Method for rapidly breeding sugarcane stem tip by tissue culture and virus removal

A technology for shoot tips and sugar cane, which is applied in the field of plant detoxification culture and rapid propagation, can solve the problems of waste of manpower and material resources, low survival rate of shoot tip meristems, easy variation, etc., so as to improve yield and quality, and solve germplasm degradation. , morphologically normal effect

Inactive Publication Date: 2011-06-15
广州甘蔗糖业研究所湛江甘蔗研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the problem that above-mentioned approach exists is: ① callus approach: although a large amount of tissue culture plantlets can be obtained through callus in a short period of time, it has the weakness of easy variation; The foll

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0035] Example 1

[0036] (Take the tip of the top bud of the sugarcane as the explant)

[0037] (1) Selection and sterilization of explants: During April to May, take the top buds of sugar cane, peel off the older bracts, and treat them with hot water at 52°C for 30 minutes, then soak them in 75% alcohol 0.5~1.0min, and then treated with 0.1% mercury liters for 4min, rinse with sterile water 3~5 times;

[0038] (2) Basic medium: use MS medium, sucrose 30g / L, PH value 5.8~6.2;

[0039] (3) Start culture: under aseptic conditions, remove the leaf sheaths of the apical buds treated in step (1) carefully, pick 1~2mm stem tips, and inoculate them in MS+IBA0.02mg / L+activated carbon 1.0g / L Induce germination on the filter paper bridge culture medium;

[0040] (4) Differentiation culture: transfer the buds induced in step (3) to the differentiation medium to induce clump buds. The differentiation medium is MS medium, 6-BA0.5mg / L and IBA0.02mg / L;

[0041] (5) Strong seedling culture: Cut the ...

Example Embodiment

[0045] Example 2

[0046] (Take the stem tip of sugarcane axillary bud as explant)

[0047] (1) Selection and sterilization of explants: From July to October, cut the sugarcane into the stem segments of double buds → pretreat with 75% alcohol and prostaglandin → treat with hot water at 52°C for 30 minutes → set Accelerate the germination at high temperature in an artificial climate box (38℃, cultivate for 1 week), or place it in the fine river sand of the greenhouse for 1 week, take the germinated axillary buds, peel off the older bracts, and soak them in 75% alcohol for 0.5~1.0min , And then treated with 0.1% mercury liters for 3 minutes, rinse with sterile water 3~5 times;

[0048] (2) Basic medium: use MS medium, sucrose 30g / L, PH value 5.8~6.2;

[0049] (3) Start culture: The axillary buds treated in step (1) are carefully removed under aseptic conditions, and the leaf sheaths are carefully removed, and the apex of 1~2mm is picked and inoculated on filter paper of MS+IBA0.02mg / L...

Example Embodiment

[0055] Example 3

[0056] Test example (Virus detection)

[0057] Control material: diseased leaves, stems and roots of sugarcane collected from the field.

[0058] Processing material: The tissue culture plantlets obtained in Example 1 and Example 2 were used as materials.

[0059] The test results are shown in the following table:

[0060] deal with

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PUM

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Abstract

The invention relates to a method for rapidly breeding sugarcane stem tip by tissue culture and virus removal, belonging to the technical field of rapid breeding of plant by virus removal. The method comprises the following steps: carrying out disinfection pretreatment on a terminal bud of sugarcane and a cut double-bud stem section, with the terminal bud of the sugarcane and a stem tip tissue ofan axillary bud as explants, and carrying out stem tip initial culture, sprout differentiation culture, crowd sprout strong stock culture, plant root culture and virus checking by adopting a filter paper bridge culture way, thus massive virus-removed tissue culture seedlings are obtained. The invention has the advantages: the stem tip with the length of 1-2mm is taken as the explant, thus the processing operation is easy; the filter paper bridge culture way is adopted during the initial culture, thus the initiating speed is high and the perennial root dwarfing virus removing effect is thorough; and the stem tip is induced and germinated and is rapidly initiated and differentiates massive crowd sprouts, the month breeding coefficient reaches 5-8, and normomorph is realized in a long-term breeding process, thus the method is completely applicable to factory production on a large scale.

Description

technical field [0001] The invention relates to a method for detoxification and rapid propagation of sugarcane stem tips through tissue culture, in particular to a method for detoxification and rapid propagation of sugarcane stem tips through tissue culture, and belongs to the technical field of plant detoxification culture and rapid propagation. Background technique [0002] Sugarcane ratoon stunting disease (RSD) was first discovered in the sugarcane variety Q28 in Queensland, Australia from 1944 to 1945. Now it occurs widely in the main sugarcane producing areas in China and around the world, leading to the decline of species and seriously affecting The quality greatly shortens the economic service life of fine varieties. [0003] Sugarcane ratoon dwarf disease is caused by (Clavibacter xyli subsp.Xyli, Lxx), and the average infection rate of RSD cane plants is about 50%, which usually results in a 12-37% reduction in sugarcane yield, up to 60% in drought conditions, and ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 谭嘉娜陈月桂李奇伟杨俊贤吴文龙潘方胤
Owner 广州甘蔗糖业研究所湛江甘蔗研究中心
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