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Preparation method and low-temperature continuous stirring ultrafiltration device for anti-tumor and anti-infection allicin injection

A technology of allicin and injection, which is applied in the direction of antineoplastic drugs, devices for making drugs into special physical or taking forms, biochemical equipment and methods, and can solve the problem of low alliin or allicin content, etc. problem, to achieve the effect of reducing decomposition

Active Publication Date: 2011-05-18
新疆胡蒜研究院(有限公司)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of alliin or allicin in garlic preparations listed at home and abroad is very low

Method used

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  • Preparation method and low-temperature continuous stirring ultrafiltration device for anti-tumor and anti-infection allicin injection
  • Preparation method and low-temperature continuous stirring ultrafiltration device for anti-tumor and anti-infection allicin injection
  • Preparation method and low-temperature continuous stirring ultrafiltration device for anti-tumor and anti-infection allicin injection

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Figure 1-3 For the embodiment schematic diagram of the present invention, the preparation method of allicin injection is as follows:

[0050] (1) Add about 1000-2000ml of deoxygenated pure water to a 5000ml rigid three-necked bottle A, add 35.4g of allinase, and stir (M, 60rpm) to dissolve. Adjust the temperature of water bath I to 25-35°C, and check that the pH is 6.5-8.5.

[0051] (2) Put about 500-1000ml of deoxygenated pure water in a closed graduated separatory funnel, add a specified amount of alliin 35.4g, and shake gently to dissolve. High-purity nitrogen or argon fills the G space of the three-neck flask.

[0052] (3) Under continuous stirring (M, 60rpm), the alliin solution enters the three-necked bottle A through the closed graduated separatory funnel through the B port at a speed of about 50ml / min. Continue to stir for 10 minutes after the dropwise addition, and the total reaction time is about 30 minutes. An enzymolysis reaction solution containing all...

Embodiment 2

[0057] The preparation method of allicin injection is as follows:

[0058] (1), add deoxygenated pure water and allinase in the container, wherein deoxygenated pure water: allinase=1000ml: 35.4g, fully stir to fully dissolve allinase, the stirring speed is 60rpm, adjust the water bath temperature to 25°C, Control the pH value within the range of 6.5, and the allinase activity is greater than 1000IU / g; the deoxygenated pure water is freshly boiled and cooled pure water, and high-purity nitrogen or argon gas is injected into the bottom for 1 hour / 10L to remove the dissolved oxygen.

[0059] (2), prepare alliin solution, wherein alliin: deoxygenated pure water = 35.4: 500ml, slowly drop the alliin solution into the above container, wherein alliin: allinase = 1mg: 1IU, fully stir, The stirring speed is 60rpm, and argon or high-purity nitrogen is continuously introduced to fill the container with high-purity nitrogen or argon; the purity of alliin is greater than 90%;

[0060] (3...

Embodiment 3

[0066] (1), add deoxygenated pure water and allinase in the container, wherein deoxygenated pure water: allinase=2000ml: 35.4g, fully stir to make allinase fully dissolve, the stirring speed is 120rpm, adjust the water bath temperature to 35°C, Control the pH value within the range of 8.5, and the allinase activity is greater than 1000IU / g; the deoxygenated pure water is freshly boiled and cooled pure water, and high-purity nitrogen or argon gas is passed into the bottom for 1 hour / 10L to remove the dissolved oxygen.

[0067] (2), prepare alliin solution, wherein alliin: deoxygenated pure water = 35.4: 1000ml, slowly drop the alliin solution into the above container, wherein alliin: allinase = 1mg: 2IU, fully stir, The stirring speed is 60rpm, and argon or high-purity nitrogen is continuously introduced to fill the container with high-purity nitrogen or argon; the purity of alliin is greater than 90%;

[0068] (3), continue to stir after dropwise addition, total reaction time...

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Abstract

The invention discloses a preparation method and low-temperature continuous stirring ultrafiltration device for an anti-tumor and anti-infection allicin injection. The preparation method for the anti-tumor and anti-infection allicin comprises the following two steps: 1. extracting allicin; 2. in a clean environment, diluting the allicin extracted in the step 1 by utilizing a dissolvent precooled to 0-4 DEG C to obtain an allicin solution; and packaging the allicin into allicin injections of different specifications according to ampoule required to fill nitrogen or argon. The anti-tumor and anti-infection allicin injection can be used for intravenous drip to treat diseases such as sepsis or tumor and the like. The invention simultaneously discloses a low-temperature continuous stirring ultrafiltration device for preparing the allicin injection. The invention has the advantages that the formula is reasonable, and the preparation technology is simple; the defects of the prior art are overcome; and the product purity is high, and the performance is stable.

Description

technical field [0001] The invention relates to the field of pharmacy, in particular to a preparation method of anti-tumor and anti-infection allicin injection and a low-temperature continuous stirring ultrafiltration device thereof. Background technique [0002] Garlic (Alliium sativun L.) and its preparations have been included in the US and EU pharmacopoeias. Confirm that alliin (Alliin) and allicin (Allicin) are the main functional ingredients. But the content of alliin or allicin in the garlic preparations listed at home and abroad is very low. The development of safe, effective and quality-controllable high-efficiency new garlic preparations has become a research hotspot for pharmacists from all over the world. [0003] 1. Alliin chemical name: S-Allyl-L-cysteine ​​sulfoxide (S-Allyl-L-cysteine). Melting point: 163-165°C (decomposition, carbonization), [α]D=+60o, white powder, almost odorless. [0004] C 6 h 11 NO 3 S=177.22 [0005] According to the inventio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/08A61K31/255C12P11/00A61P35/00A61P31/04A61P31/10
CPCA61K9/0019A61K9/08C12Y404/01004A61J3/00C12P11/00A61K31/255A61P31/04A61P31/10A61P35/00
Inventor 陈坚李新霞
Owner 新疆胡蒜研究院(有限公司)
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