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Method for rapidly obtaining pure line of hybrid wheat

A fast technology for hybrid wheat, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low frequency of microspore embryo induction, restricting the development and application of haploid breeding technology, etc.

Inactive Publication Date: 2013-11-13
甘肃省农业科学院生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For a long time, anther culture has been one of the main haploid breeding techniques. However, due to the existence of the anther wall, this method has the disadvantages of low frequency of microspore embryo induction and genotype has a decisive influence on the formation of microspore embryos. Restricted the development and application of haploid breeding technology

Method used

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  • Method for rapidly obtaining pure line of hybrid wheat

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 A kind of method of fast obtaining pure line of hybrid wheat comprises the following steps:

[0050] ⑴Preparation of hybrid combination:

[0051] According to the breeding goal, select varieties with complementary characters as parents, artificially emasculate and pollinate, after maturity, harvest hybrid ears according to the hybrid combination, thresh separately, obtain hybrid combination material seeds, and save them for sowing in the next year.

[0052] ⑵ Planting hybrid materials:

[0053] During the wheat sowing period, 60 seeds of the hybrid combination material were sown on-demand in the field with a row spacing of 20 cm and a plant spacing of 10 cm. After sowing, the ground was leveled with a rake along the sowing ditch. Field management should pay attention to pest control and disease control to ensure the robust growth of hybrid composite materials.

[0054] (3) Determine the appropriate ear-picking period:

[0055] Take the anther in the midd...

Embodiment 2

[0095] Embodiment 2 A kind of method of fast obtaining pure line of hybrid wheat comprises the following steps:

[0096] ⑴Prepare the hybrid combination; ⑵Plant the hybrid material; ⑶Determine the appropriate ear-picking period is the same as in Example 1.

[0097] ⑷ Pretreatment of anthers:

[0098] First with the mass concentration of 75% alcohol, the ears of wheat obtained in step (3) are surface sterilized and then put on the aseptic ultra-clean workbench, after the alcohol on the surface evaporates, the ears of wheat are peeled off; then the ears of wheat are put Put it into a sterile Erlenmeyer flask, add HgCl solution with a mass concentration of 0.1%, and shake it continuously for 4 minutes, pour off the HgCl solution, wash the wheat ears with sterile distilled water three times, take out the wheat ears, and dry the surface moisture with sterile filter paper , take out the anthers with sterile tweezers; finally inoculate the anthers in a triangular flask containing 3m...

Embodiment 3

[0116] Embodiment 3 A method for rapidly obtaining pure lines of hybrid wheat comprises the following steps:

[0117] ⑴Prepare the hybrid combination; ⑵Plant the hybrid material; ⑶Determine the appropriate ear-picking period is the same as in Example 1.

[0118] ⑷ Pretreatment of anthers:

[0119] First with the mass concentration of 75% alcohol, the ears of wheat obtained in step (3) are surface sterilized and then put on the aseptic ultra-clean workbench, after the alcohol on the surface evaporates, the ears of wheat are peeled off; then the ears of wheat are put Put it into a sterile Erlenmeyer flask, add HgCl solution with a mass concentration of 0.1%, and shake it continuously for 5 minutes, pour off the HgCl solution, wash the wheat ears with sterile distilled water three times, take out the wheat ears, and dry the surface moisture with sterile filter paper , take out the anthers with sterile tweezers; finally inoculate the anthers in a triangular flask containing 3ml o...

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Abstract

The invention relates to a method for rapidly obtaining a pure line of hybrid wheat, comprising the following steps: (1) preparing a hybridized combination; (2) planting a hybrid material; (3) determining an appropriate ear fetching period; (4) pre-treating anther; (5) dissociating and purifying microspores: (6) adjusting the density of the microspores; (7) forming microspore embryos by induction; (8) forming regenerated plants by differentiation culture; (9) allowing regenerated seedlings to pass the summer at low temperature; (10) culturing the strong seedlings; (11) hardening the seedlings; (12) transplanting the seedlings; (13) managing the transplanted seedlings; and (14) harvesting after the seedlings become mature. In the method, a test material is directly planted in a field and the microspores are dissociated from the anther for culture to eliminate the disturbance of an anther wall, thus being beneficial to optimization of various culture factors, improving the induction frequency of the microspore embryos, overcoming limitation on a genotype, promoting more hybrid combined materials to obtain the regenerated plants, providing more abundant base materials for variety breeding, and playing an important role in enhancing the breeding efficiency of a haploid.

Description

technical field [0001] The invention relates to a wheat breeding method, in particular to a method for rapidly obtaining a pure line of hybrid wheat. Background technique [0002] Hybrid breeding is a commonly used method for cultivating wheat varieties at present. This method is to plant the hybrid combination in the field and continue selfing for multiple generations. In the case of one generation per year, the hybrid combination between varieties generally takes 5-6 years time (distant hybrid combinations need more years), which requires a large number of materials to be planted in the field for many years, occupying a large amount of arable land, and only after a large number of identification and screening by breeding researchers can a pure line be obtained for further identification and screening. In addition, in the segregation generation population materials, because the alleles are heterozygous, only the traits of the dominant gene are expressed, while the traits o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/02A01H4/00A01G22/10
Inventor 杨随庄王纬叶春雷罗俊杰王红梅张艳萍厚毅清石有太谢志军陈玉梁
Owner 甘肃省农业科学院生物技术研究所
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