Application of T-2 toxin to preparing drugs for treating pancreatic carcinoma
A technology for T-2 and pancreatic cancer, applied in drug combinations, pharmaceutical formulations, anti-tumor drugs, etc., can solve the problem that the research on T-2 toxin has not yet been found.
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Embodiment 1
[0014] Example 1: Experimental research on the therapeutic effect of T-2 toxin on pancreatic cancer
[0015] Human pancreatic cancer tissue fragments with a diameter of 2-3 mm were subcutaneously transplanted into mice. After the tumor grew to 6-7 mm, T-2 toxin dissolved in ethanol saline mixture was injected into the tumor at a dose of 2.5 mg / kg body weight. injection, and the control group was injected with normal saline. The size of the tumor was measured with a vernier caliper or a standard template on the 1st, 5th, 9th, 14th, 19th, and 24th day after injection, and the measurement lasted for 24 days. After 24 days of treatment, the tumor volume of the animals in the T-2 toxin group was only 52.19% of that in the control group.
Embodiment 2
[0016] Example 2: Experimental research on the therapeutic effect of T-2 toxin on lung cancer
[0017] Mouse Lewis lung cancer cells were placed in complete culture medium containing 10% fetal bovine serum and DMEM, at 37°C, 5% CO 2 Cultivate in an incubator, and replace the culture medium every 2-3 days. The cells grow adherently in a single layer, digested with 0.5% trypsin and passaged. After the total number of cells reaches the requirement, the number of living cells determined by the trypan blue exclusion method is greater than 95%, and the cell concentration is adjusted to 5×10 6 / ml, cell suspension spare C57BL / 6 pure line mice, male, 20, 6-8 weeks old, body weight (18±1) g. Inoculate subcutaneously in the axilla. Each mouse was inoculated with 0.2ml, and the cell count was 5×10 6 cells / ml. Mice were randomly divided into 2 groups, 10 in each group. In the control group, 2 ml of normal saline was injected into the tumor, and in the experimental group, T-2 toxin d...
Embodiment 3
[0021] Example 3: Experimental research on the therapeutic effect of T-2 toxin on liver cancer
[0022] Mouse H 22 Liver cancer cell lines, ICR mice peritoneal passage. Under aseptic conditions, the H 22 For liver cancer cells, adjust the cell concentration to 5×10 with normal saline 6 / ml, 20 detergent ICR mice, 6-8 weeks old, male, 18-22g, were randomly divided into 2 groups, 10 in each group. 0.2 ml of tumor cell suspension was inoculated subcutaneously in the left armpit. Seven days after the inoculation, the control group was injected with 2 ml of normal saline, and the experimental group was injected with T-2 toxin dissolved in ethanol-normal saline mixture at a dose of 2.0 mg / kg body weight. The long diameter (L) and short diameter (W) of the tumor were measured with a vernier caliper every other day after group administration, and the tumor volume and tumor growth inhibition rate were calculated. The results are shown in Table 2. The test results showed that T-2 t...
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