Cyclodextrin-polyethyleneimine-mediated supramolecular delivery system and preparation method
A polyethyleneimine and cyclodextrin technology, which is applied in the field of non-viral gene transfection vectors, can solve the problems of high cytotoxicity, limited application scope of viral vectors, low targeting, etc., and achieves low toxicity and strong transformation. , the effect of increasing the function
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Embodiment 1
[0060] Example 1: Take β-cyclodextrin as an example.
[0061] 1) Preparation of polyethyleneimine-cyclodextrin polymer
[0062] ●Weigh 5 g of β-cyclodextrin and dissolve it in 50 ml of dimethyl sulfoxide, weigh 10 g of N,N'-carbonyldiimidazole and dissolve it in 20 ml of dimethyl sulfoxide, and store in the dark under the protection of nitrogen Mix N,N'-carbonyldiimidazole solution with cyclodextrin solution, add 300 microliters of triethylamine, stir for 2 hours, weigh 15 grams of polyethyleneimine (PEI600) with a molecular weight of 600, and dissolve it in 30 In a milliliter of dimethyl sulfoxide, the solution was slowly added dropwise to the activated cyclodextrin solution in a dark place under the protection of nitrogen, and the dropwise addition was completed within 2 hours. After the dropwise addition was completed, the reaction was continued for another 3 hours. After the reaction, the solution was dialyzed in a 8000-14000 dialysis bag for 48 hours, and then freeze-dri...
Embodiment 2
[0079] Embodiment 2: Taking α-cyclodextrin as an example
[0080] 1) Preparation of polyethyleneimine-cyclodextrin polymer:
[0081] ●Weigh 10 g of α-cyclodextrin and dissolve in 60 ml of dimethyl sulfoxide, weigh 20 g of N,N'-carbonyldiimidazole and dissolve in 30 ml of dimethyl sulfoxide, and store in the dark under the protection of nitrogen Mix N,N'-carbonyldiimidazole solution with cyclodextrin solution, add 300 microliters of triethylamine, stir and react for 2 hours, weigh 10 grams of polyethyleneimine (PEI1200) with a molecular weight of 1200, and dissolve it in 20 In a milliliter of dimethyl sulfoxide, the solution was slowly added dropwise to the activated cyclodextrin solution in a dark place under the protection of nitrogen, and the dropwise addition was completed within 2 hours. After the dropwise addition was completed, the reaction was continued for another 3 hours. After the reaction, the solution was dialyzed in a 8000-14000 dialysis bag for 48 hours, and the...
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