Kit for detecting hepatitis B virus (HBV) cccDNA (Covalently Closed Circular DNA) of paraffin embedded hepatic tissue

A hepatitis B virus, paraffin-embedded technology, which is used in the determination/inspection of microorganisms, fluorescence/phosphorescence, biochemical equipment and methods, etc. Complete and complete digestion of non-closed circular DNA to achieve the effect of large amount of detection, low pollution and high sensitivity
CN101948933AInactive Publication Date: 2011-01-19302 MILITARY HOSPITAL OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
302 MILITARY HOSPITAL OF CHINA
Publication Date
2011-01-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a kit for quantitatively detecting hepatitis B virus (HBV) cccDNA (Covalently Closed Circular DNA) of paraffin embedded hepatic tissue, which is characterized by comprising the following steps of: carrying out paraffin embedding on hepatic tissue; then respectively adding extracted total DNA into an HBV rolling loop primer and a notch crossing primer; and carrying out rolling and notch crossing fluorescent quantitative amplification. The kit has the advantages of no need of fresh hepatic tissue as a detecting sample, high detecting specificity and high sensitivity.
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Description

Technical field:

[0001] The invention relates to a method for quantitative detection of hepatitis B virus cccDNA, in particular to a method and a detection kit for quantitative detection of hepatitis B virus cccDNA in trace paraffin-embedded liver tissue. Background technique:

[0002] Hepatitis B virus covalently closed circular DNA (HBV cccDNA) is a key factor in HBV persistent infection and disease recurrence after antiviral drug withdrawal. Quantitative detection of HBV cccDNA is of great significance for evaluating the effect of antiviral treatment in patients with hepatitis B, but it has not been carried out in clinical practice. Two factors limit the practical application of HBV cccDNA testing.

[0003] On the one hand, it is a question of the source of the sample:

[0004] Since the cccDNA of hepatitis B virus mainly exists in the nucleus of liver cells, fresh human liver tissue should be used as a test sample, but fresh liver tissue is not easy to obtain. Unless ...

Claims

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