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Quality analysis method for loins-strengthening and kidney-invigorating oral liquid

A technology for kidney oral liquid and quality analysis, applied in the field of quality control of traditional Chinese medicine preparations, can solve problems such as large interference, many impurities, and interference of medicinal ingredients, and achieve the effects of high sensitivity, ensuring curative effect, and controlling quality.

Active Publication Date: 2012-05-30
GUANGZHOU XIANGXUE PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Zhuangyaojianshen Oral Liquid was extracted by using the extraction method reported in the literature, and the results showed that there were many impurities in the sample, and the interference was large, and formononetin could not be detected, and the chromatographic peaks could not reach the baseline separation
This is due to the fact that different products contain different medicinal materials, and the components of medicinal materials interfere with each other. The method reported in the literature is used for preparation and determination, and the ideal separation effect cannot be obtained.

Method used

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  • Quality analysis method for loins-strengthening and kidney-invigorating oral liquid
  • Quality analysis method for loins-strengthening and kidney-invigorating oral liquid
  • Quality analysis method for loins-strengthening and kidney-invigorating oral liquid

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Experimental program
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Effect test

Embodiment 1

[0019] Embodiment 1 A kind of quality analysis method of Zhuangyaojianshen oral liquid

[0020] Adopt HPLC to measure the content of formononetin, comprising the following steps:

[0021] a. Prepare the test solution

[0022] Take 40ml of Zhuangyao Jianshen Oral Liquid, add petroleum ether to extract 2-3 times, 60ml each time, discard petroleum ether; add chloroform to extract 2-3 times, 60ml each time, combine the extracts, evaporate to dryness, and the residue Add methanol to dilute to 10ml, shake well, filter, and use as the test solution;

[0023] b. Preparation of reference solution

[0024] Take the formononetin reference substance, add methanol to make 1ml of a solution containing 20 μg of formononetin, and obtain the reference substance solution;

[0025] c. Determination

[0026] Chromatographic conditions: use octadecylsilane bonded silica gel as filler, acetonitrile-1% formic acid solution with a volume ratio of 38-42:58-62 as mobile phase; detection wavelength ...

Embodiment 2

[0031] Example 2 Condition screening and methodological verification of a quality analysis method for Zhuangyaojianshen Oral Liquid

[0032] 1. Screening of HPLC chromatographic conditions

[0033] The existing literature reports that there is absorption at 249nm and 254nm, and the spectrum measured by the diode array detector shows that there is absorption at 249nm, 254nm, 290nm, and 327nm, and the maximum absorption is 290nm. At this wavelength, the sensitivity is high and the peak shape is sharp. , the separation degree is greater than 1.5, the peak time is within 10 minutes, and the detection time is saved, so the chromatographic conditions are determined as follows: use octadecylsilane bonded silica gel as a filler, and use 38~42:58~62 acetonitrile-1% Formic acid solution is the mobile phase; the detection wavelength is 290nm. The number of theoretical plates calculated based on the peak of formononetin should not be less than 4000.

[0034] 2. Preparation of the test a...

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Abstract

The invention discloses a quality analysis method for loins-strengthening and kidney-invigorating oral liquid. The content of formononetin is measured by high performance liquid chromatography (HPLC), and the method comprises the steps of: preparing test solution, preparing reference solution and measuring. The method controls quality of the loins-strengthening and kidney-invigorating oral liquidby measuring the content of formononetin in the loins-strengthening and kidney-invigorating oral liquid through the HPLC, has high sensitivity, high specificity, no interference on negative control, has the accuracy, repeatability, linear relation and stability which can meet the requirements of scientific research and production, can more effectively control the product quality, and ensures the curative effect of medicaments.

Description

technical field [0001] The invention belongs to the field of quality control of traditional Chinese medicine preparations, in particular to a quality analysis method for Zhuangyao Jianshen oral liquid. Background technique [0002] The oral liquid for strengthening the waist and invigorating the kidney currently on the market is generally made of medicinal flavors such as Spatholobus Spatholobus, Gouji, Sangjishen, Fructus Rosa, and Ligustrum lucidum. , for kidney deficiency lumbago, rheumatic bone pain, weak knees, frequent urination. Clinical verification shows that the effective rate of this product in treating patients with kidney deficiency and low back pain is 95.0%, and the effective rate is 58.3%; the effective rate in patients with lumbar hyperosteogeny is 94.8%. All have obvious improvement effect. The medicinal material of Caulis Spatholobus in the prescription has the effects of nourishing blood, invigorating blood circulation, and dredging collaterals, and is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02A61K36/738A61K9/00A61P13/12A61P29/00A61P19/08
Inventor 连林生李小妹康志英朱小燕蔡春玲
Owner GUANGZHOU XIANGXUE PHARMA CO LTD