Agent and method for rapid onsite detection of melamine

Abstract

The invention discloses an agent and method for the rapid onsite detection of melamine. The method comprises the steps of: adding AlCl3, ZnCl2 or MgCl2 to a 20-25% trichloroacetic acid aqueous solution to obtain an extracting agent the ion concentration of which is enabled to be 10 to 30mmol / L; preparing a saturated aqueous solution of CuCl2 as an indicator; sufficiently mixing 4 to 5 parts by volume of liquid sample with 1 part by volume of the extracting agent to obtain an extracting solution, and removing precipitates in the extracting solution to obtain a solution to be detected; adding 1 part by volume of the indicator to 20 parts of the solution to be detected and uniformly mixing, and observing precipitate generation condition. Compared with the existing detection methods conforming to national standard, the method of the invention has the detection principle different from chromatographic analysis detection, is convenient and fast and shortens the detection time to 10 to 20 minutes, and the detection method has the lower limit not more than 30mg / kg, meets the demand of general detection, can be used as an auxiliary mean for national standard detection, and has wide range of detection objects.

Description

technical field [0001] The invention relates to a rapid qualitative detection technology for melamine in food and feed safety monitoring, in particular to a rapid on-site detection reagent and detection method for melamine in milk powder, protein powder, animal feed and liquid dairy products. Background technique [0002] Melamine (Mel) is a widely used organic chemical raw material with nitrogen heterocyclic structure, because of its structure not only has nitrogen heterocyclic ring but also contains multiple amino nitrogens, making its nitrogen content up to more than 66%, so Mel is used by some Unscrupulous traders add it to the product to inflate the protein content of the product. The abnormal deaths of cats and dogs in the United States in 2007 and the milk powder incident in China in 2008 were all caused by this. [0003] At present, the main methods for detecting Mel are high-pressure liquid chromatography, liquid chromatography-mass spectrometry, and gas spectromet...

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Problems solved by technology

[0003] At present, the main methods for detecting Mel are high-pressure liquid chromatography, liquid chromatography-mass spectrometry, and gas spectrometry, which not only have high requirements on the quality of instruments, operators, and laboratories, but also require time-consuming detection and high operating costs, and are difficult to meet modern On-site food safety monitoring requires simple, fast and portable detection methods

[0004] Since the country collected the Mel rapid monitoring method in 2008, dozens of Mel detection patent technologies have come out, most of which are ELISA kits developed using immunological principles, which have strong detection specificity and relatively high sensitivity, but the kit preparation It involves complex procedures such as the preparation of complete Mel antigens, animal immunization, and cross-linking of Mel and enzymes, and the cost of reagents is high.

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