Biosurfactant and polycyclic aromatic hydrocarbon-degrading strain enhanced compositing treatment of contaminated soil

A polycyclic aromatic hydrocarbon and biological surface technology, applied in the restoration of contaminated soil, etc., can solve the problems of long processing time, slow degradation of polycyclic aromatic hydrocarbons, and chemical pollution, so as to improve activity, reduce composting time, and speed up composting. The effect of the degradation process

Active Publication Date: 2011-07-20
HONG KONG BAPTIST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] In order to overcome the problems that existing polycyclic aromatic hydrocarbon-contaminated soil composting treatment technology has a slow degradation rate of macromolecular polycyclic aromatic hydrocarbons containing five or more benzene rings, a long processing time, and the introduction of new chemical pollution during the treatment process, the present invention Provides an ex-situ bioremediation technology for polycyclic aromatic hydrocarbon-contaminated soil, which uses organic matter, biosurfactant or its production bacteria, and polycyclic aromatic hydrocarbon-degrading bacteria to promote composting treatment of polycyclic aromatic hydrocarbon pollutant-contaminated soil restoration in polluted soil Technology

Method used

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  • Biosurfactant and polycyclic aromatic hydrocarbon-degrading strain enhanced compositing treatment of contaminated soil
  • Biosurfactant and polycyclic aromatic hydrocarbon-degrading strain enhanced compositing treatment of contaminated soil
  • Biosurfactant and polycyclic aromatic hydrocarbon-degrading strain enhanced compositing treatment of contaminated soil

Examples

Experimental program
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Effect test

Embodiment 1

[0057] Embodiment 1: Enrichment and cultivation of biosurfactant production strains from soil

[0058] Soil samples were collected from the crude oil-contaminated soil around the abandoned oil wells in the Tianjin Dagang Oilfield Oil Production Plant. In the laboratory, 5 grams of soil samples were taken and suspended in 95 milliliters of inorganic salt-naphthalene medium. The culture was cultured on a shaker at 50-70 degrees Celsius for 7-14 days, then 10 ml of culture solution was taken to inoculate the same fresh medium, and after continuous acclimatization for 3-5 times under the same culture conditions, a single colony was isolated by streaking on an agar plate and cultured. Cultivated in an incubator. After forming a single colony, observe the colony morphology and cell microscopic morphology to distinguish different strains. The isolated strains were respectively inoculated in broth culture medium and cultured on a shaker at 50-70 degrees Celsius. The surface tension ...

Embodiment 2

[0059] Embodiment 2: the separation and preparation method of biosurfactant

[0060] The biosurfactant was extracted from the culture solution of surfactant-producing bacteria. The extraction method is as follows: the surfactant-producing bacteria strain is centrifuged to separate the cells, and the cell-free supernatant is collected for extraction with n-hexane. The combined emulsified phases were collected and rotary evaporated at 40-70 degrees Celsius, then freeze-dried at minus 20 degrees Celsius, and then dissolved in distilled water to prepare a biosurfactant solution. Crude biosurfactant can be obtained through this extraction step.

Embodiment 3

[0061] Example 3: Enrichment culture of polycyclic aromatic hydrocarbon degrading bacterial community and polycyclic aromatic hydrocarbon degrading bacterial strains

[0062] The present invention can use a variety of polycyclic aromatic hydrocarbon degrading bacteria, including degrading bacterial communities and isolated and purified degrading bacterial strains to enhance bioremediation of polluted soil, which are described in detail below. The enrichment culture of polycyclic aromatic hydrocarbon degrading bacterial community includes: collecting soil samples polluted by polycyclic aromatic hydrocarbons, such as the soil near the former site of ship repair yard in Tsing Yi, Hong Kong. Take 5 grams of soil samples and suspend them in 95 milliliters of inorganic salts-naphthalene medium. The culture is cultured on a shaker at 50-70 degrees Celsius for 7-14 days, and then 10 ml of the culture solution is taken to inoculate the same fresh medium. After continuous acclimatizati...

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Abstract

The invention relates to a biosurfactant and a technology for remediation of contaminated soil through polycyclic aromatic hydrocarbon-degrading strain enhanced compositing treatment. Biosurfactant-producing bacteria produce the biosurfactant; the biosurfactant is extracted and separated for soil remediation; and in the remediation process, soil contaminated polycyclic aromatic hydrocarbons is uniformly mixed with organic matters to improve the organic matter content of the soil so as to facilitate composting treatment, the moisture content of the mixture is regulated, polycyclic aromatic hydrocarbon-degrading strains are inoculated in the composting material, and the biosurfactant-producing bacteria are inoculated into the composting material, or the surfactant produced by the producing bacteria is added into the composting material to improve the bioavailability of the polycyclic aromatic hydrocarbons, the removal rate of micromolecule polycyclic aromatic hydrocarbons (phenanthrene) is 100 percent after the soil is treated for 42 days, the speed of degrading macromolecular polycyclic aromatic hydrocarbons (benzo(a)pyrene) in the treatment process is improved by 392 percent, and 4.9 milligrams of macromolecular polycyclic aromatic hydrocarbons in each kilogram of soil are decomposed per day; therefore, the technology is farther superior to other soil remediation technologies.

Description

1. Technical field [0001] The invention belongs to the ectopic bioremediation technology of polluted soil, and is a technology for promoting composting treatment of polycyclic aromatic hydrocarbon pollutants polluted soil by adding organic matter, biosurfactant or its production bacteria and polycyclic aromatic hydrocarbon degrading bacteria to polluted soil . 2. Background technology [0002] Polycyclic aromatic hydrocarbons (PAHs) refer to condensed ring compounds containing two or more benzene rings, and are a class of pollutants widely present in soil. Polycyclic aromatic hydrocarbons have poor water solubility, stable chemical properties, and strong carcinogenic, mutagenic, and teratogenic effects. How to effectively remove soil pollution caused by polycyclic aromatic hydrocarbons has long been a worldwide research hotspot and problem. At present, although there are many studies on the degradation of PAHs, all of them have the disadvantages of low degradation efficienc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/08
Inventor 黄焕忠赵震勇
Owner HONG KONG BAPTIST UNIV
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