Aqueous phase preparation method for chitosan-quantum dot fluorescent probe

A technology for the preparation of fluorescent probes and aqueous phases, which is applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, and luminescent materials to achieve good biocompatibility, high fluorescence quantum yield, and good cell compatibility

Inactive Publication Date: 2011-02-02
WUHAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, as a nanomaterial, due to its own physical and chemical properties and the influence of environmental factors, quantum dots also show certain biological toxicity effects in the application process. How to improve the stability and biocompatibility of quantum dot fluorescent probes Sex is an urgent issue

Method used

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  • Aqueous phase preparation method for chitosan-quantum dot fluorescent probe
  • Aqueous phase preparation method for chitosan-quantum dot fluorescent probe
  • Aqueous phase preparation method for chitosan-quantum dot fluorescent probe

Examples

Experimental program
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Embodiment 1

[0021] A kind of aqueous phase preparation method of chitosan-quantum dot fluorescent probe, its concrete steps are as follows:

[0022] 1. Prepare 10g / L carboxymethyl chitosan solution:

[0023] Weigh 1 gram of carboxymethyl chitosan and dissolve it in a beaker with double distilled water. If the dissolution is slow, heat it on a hot plate to close to 100°C. After it is completely dissolved, transfer it to a 100ml volumetric flask. Standby, obtained carboxymethyl chitosan solution.

[0024] 2. Prepare 0.0005mol / L CdTe quantum dot solution:

[0025] Use a pipette to accurately pipette 25mL of CdTe quantum dot (0.002mol / L) solution into a beaker, add twice distilled water to a volume of about 90mL, adjust its pH value to 9 with 0.001mol / L NaOH, and transfer to a 100ml volumetric flask After constant volume, shake well for use, and obtain a CdTe quantum dot solution. (CdTe quantum dots are self-made, the brief process of preparation is as follows: CdCl 2 Solution and thiogly...

Embodiment 2

[0029] A kind of aqueous phase preparation method of chitosan-quantum dot fluorescent probe, its concrete steps are as follows:

[0030] 1. Prepare 5g / L carboxymethyl chitosan solution:

[0031] Weigh 0.5 g of carboxymethyl chitosan and dissolve it in a beaker with twice distilled water. If the dissolution is slow, heat it on a hot plate to close to 100°C. After it is completely dissolved, transfer it to a 100 ml volumetric flask. Standby, obtained carboxymethyl chitosan solution.

[0032] 2. Prepare 0.001mol / L CdTe quantum dot solution:

[0033] Use a pipette to accurately pipette 50mL of CdTe quantum dot (0.002mol / L) solution into a beaker, add twice distilled water to a volume of about 90mL, adjust its pH value to 10 with 0.001mol / L NaOH, and transfer to a 100ml volumetric flask After constant volume, shake well for use, and obtain a CdTe quantum dot solution.

[0034] 3. Synthesis of chitosan-quantum dot fluorescent probes under ultrasonic conditions:

[0035] Mix 5g / L...

Embodiment 3

[0038] A kind of aqueous phase preparation method of chitosan-quantum dot fluorescent probe, its concrete steps are as follows:

[0039] 1. Prepare 2.5g / L carboxymethyl chitosan solution:

[0040] Weigh 0.25g of carboxymethyl chitosan and dissolve it in a beaker with twice distilled water. If the dissolution is slow, heat it on a hot plate to close to 100°C. After it is completely dissolved, transfer it to a 100ml volumetric flask. Standby, obtained carboxymethyl chitosan solution.

[0041] 2. Prepare 0.0005mol / L CdTe quantum dot solution:

[0042] Use a pipette to accurately pipette 25mL of CdTe quantum dot (0.002mol / L) solution into a beaker, add twice distilled water to a volume of about 90mL, adjust its pH value to 11 with 0.001mol / L NaOH, and transfer to a 100ml volumetric flask After constant volume, shake well for use, and obtain a CdTe quantum dot solution.

[0043] 3. Synthesis of chitosan-quantum dot fluorescent probes under oscillating conditions:

[0044] Mix 2...

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Abstract

The invention discloses an aqueous phase preparation method for a chitosan-quantum dot fluorescent probe, which comprises the following steps of: A, preparing solution of carboxymethyl chitosan: weighing carboxymethyl chitosan, dissolving in a beaker by using double distilled water, and fixing the volume in a bottle for later use; B, preparing solution of CdTe quantum dot: according to the concentration of CdTe quantum dot, transferring the CdTe quantum dot solution to the beaker by using a transfer pipette, adding double distilled water, regulating the pH value to be 7-13, and fixing the volume in a bottle for later use; and C, mixing the prepared carboxymethyl chitosan solution and the CdTe quantum dot solution according to different volume ratios, shaking uniformly and standing at room temperature, or performing ultrasonic wave reaction, oscillating reaction or microwave heating reaction to obtain chitosan-quantum dot fluorescent probes with corresponding luminous wavelengths and different fluorescence intensities. The preparation method has the advantages of simple operation, good water solubility of products, long stabilization time, high yield of fluorescence quantum, good cellular compatibility, and wide application in the aspects of cell and macro-molecular biomarkers.

Description

technical field [0001] The invention belongs to the field of nanomaterial modification technology and biological analysis and detection technology, and more specifically relates to a method for preparing a chitosan-quantum dot fluorescent probe in an aqueous phase. The prepared chitosan-quantum dot fluorescent probe is suitable for cells and organisms Fluorescent labeling of macromolecular systems (proteins, nucleic acids, etc.). Background technique [0002] Chitosan is the product of deacetylation of chitin. There are many hydroxyl groups and amino groups distributed on its macromolecular chain, and it contains hydrophilic groups and hydrophobic groups. At the same time, the hydrogen bonds between the backbone chains form the secondary structure of chitosan macromolecules. The particularity of the structure of chitosan and the physical properties of polymer compounds make it have the functions of ion exchange, chelation and adsorption with many ions, organic substances ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/88C08B37/08G01N21/64C08L5/08
Inventor 周培疆何振宇吴新国肖玲吴迪
Owner WUHAN UNIV
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