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In vitro quick breeding method of seedling stem of santal seed embryo

A technology of sandalwood and stem segments, which is applied in the field of in vitro rapid propagation of the stem segments of sandalwood seedlings, can solve the problems of low rooting rate of sandalwood seedlings and large-scale production of sandalwood seedlings, and improve the survival rate and reproduction coefficient of transplanting High, the effect of suppressing the phenomenon of defoliation

Active Publication Date: 2011-03-09
DONGGUAN CITY RUISHEN BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention effectively solves the problem of low rooting rate of sandalwood seedlings and the large-scale production of sandalwood seedlings, and solves the bottleneck technology of sandalwood tissue culture production

Method used

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  • In vitro quick breeding method of seedling stem of santal seed embryo
  • In vitro quick breeding method of seedling stem of santal seed embryo
  • In vitro quick breeding method of seedling stem of santal seed embryo

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Experimental program
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Embodiment 1

[0034] The in vitro rapid propagation method of sandalwood seedling stem section is as follows: figure 1 , including the following steps:

[0035] (1) Seed embryo seedling: get a small amount of mature sandalwood fruit (purchased from Guangdong South Medicine Base), wash with clear water, and wash with 0.1% HgCl 2 Soak and sterilize for 10 minutes, take out the sandalwood fruit, rinse with sterile water and blot dry with sterile filter paper. Cut the sandalwood fruit open with a scalpel and remove the seeds. Place in 50 milliliters of 1% pectinase (sigma company, produced in the United States) + 50 milliliters of 3% cellulase R-10 (Onoznka, produced in Japan) enzyme solution (pH5.0) and hydrolyze at 30 ° C for 24 hours, and take out the seeds , rinsed with sterile water and blotted dry, then disinfected with 8% sodium hypochlorite for 10 minutes, rinsed with sterile water and blotted dry. Cut the seed shell with a sterile scalpel blade, take out the seed embryo, and place i...

Embodiment 2

[0040] The in vitro rapid propagation method of sandalwood seedling stem section is as follows: figure 1 , including the following steps:

[0041] (1) Seed embryo becomes seedling: with embodiment 1.

[0042] (2) Proliferation of stem seedlings: Cut the grown seedlings into a section according to 2 nodes, and insert the medium into MS+6-BA1.5mg.L -1 +NAA 1mg.L -1 on the culture medium. Place them in a culture room with a light of 3000 lux, a light time of 14 hours, and a temperature of 24°C. After 8 days, 100% of the stem sections germinated axillary buds, and wherein 35% of the stem sections germinated axillary buds at the upper and lower nodes. After 45 days, the axillary buds grew into strong seedlings with an average height of 6.2 cm, with an average of 7.8 nodes (blades); in 2 months, the average seedling height was 8.1 cm, with an average of 11.2 nodes (blades). Proliferate with the new stem segment that grows again, and the multiplication coefficient of 2 months is...

Embodiment 3

[0046] The in vitro rapid propagation method of sandalwood seedling stem section is as follows: figure 1 , including the following steps:

[0047] (1) Seed embryo becomes seedling: with embodiment 1.

[0048] (2) Proliferation of stem segment seedlings: Cut the grown seedlings into a section according to 2 nodes, and insert the medium as MS+6-BA 1.0mg.L -1 +NAA 0.5mg.L -1 on the culture medium. Placed in a culture room with a light of 3000 lux, a light time of 14 hours, and a temperature of 26°C. After 10 days, 100% of the stems will germinate axillary buds, and 65% of the stems will germinate axillary buds at the upper and lower nodes. After 45 days, the axillary buds will grow into healthy and healthy plants with an average height of 5.0cm. Young seedlings have an average of 6.5 nodes (leaves); 2-month-old seedlings have an average height of 7.3 cm and an average of 9.5 nodes (blades). Due to the increase in the number of germinated axillary buds, and then proliferate wi...

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Abstract

The invention discloses a in vitro quick-breeding method of seedling stems of santal seed embryos, which relates to the method of plant tissue cultivation. The method comprises the following steps. (1) Santal seeds are subjected to enzymatic hydrolysis and sterilization in the solution of pectinase and cellulose. Santal seed embryos are separated and cultured under sterile conditions after being taken out of the solution to obtain sterile seedlings of santal seed embryos. Seedling stems of santal seed embryos are cut out and axillary buds are induced to seedling formation. The above seedlings are proliferated for 10-12 generations to increase the number of seedling stems of santal seed embryos. The seedling stems are induced to root induction to obtain rooted seedlings by adding the solutions of AgNO3 and IBA. The rooted seedlings are subjected to greenhouse hardening-seedling and outdoor transplanting successively to obtain survived santal seedlings. The above method breaks the bottleneck of santal tissue cultivation and effectively increases the rooting rate of cultured tissues. By adopting the improvement of tissue culture technology and the optimization of media formulations, the method of the invention is applicable to the large scale production of santal seedlings.

Description

technical field [0001] The invention belongs to the technical field of plant group culture, in particular to a method for in vitro rapid propagation of the stem section of sandalwood seedlings. Background technique [0002] Sandalwood (Santalum album L.), also known as white sandalwood, belongs to the genus Sandalwood and the family Sandalaceae. It grows in tropical areas and is mainly produced in tropical countries such as India, Indonesia, Vietnam, and Australia. Sandalwood is a precious medicinal material, and its medicinal part is the heartwood of the trunk. It has the effects of warming the heart, appetizing and relieving pain. Indications for cold stagnation, coronary heart disease, gelatinous pain, abdominal pain, stomach pain, less food, sexually transmitted diseases, etc., can be anti-inflammatory, anti-bacterial, anti-spasmodic, aphrodisiac, astringent, antitussive, clearing heat and moistening lung, dispelling flatulence, diuresis, treating skin diseases, Hemost...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 廖俊杰李进进
Owner DONGGUAN CITY RUISHEN BIOTECH