Cyclodextrin-polyethyleneimine-mediated supramolecular delivery system and preparation method

A polyethyleneimine and cyclodextrin technology, which is applied in the field of non-viral gene transfection vectors, can solve the problems of high cytotoxicity, limited application scope of viral vectors, low targeting, etc., and achieves low toxicity and strong transformation. , the effect of increasing the function

Active Publication Date: 2012-07-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently widely used viral vector-mediated system, although it has a high gene transfection efficiency, but high cytotoxicity, low targeting, DNA carrying capacity limitations, production packaging and high prices and other issues limit The scope of application of viral vectors, especially in 1999, the death of "Jesse Gelsinger" caused by the use of adenoviral vectors in the clinical trials of gene therapy in the United States made researchers more cautious in the clinical use of viral vectors

Method used

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  • Cyclodextrin-polyethyleneimine-mediated supramolecular delivery system and preparation method
  • Cyclodextrin-polyethyleneimine-mediated supramolecular delivery system and preparation method
  • Cyclodextrin-polyethyleneimine-mediated supramolecular delivery system and preparation method

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Experimental program
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Effect test

Embodiment 1

[0060] Example 1: Take β-cyclodextrin as an example.

[0061] 1) Preparation of polyethyleneimine-cyclodextrin polymer

[0062] ●Weigh 5 g of β-cyclodextrin and dissolve it in 50 ml of dimethyl sulfoxide, weigh 10 g of N,N'-carbonyldiimidazole and dissolve it in 20 ml of dimethyl sulfoxide, and store in the dark under the protection of nitrogen Mix N,N'-carbonyldiimidazole solution with cyclodextrin solution, add 300 microliters of triethylamine, stir for 2 hours, weigh 15 grams of polyethyleneimine (PEI600) with a molecular weight of 600, and dissolve it in 30 In a milliliter of dimethyl sulfoxide, the solution was slowly added dropwise to the activated cyclodextrin solution in a dark place under the protection of nitrogen, and the dropwise addition was completed within 2 hours. After the dropwise addition was completed, the reaction was continued for another 3 hours. After the reaction, the solution was dialyzed in a 8000-14000 dialysis bag for 48 hours, and then freeze-dri...

Embodiment 2

[0079] Embodiment 2: Taking α-cyclodextrin as an example

[0080] 1) Preparation of polyethyleneimine-cyclodextrin polymer:

[0081] ●Weigh 10 g of α-cyclodextrin and dissolve in 60 ml of dimethyl sulfoxide, weigh 20 g of N,N'-carbonyldiimidazole and dissolve in 30 ml of dimethyl sulfoxide, and store in the dark under the protection of nitrogen Mix N,N'-carbonyldiimidazole solution with cyclodextrin solution, add 300 microliters of triethylamine, stir and react for 2 hours, weigh 10 grams of polyethyleneimine (PEI1200) with a molecular weight of 1200, and dissolve it in 20 In a milliliter of dimethyl sulfoxide, the solution was slowly added dropwise to the activated cyclodextrin solution in a dark place under the protection of nitrogen, and the dropwise addition was completed within 2 hours. After the dropwise addition was completed, the reaction was continued for another 3 hours. After the reaction, the solution was dialyzed in a 8000-14000 dialysis bag for 48 hours, and the...

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Abstract

The invention relates to a carrier of non-virogene transfection, in particular to a cyclodextrin-polyethyleneimine material-mediated supramolecular delivery system, and aiming to provide a novel supramolecular delivery system for non-virogene treatment and medicine synergistic treatment. The supramolecular delivery system is formed through combining polyethyleneimine-cyclodextrin with adamantane-adriamycin by utilizing a self-assembly principle with the polyethyleneimine-cyclodextrin as a framework and the adamantane-adriamycin as a core. The supramolecular delivery system has the advantages that a self-assembled composite can reduce toxic and side effects of adriamycin, simultaneously improve the bioavailability of adriamycin and can carry out synergistic effect together with a gene carried by the polyethyleneimine-cyclodextrin.

Description

technical field [0001] The invention relates to a class of non-viral gene transfection carrier, in particular to a supramolecular delivery system mediated by a cyclodextrin-polyethyleneimine material. Background technique [0002] Tumor is a common and frequently-occurring disease that seriously threatens human health, and overcoming it has always been a research hotspot attracting worldwide attention. In 1967, Tan et al. [1] reported for the first time that doxorubicin has strong anti-tumor activity and can be used to treat leukemia and lymphoma. Subsequent studies have shown that doxorubicin can well inhibit tumors as a chemotherapy drug and is an excellent chemotherapy drugs [2-5]. However, it was immediately discovered that doxorubicin has severe side effects on the human body, and it can also bring side effects such as cardiotoxicity and hair loss to patients during treatment, which seriously affects the quality of life of patients [1]. However, in addition to killing...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/48A61K47/40A61K31/704A61K48/00A61P35/00A61K47/61
Inventor 汤谷平胡奇达胡秀荣范辉周峻
Owner ZHEJIANG UNIV
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