Ultrasound enzyme-assisted solanesol extracting method
A technology of auxiliary extraction and solanesol, applied in the separation/purification of hydroxyl compounds, organic chemistry, fermentation, etc., can solve the problems of environmental pollution of lye, easy degradation or isomerization of solanesol, and achieve waste liquid discharge. small amount of effect
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Embodiment 1
[0022] The method for assisting extraction of solanesol by ultrasonic enzymatic method of the present invention includes the following steps:
[0023] 1) After crushing the dried potato leaves through a 40-mesh sieve, weigh 40g of the dried potato leaves, add 600ml of distilled water, and wash with ultrasonic water for 30min under the conditions of ultrasonic power 150W, frequency 40KHz, temperature 30℃, and filter to obtain solid substance A;
[0024] 2) Add 400ml of cellulase solution prepared with pH4.0 disodium hydrogen phosphate-citric acid buffer solution to substance A at a concentration of 0.001g / ml cellulase solution at an ultrasonic power of 100W, a frequency of 40KHz, a temperature of 50℃, and a stirring speed of 120r Under the condition of ultrasonic assisted enzymatic hydrolysis for 15 minutes, and centrifuge for 10 minutes under the condition of 4000r / min, discard the supernatant to obtain substance B;
[0025] 3) Add 200ml of lipase solution with a concentration of 0.0...
Embodiment 2
[0028] The method for assisting extraction of solanesol by ultrasonic enzymatic method of the present invention includes the following steps:
[0029] 1) After crushing the dried potato leaves through a 60-mesh sieve, weigh 50g of the potato leaves, add 250ml of distilled water, and wash with ultrasonic water for 40 minutes at an ultrasonic power of 200W, a frequency of 40KHz, and a temperature of 40°C to obtain solid substance A after filtration;
[0030] 2) Add 250ml of cellulase solution prepared with pH 4.4 disodium hydrogen phosphate-citrate buffer solution to substance A at a concentration of 0.004g / ml cellulase solution, at an ultrasonic power of 150W, frequency of 40KHz, temperature of 40℃, and stirring speed of 110r Under the condition of ultrasonic assisted enzymatic hydrolysis for 30 min at 3800r / min, centrifuge at 3800r / min for 12min, and discard the supernatant to obtain substance B;
[0031] 3) Add 500ml of lipase solution with a concentration of 0.004g / ml prepared with...
Embodiment 3
[0034] The method for assisting extraction of solanesol by ultrasonic enzymatic method of the present invention includes the following steps:
[0035] 1) After crushing the dried potato leaves through a 50-mesh sieve, weigh 20g of the potato leaves, add 400ml of distilled water, and wash with ultrasonic water for 50min under the conditions of ultrasonic power 250W, frequency 40KHz, temperature 50℃, and filter to obtain solid substance A;
[0036] 2) Add 300ml of cellulase solution prepared with pH 4.8 disodium hydrogen phosphate-citric acid buffer to substance A at a concentration of 0.002g / ml cellulase solution, at an ultrasonic power of 200W, frequency of 40KHz, temperature of 30℃, and stirring speed of 100r Ultrasonic-assisted enzymatic hydrolysis for 45 min under the condition of / min, and centrifugation under the condition of 3600r / min for 15 min, the supernatant was discarded to obtain substance B;
[0037] 3) Add 500ml of lipase solution prepared with pH 8.3 disodium hydrogen ...
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