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Method for culturing muscari botryoides mill with plant tissues

A technology for plant tissue culture and hyacinth, applied in the directions of botanical equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of low reproduction rate of grape hyacinth, unfavorable quality continuation and development, bulb atrophy, etc., and achieves easy automation. The effect of easy control, management and short growth cycle

Inactive Publication Date: 2012-02-08
江苏九久环境科技有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiencies in the prior art, to provide a method for cultivating grape hyacinths using plant tissue, which solves the problem that the grape hyacinth has a low reproductive rate and carries germs, which makes the varieties seriously degenerate and the bulbs shrink, which is not conducive to The problem of its quality continuation and development

Method used

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  • Method for culturing muscari botryoides mill with plant tissues

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Experimental program
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Effect test

Embodiment 1

[0026] Embodiment one: a kind of method adopting plant tissue culture grape hyacinth, comprises the following steps:

[0027] (1) Preparation of medium: MS medium was selected as the basic medium, and 2,4-D, IAA, NAA, 6-BA and IBA were used as regulated growth hormones to prepare five kinds of medium respectively: Medium A: MS+2 , 4-D0.5mg / L+6-BA 1.0mg / L, medium B: MS+NAA 0.5mg / L+6-BA 1.0mg / L, medium C: MS+IBA0.5mg / L+6 -BA 1.0mg / L, medium D: MS+IAA0.2mg / L, medium E: MS+IBA0.5mg / L; medium A, B, C and D were supplemented with 25g / L sucrose and 5g / L agar, add 25g / L sucrose and 2g / L agar in medium E, adjust the pH to 5.8 with 1mol / L NaOH solution and HCL solution, and mix culture medium A, B, C, D and E Sterilize in a high-pressure steam sterilizer at 121°C for 15 minutes, and the pressure in the high-pressure steam sterilizer is 0.1MPa;

[0028] (2) Disinfection of explants: choose grape hyacinth bulbs as explants, wash them with tap water for 1 hour, disinfect them with 1% 84...

Embodiment 2

[0035] Embodiment two: a kind of method adopting plant tissue culture grape hyacinth, comprises the following steps:

[0036] (1) Preparation of medium: MS medium was selected as the basic medium, and 2,4-D, IAA, NAA, 6-BA and IBA were used as regulated growth hormones to prepare five kinds of medium respectively: Medium A: MS+2 , 4-D1.0mg / L+6-BA 2.0mg / L, medium B: MS+NAA 1.0mg / L+6-BA 2.0mg / L, medium C: MS+IBA 1.0mg / L+6 -BA2.0mg / L, medium D: MS+IAA 1.0mg / L, medium E: MS+IBA1.0mg / L; medium A, B, C and D were supplemented with 30g / L sucrose and 5g / L agar, add 30g / L sucrose and 2g / L agar in medium E, adjust the pH to 6.0 with 2mol / L NaOH solution and HCL solution, and mix medium A, B, C, D and E Sterilize in a high-pressure steam sterilizer at 125°C for 20 minutes, and the pressure in the high-pressure steam sterilizer is 0.15MPa;

[0037] (2) explant disinfection: select the grape hyacinth bulb as the explant, after rinsing with tap water for 2h, disinfect it with 2% 84 disin...

Embodiment 3

[0044] Embodiment three: a kind of method adopting plant tissue culture grape hyacinth, comprises the following steps:

[0045] (1) Preparation of medium: MS medium was selected as the basic medium, and 2,4-D, IAA, NAA, 6-BA and IBA were used as regulated growth hormones to prepare five kinds of medium respectively: Medium A: MS+2 , 4-D0.6mg / L+6-BA 1.2mg / L, medium B: MS+NAA 0.6mg / L+6-BA 1.2mg / L, medium C: MS+IBA0.6mg / L+6 -BA 1.2mg / L, medium D: MS+IAA0.4mg / L, medium E: MS+IBA0.6mg / L; medium A, B, C and D were supplemented with 26g / L sucrose and 4.5 g / L agar, add 26g / L sucrose and 1.5g / L agar in medium E, adjust the pH to 5.9 with 1.5mol / L NaOH solution and HCL solution, and mix medium A, B, C, D and E are sterilized at 122°C for 16 minutes in a high-pressure steam sterilizer, and the pressure in the high-pressure steam sterilizer is 0.12MPa;

[0046] (2) Disinfection of explants: choose grape hyacinth bulbs as explants, wash them with tap water for 1.2 hours, disinfect them w...

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Abstract

The invention relates to a method for culturing muscari botryoides mill with plant tissues, comprising the following steps of: (1) preparing culture mediums: the culture medium A prepared from MS, 2, 4-D and 6-BA; the culture medium B prepared from MS, NAA and 6-BA; the culture medium C prepared from MS,IBA and 6-BA; the culture medium D prepared from MS and IAA; and theculture medium E prepared from MS and IBA; (2) disinfecting an explant; (3) inoculating the explant on the culture medium A for inducing culture to obtain a callus; (4) proliferation culture: transferring the callus to the culture medium B for culturing; (5) differentiating adventitious bud: transferring the callus which is subjected to propagation culture to the culture medium C to obtain the differentiated adventitious bud; (6) rooting culture: transferring the adventitious bud to the culture medium D for culturing to obtain a tissue cultured seedling; (7) subculturing: transferring the rooted tissue cultured seedling to the culture medium E; and (8) seedling adaptation: transplanting the tissue cultured seedling in a nutrition cup and then transplanting into a greenhouse. The method provided by the invention solves the problem that, since the muscari botryoides mill has low breading rate and carries virus, the breed of muscari botryoides mill degrades seriously and bulbs wither, which is bad for the continuation and development of the quality.

Description

technical field [0001] The invention relates to a method for cultivating grape hyacinth, in particular to a method for cultivating grape hyacinth by using plant tissue, and belongs to the technical field of plant tissue culture. Background technique [0002] Grape hyacinth (Muscari botryoides Mill) is a genus of blue pot flowers in the family Liliaceae, also known as blue pot flowers, grape lilies, and grape narcissus. Grape hyacinth is a perennial herbaceous bulbous flower, the plant is short, the leaves are linear-lanceolate, clustered, 10-30cm long, 0.5-1.5cm wide, spikes, 1-3 branches drawn from the leaf clusters, upright cylindrical , flower stems 10-30cm high, with 20-40 flowers. The flower colors are blue, blue-purple, white, light blue, light pink and yellow, beautiful and elegant. The flowering period is early, the natural flowering period is from mid-March to early April, the opening time is long, and the group flowering time can reach 20-30 days. In gardens, it...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 刘浩
Owner 江苏九久环境科技有限公司
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