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Application of Ulinastatin in preparing drug for curing autoimmune encephalomyelitis and pharmaceutical composition thereof

An autoimmune, ulinastatin technology, applied in the field of medicinal chemistry, can solve problems such as increased neuronal loss, side effects infection, etc.

Active Publication Date: 2013-09-25
GUANGDONG TECHPOOL BIO-PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, MS drug therapy is mainly aimed at anti-inflammation and immune regulation. The standard classic treatment in the acute phase is high-dose hormone shock therapy, which is often accompanied by serious side effects such as infection, gonadal suppression, and tumors, and inhibits endogenous brain protection pathways. , increase neuron loss, so it is necessary to find safer and more effective therapeutic drugs

Method used

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  • Application of Ulinastatin in preparing drug for curing autoimmune encephalomyelitis and pharmaceutical composition thereof
  • Application of Ulinastatin in preparing drug for curing autoimmune encephalomyelitis and pharmaceutical composition thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1 Preparation of ulinastatin dry powder injection

[0013] Take 100 million units of filter-sterilized Ulinastatin aqueous solution, add 20 grams of mannitol to dissolve it, adjust the pH to neutral, add water for injection to 2000 ml, add sodium chloride to adjust isotonicity, filter sterilely, and divide into 1000 In a vial, freeze-dried under aseptic conditions to obtain.

Embodiment 2

[0014] Example 2 Preparation of Ulinastatin Injection

[0015] Take 100 million units of filter-sterilized Ulinastatin aqueous solution, add 20 g of mannitol to dissolve it, adjust the pH to neutral, add water for injection to 2000 ml, add sodium chloride to adjust isotonicity, filter sterilely, and dispense 1,000 Get it in a vial.

Embodiment 3

[0016] Example 3 Ulinastatin's effect on experimental autoimmune encephalomyelitis

[0017] (experimental autoimmune encephalomyelitis, EAE) therapeutic effect in mice

[0018] Part 1: Establishment of EAE mouse model

[0019] Take 6-8 weeks old C57BL / 6 female mice, weighing (18-20) g, first prepare the following preparations:

[0020] Solution A: MOG35-55, 0.2 mg per mouse, dissolved in sterile endothelin-free PBS.

[0021] Liquid B: tuberculin H37RA, 0.4 mg per mouse, dissolved in complete Freund's adjuvant containing tuberculin H37RA.

[0022] Liquid A is emulsified in liquid B with an emulsifier for more than 30 minutes and fully emulsified to make a MOG emulsion.

[0023] After disinfection of the skin of the mice, the emulsion was injected subcutaneously at multiple points in the lower abdomen on both sides. On day 0 and 48 hours, the pertussis toxin was injected again, 0.4 μg per mouse (dissolved in 0.1 ml of PBS), and on the 7th day, the same amount of MOG emulsion was injected s...

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Abstract

The invention relates to an application of Ulinastatin in preparing a drug for curing autoimmune encephalomyelitis, and the drug related by the invention is derived from natural protein drugs purified by human urine, has stable and reliable quality and less adverse effects, and overcomes the defects of more side effects, influenced life quality of patients even caused death and the like existing in the common drugs such as glucocorticoid and the like for curing autoimmune encephalomyelitis. Contrastive studies of curative effects on animal models of the autoimmune encephalomyelitis find that Ulinastatin has the same curative effect as hormone.

Description

Technical field [0001] The invention relates to the field of medicinal chemistry. Specifically, the present invention relates to a medicine for treating autoimmune encephalomyelitis, and particularly relates to an application of ulinastatin as a medicine for preparing and treating autoimmune encephalomyelitis. Background technique [0002] Multiple Sclerosis (MS) is one of the most common neuroimmune diseases, with a prevalence of close to three thousandths in the temperate zone. Young people are susceptible to the disease, and the age of onset is about 28 years old. It has the characteristics of prolongation, recurrence and gradual deterioration. 50% of patients need to use crutches to walk 15 years after the first onset, and the mortality rate is high. Therefore, the disease is extremely harmful. As the pathogenesis of MS is unclear so far, there is no effective treatment. [0003] MS pathologically has inflammation infiltration, demyelination and neurodegeneration. Its pathog...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/57A61K9/19A61K9/08A61P37/02A61P25/00
Inventor 傅和亮吴蓉蓉邹春芽陈颖华明娟
Owner GUANGDONG TECHPOOL BIO-PHARMA CO LTD
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