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Process for extracting ginkgo leaf polysaccharide with aid of ultrasonic

An auxiliary extraction and extraction process technology, applied in the field of extraction of ginkgo biloba polysaccharides, can solve the problem of low extraction rate, achieve high extraction rate, improve transfer rate, and increase the effect of contact area

Inactive Publication Date: 2011-04-06
蒋熠霞
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Problems solved by technology

However, the active substances in Ginkgo biloba are concentrated in fumigone, and the extraction methods mainly include water extraction and alcohol precipitation method, and this enzymatic method combined with water extraction and alcohol method. These methods are not only low in extraction rate, but also time-consuming.

Method used

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Embodiment Construction

[0006] The present invention will be further described below in conjunction with embodiment:

[0007] Raw materials: use the natural fallen leaves of ginkgo after autumn

[0008] Instruments: RE-85Z rotary evaporator, JH223 visible light spectrophotometer, SKB-Ⅱ circulating water multipurpose vacuum pump, DHG-9143Ⅱ type electric heating constant temperature blast drying oven, JFSD-100-Ⅱ pulverizer, JY92-ⅡD ultrasonic cell pulverizer .

[0009] Process flow: Ginkgo biloba leaves after autumn→drying→crushing and sieving (40 mesh)→ultrasonic treatment (distilled water as solvent, temperature controlled at 80°C)→centrifugation (removing slag)→vacuum concentration (to 1 / 10 before concentration)→ Alcohol precipitation → centrifugation (take precipitation) → vacuum freeze-drying → crude polysaccharide.

[0010] GBLP content determination method: take 100mg GBLP crude product, dilute to 100ml with distilled water, and accurately draw 1.0ml equivalent volume from it, which is the cru...

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Abstract

The invention provides a process for extracting ginkgo leaf polysaccharide with the aid of ultrasonic. The extracting process is technically characterized by comprising the following steps of: drying ginkgo leaves (ginkgo leaves after autumn), crushing and sieving (40 meshes), performing ultrasonic treatment (the distilled water is used as a solvent, and the temperature is controlled at 80 DEG C), centrifuging (removing residue), performing vacuum concentration (till 1 / 10 before concentration), performing alcohol precipitation, centrifuging (taking sediment), and performing vacuum freezing and drying to obtain a crude polysaccharide product. The process has the advantages that: the ultrasonic is acted on the liquid to generate cavatition during production to cause bubble formation, increment and blasting compression so as to disperse a solid sample, increase the contact area between the sample and the extracting solvent, improve the transfer rate of a target substance from solid phase to liquid phase, shorten the extracting time, reduce energy consumption and improve the extracting rate.

Description

technical field [0001] The invention belongs to a novel technique for extracting ginkgo leaf polysaccharide, in particular to a technique for extracting ginkgo leaf polysaccharide by using ultrasonic technology. Background technique [0002] Ginkgo biloba polysaccharide has anti-aging, immune regulation and anti-tumor effects, and has potential medicinal value and pharmaceutical research and development. However, the active substances in Ginkgo biloba are concentrated in fumigone substances, and the extraction methods mainly include water extraction and alcohol precipitation method, and this enzymatic method combined with water extraction and alcohol method. These methods are not only low in extraction rate, but also time-consuming. Waste of energy. Contents of the invention [0003] The purpose of the present invention is to provide a kind of ultrasonic extraction technology of Ginkgo biloba polysaccharide for the problems existing in the above-mentioned technology [0...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 蒋熠霞
Owner 蒋熠霞
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