Kit for detecting concentration of seminal plasma fructose and detection method
A sugar concentration and kit technology, which is applied in material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problems of difficulty in widespread use, many operation steps, and expensive reagents. , to achieve stable and reliable results, easy operation, and saving reagent consumption.
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Embodiment 1
[0019] Embodiment 1: a kind of detection kit of seminal berry fructose concentration of the present invention comprises the mixed solution of fructose standard solution, resorcinol (concentration is 2g / L) and hydrochloric acid (concentration is 10mol / L); wherein said resorcinol The mixed solution of diphenol and hydrochloric acid has a volume ratio of resorcinol and hydrochloric acid of 1:3; the concentration of the fructose standard solution is 2 g / L. Calculated on the basis that each kit is loaded with 100 detection reagents required for testing, the fructose standard solution is 0.5ml±20μl, and the mixed solution of resorcinol and hydrochloric acid is 100ml±4ml.
Embodiment 2
[0020] Embodiment 2: a kind of method for detecting the concentration of seminal berry fructose with a kind of detection kit of seminal berry fructose concentration in embodiment 1, the concrete steps of this method are as follows:
[0021] 1) Setting detection instrument parameters: the detection instrument is a semi-automatic biochemical analyzer, and its parameters are: the item type is the end point method; the dominant wavelength is 492nm; the delay time is 3s; the measurement time is 3s; the item unit is g / L ; Standard concentration is 2g / L; Cuvette temperature is 37°C;
[0022] 2) Add the mixed solution of resorcinol and hydrochloric acid in the blank tube, add the mixed solution of standard substance and resorcinol and hydrochloric acid in the standard tube, add the mixed solution of seminal plasma and resorcinol and hydrochloric acid in the sample tube Mixed liquid; wherein the seminal plasma is the supernatant obtained by centrifuging the liquefied semen at 3000 g fo...
Embodiment 3
[0025] Embodiment 3: according to the detection method of embodiment 2, the reaction temperature suitable for the reaction system is determined
[0026] Take 20 μl of fructose standard solution plus 1ml of a mixture of resorcinol and hydrochloric acid, place them in a water bath at 70, 80, and 90°C for 10 minutes, cool in running water, measure color at 492nm, and read the results. It was found that there was no significant difference in the reaction results at different temperatures, and the lower the temperature, the lower the risk, so a 70°C water bath was chosen.
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