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High-fat chlorella pyrenoidosa culture solution and culture method thereof

A cultivation method and technology of chlorella, applied in the biological field, can solve the problems of low biomass of chlorella and limitation of photosynthesis of chlorella

Inactive Publication Date: 2011-06-15
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the technology of autotrophic culture of Chlorella has been mature, but there is a problem that light is blocked from entering the culture after the Chlorella cells are increased to a certain concentration, thus limiting the photosynthesis of Chlorella, resulting in the growth of the cultured Chlorella low biomass

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The activated chlorella was transferred to an Erlenmeyer flask containing a culture solution composed of 98% SE medium and 2% wood vinegar; the Erlenmeyer flask was placed on a shaking table with a rotation speed of 180 r / min for shaking culture. White fluorescent lamps were used for illumination, the light intensity was 6000Lx, and the light-to-dark ratio was 5:10h for interval illumination; the culture temperature was controlled at 21°C, and CO was continuously passed under the conditions of pH=6.6. 2 Cultivate for 21 days to obtain high-fat chlorella.

Embodiment 2

[0021] The activated chlorella was transferred to an Erlenmeyer flask containing a culture medium consisting of 97% SE medium and 3% wood vinegar; the Erlenmeyer flask was placed on a shaking table with a rotation speed of 150 r / min for shaking culture, White fluorescent lamps were used for illumination, the light intensity was 4000Lx, and the light-to-dark ratio was 4:8h for interval illumination. The culture temperature was controlled at 16°C, and CO was continuously passed under the condition of pH=5. 2 Cultivate for 13 days to obtain high-fat chlorella.

Embodiment 3

[0023] Transfer the activated chlorella to a Erlenmeyer flask containing a culture solution composed of 96% SE medium and 4% wood vinegar; place the Erlenmeyer flask on a shaking table with a rotation speed of 160r / min for shaking culture, and light Use white fluorescent lamps with a light intensity of 5000Lx and a light-to-dark ratio of 6:12h for interval lighting. The culture temperature was controlled at 19°C and the pH=5.8 was continuously passed through CO 2 Cultivate for 18 days to obtain high-fat chlorella.

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Abstract

The invention relates to a high-fat chlorella pyrenoidosa culture solution and a culture method thereof, belonging to the technical field of biology. The culture method comprises the following steps of: 1) transplanting activated chlorella pyrenoidosa into a conical flask filled with culture solution comprising 94-98% of SE medium and 2-6% of pyroligneous; 2) transferring the conical flask onto atable concentrator at the revolving speed of 150-180r / min for shaking and culturing; illuminating by a white fluorescent lamp; carrying out interval illumination at the light intensity of 4,000-6,000Lx and the lighting ration of 4-6:8-12h; and continuously introducing CO2 at the culture temperature of 15-21 DEG C for 12-25 days when the pH value of the culture solution is 5-6 to obtain the chlorella pyrenoidosa. The pyroligneoud which can accelerate alga to quickly grow to synthesize the fat is added into the culture solution. The culture method is simple to operate and has the advantage of low equipment investment and low cost, and excellent strain systems with high fat, high biomass and strong adaptability can be accurately, stably and efficiently cultured.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a high-fat chlorella culture solution and a culture method thereof. Background technique [0002] Microalgae biotechnology belongs to an important branch of life science research, and is currently a research hotspot and frontier at home and abroad. Chlorella in microalgae is a biological resource with important economic value and has great application and development potential. Chlorella is one of the earliest isolated and cultivated green algae. It belongs to the simplest photosynthetic organism with eukaryotic cells. It is rich in protein, unsaturated fatty acids, carotenoids, lutein, astaxanthin and various vitamins. , has extremely high nutritional value and the function of improving immunity. In addition, chlorella also contains a very important component - chlorella growth factor, which not only induces interferon, stimulates the functions of macrophages, T cells ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/28C12N1/12
Inventor 黄有军张启香闫道良胡君艳黄坚钦郑炳松夏国华陈国娟童森淼
Owner ZHEJIANG FORESTRY UNIVERSITY
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