure. The current methods for detecting bacterial biofilms include scanning electron microscopy and confocal laser immunofluorescence (CLSM), and there are also attempts to detect polysaccharides on bacterial biofilms to determine the presence of biofilm-forming bacteria. Scanning electron microscopy and CLSM methods can directly detect the presence and shape of bacterial biofilms on tissue surfaces in situ, but they are expensive and take a long time to detect. At the same time, the cost of instruments and equipment required...

AI Technical Summary

Problems solved by technology

At present, the detection of biofilm bacteria is expensive, the detection time is long, and the required equipment is large and expensive, which cannot be widely used clinically.

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