Protein HlpB gene capable of stimulating plant to perform allergic reaction

An allergic reaction and protein technology, which is applied in the genetic field of biotechnology, can solve the problem of undiscovered disease-resistant immune response HlpB protein, etc.

Active Publication Date: 2011-06-15
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After searching the literature of the prior art, it was found that there is no report on the HlpB protein and the corresponding coding gene hlpB in the plant pathogenic Xanthomonas that can stimulate the plant to produce a disease-resistant immune response

Method used

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  • Protein HlpB gene capable of stimulating plant to perform allergic reaction
  • Protein HlpB gene capable of stimulating plant to perform allergic reaction
  • Protein HlpB gene capable of stimulating plant to perform allergic reaction

Examples

Experimental program
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Embodiment

[0018] 1. Construction of prokaryotic expression vector of plant pathogenic Xanthomonas hlpB gene

[0019] The present embodiment is obtained from rice stripe disease RS105 bacterial strain, rice bacterial blight PXO99 A The hlpB gene in bacterial strain, Brassica oleracea 85-10 strain, Canker sorrel citrus 306 strain and Pepper spot 8004 bacterial strain genome DNA was used as a template, and primers hlpB-F (SEQ ID NO.3) and hlpB-R (SEQ ID NO.3) were designed. .4), perform PCR amplification, and the PCR amplification conditions are: 94°C / 4min+(94°C / 30sec+55°C / 30sec+72°C / 30sec)×35 cycles+72°C / 10min. The final PCR product was connected to the pMD18-T vector, and the connected product was transformed into a competent cell DH5α, and the recombinant transformant pThhlpB with Amp resistance was screened. Recombinant transformants were digested with EcoR I and Sal I to recover the hlpB fragment, which was ligated into the expression vector pET41a(+) treated with the same enzyme dig...

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Abstract

The invention relates to a HlpB gene which belongs to the technical field of biology, comes from plant pathogenic xanthomonas campestris and can stimulate the plant to perform allergic reaction. The sequence of the gene is shown in SEQ ID NO.1 and is 552bp in length; the HlpB gene is coded by 182 amino acids; the sequence of the amino acids is shown in SEQ ID NO.2; the HlpB protein does not contain cysteine and contains rich glycine, the isoelectric point is 5.28, the molecular weight is 19.5kDa, the protein has thermal instability, is sensitive to protease and can stimulate tobacco to perform allergic reaction. The HlpB gene obtained in the invention can be used as the gene resource for the breeding for disease resistance of plant and the HlpB protein can be used as the protein drug to stimulate the plant to have disease resistance.

Description

technical field [0001] The invention relates to a gene in the field of biotechnology, in particular to a protein HlpB gene in plant pathogenic Xanthomonas that stimulates plants to produce allergic reactions. Background technique [0002] The resistance of plants to pathogen infection is manifested in two aspects: non-host and host resistance. The allergic response (Hypersensitive response, HR) of Gram-negative plant pathogenic bacteria on non-host plants is determined by certain elicitors in the pathogenic bacteria. This kind of HR is an anti-disease immune response, and the corresponding proteins that stimulate HR are called harpin-like proteins. The harpin protein types that have been identified from Gram-negative plant pathogenic bacteria include HrpZ, HrpN, HrpW, PopA1, Hpa1, HopAK1 and HopP1, and it is clear that only two stimulatory non-stimulating proteins, HrpW and Hpa1, exist in plant pathogenic Xanthomonas. Host plants produce HR harpin-like proteins. These har...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82
Inventor 陈功友邹华松邹丽芳车亦舟李玉蓉
Owner SHANGHAI JIAO TONG UNIV
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