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Method for preparing ovalbumin polypeptide by microwave-promoted enzymolysis

An ovalbumin and enzymatic hydrolysis technology is applied in the processing field of accelerated protein polypeptide hydrolysis, which can solve the problems of shortened reaction time, long relative cycle and high production cost, and achieves the effects of good product color, short reaction time and good quality.

Inactive Publication Date: 2011-06-15
陈栋梁
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation of ovalbumin polypeptide is usually prepared by enzymatic hydrolysis, which has a relatively long cycle and high production cost, and its application in food is limited
However, using microwave enzymolysis technology to prepare ovalbumin into ovalbumin polypeptide in a short period of time, the reaction time is shortened to ten minutes, compared with conventional enzymolysis or fermentation technology, which takes hours, ten hours or even days As far as the response cycle is concerned, the response time is greatly shortened, and this technology is still blank in the domestic market

Method used

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  • Method for preparing ovalbumin polypeptide by microwave-promoted enzymolysis
  • Method for preparing ovalbumin polypeptide by microwave-promoted enzymolysis

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Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1: the first step, weigh 1000g of ovalbumin, after pretreatment (remove egg yolk, eggshell), obtain ovalbumin; second step, after ovalbumin is homogenized, put into 5000ml microwave special reactor Add 2000ml of distilled water or pure water, stir well, adjust the pH value to 7.0 with potassium hydroxide, put it in the microwave equipment, turn on the microwave generator, power 100W, and the temperature rises to 50°C; the third step, press 1% of the weight of the egg white protein raw material, take the special enzyme for hydrolysis of egg white protein, add it to the obtained egg white protein aqueous solution, and turn on the microwave generator with a power of 100W, heat up to 50°C for enzymolysis, and microwave to accelerate the enzymolysis time Maintain for 30 minutes to obtain ovalbumin enzymatic hydrolyzate; in the fourth step, turn on the microwave generator 1000w and heat the obtained ovalbumin enzymatically to 100°C, keep it for 5 minutes to inactiva...

Embodiment 2

[0021] Embodiment 2: the first step, weigh 1500g of ovalbumin, after pretreatment (remove egg yolk, eggshell), obtain ovalbumin; second step, after ovalbumin is homogenized, put into 5000ml microwave special reactor Add 3000ml of distilled water or pure water, stir well, adjust the pH value to 7.5 with potassium hydroxide, put it in the microwave equipment, turn on the microwave generator, the power is 300W, and the temperature rises to 60°C; in the third step, press 1.5% of the weight of ovalbumin raw material, take the special enzyme for ovalbumin hydrolysis, add it to the obtained ovalbumin aqueous solution, and turn on the microwave generator with a power of 300W, heat up to 60°C for enzymolysis, and microwave to accelerate the enzymolysis time Maintain for 20 minutes to obtain ovalbumin enzymatic hydrolyzate; the fourth step is to turn on the microwave generator at 800w, microwave the obtained ovalbumin enzymatically to 100°C, keep it for 8 minutes to inactivate the enzyme...

Embodiment 3

[0022] Embodiment 3: the first step, weigh 2000g of ovalbumin, after pretreatment (remove egg yolk, eggshell), obtain ovalbumin; second step, after ovalbumin homogenate, put into 5000ml microwave special reactor Add 4000ml of distilled water or pure water, stir evenly, adjust the pH value to 8.0 with potassium hydroxide, put it in the microwave equipment, turn on the microwave generator, the power is 500W, and the temperature rises to 70°C; the third step, press 2.0% of the weight of ovalbumin raw material, take the special enzyme for ovalbumin hydrolysis, add it to the obtained ovalbumin aqueous solution, and turn on the microwave generator with a power of 500W, heat up to 70°C for enzymolysis, and microwave to accelerate the enzymolysis time Maintain for 15 minutes to obtain ovalbumin enzymatic hydrolyzate; in the fourth step, turn on the microwave generator at 500w and microwave the obtained ovalbumin enzymatically to 100°C, keep it for 10 minutes to inactivate the enzyme ac...

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Abstract

The invention discloses a method for preparing ovalbumin polypeptide by microwave-promoted hydrolysis of ovalbumin, which mainly fills in the blank of preparing the ovalbumin polypeptide from the ovalbumin in a short time in China. The ovalbumin polypeptide is prepared by a method of combining a microwave technology with a biological enzymolysis technology. By the method, a polypeptide product which has good appearance, good taste, strong functions, short preparation time and low production cost is developed. The method has the prominent advantages that: the ovalbumin polypeptide prepared by a microwave-promoted enzymolysis technology is superior to that prepared by the conventional enzymolysis technology; the obtained product does not contain impurities, has small molecular weight and is more easily absorbed and used by human bodies; and the process is simple, the reaction period is short, the enzymolysis degree is easy to control, and the product has high quality and good effects.

Description

technical field [0001] The invention relates to a processing technology for accelerating the hydrolysis of protein polypeptides, in particular to a method for preparing ovalbumin polypeptides by promoting the hydrolysis of ovalbumin with microwaves. Background technique [0002] As a high-quality protein recognized by the nutrition industry, egg white protein contains 8 kinds of amino acids necessary for the human body, and the proportion is balanced, and the utilization rate of the body is high. At the same time, because the nutrient composition and molecular structure of egg white protein are very similar to human plasma albumin, it is very nutritious and healthy, and it is also an ideal nutritional fortifier. Ovalbumin polypeptide is easily absorbed by the human body and has the effect of enhancing human immunity. Ovalbumin peptides are usually prepared by enzymatic hydrolysis, which has a relatively long cycle and high production costs, and its application in food is li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 陈栋梁
Owner 陈栋梁
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