Method for producing alcohol by utilizing cassava as a raw material through applying enzyme preparation production fermenting waste liquid

A technology of fermentation waste liquid and enzyme preparation, applied in the field of fermentation, can solve the problems of increased production cost, pollution of the surrounding environment, low component content, etc., and achieve the effects of large profits, reduced production costs, and waste liquid treatment costs

Inactive Publication Date: 2011-08-10
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the relatively low content of various components of the above-mentioned waste liquid, it is of little value to be recycled again. Some small and medium-sized enterprises directly discharge these waste liquids, polluting the surrounding environment
Some large enterprises treat these waste liquids in accordance with the national sewage discharge standards, resulting in a substantial increase in production costs

Method used

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  • Method for producing alcohol by utilizing cassava as a raw material through applying enzyme preparation production fermenting waste liquid
  • Method for producing alcohol by utilizing cassava as a raw material through applying enzyme preparation production fermenting waste liquid
  • Method for producing alcohol by utilizing cassava as a raw material through applying enzyme preparation production fermenting waste liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 The effect of adding saccharification enzyme fermentation waste liquid on the yield of alcohol

[0024] Enzyme preparation production fermentation waste liquid is applied to cassava raw materials to produce alcohol, including cassava flour slurry, liquefaction, saccharification and fermentation, the steps are as follows:

[0025] The cassava is crushed and passed through the bottom of a sieve with an aperture of 1.8 mm to obtain cassava flour, and then 1 kg of cassava flour is taken, and the cassava flour is mixed with 2.6L of water in a weight-to-volume ratio of 1:2.6, and stirred evenly to obtain a cassava flour slurry; Add 0.04L of amylase diluent (from 20,000 U / mL diluent to 200U / mL) to the powder slurry, mix well, and control the liquefaction at 96°C for 80 minutes to obtain liquefied cassava pulp;

[0026] Cool the liquefied cassava pulp to 60°C, add 0.088L glucoamylase diluent (diluted from 100,000 U / mL to 1000U / mL) and 0.15L glucoamylase fermentation waste li...

Embodiment 2

[0036] Example 2 The effect of adding amylase fermentation waste liquid alone on alcohol production

[0037] The enzyme preparation production fermentation waste liquid described in Example 1 is applied to the method for producing alcohol from cassava raw materials. The difference is that only 0.1L of amylase fermentation waste liquid is added to the cassava pulp during the liquefaction process; Add 0.1L of glucoamylase diluent to the liquefied cassava pulp at 60°C, without adding glucoamylase fermentation waste liquid.

[0038] At the same time, do comparative experiments, as shown in the following table:

[0039]

Group No

Raw material quantity (kg)

+

Water (L)

starch

Enzyme

dilution

Liquid (L)

Amylase hair

yeast

Waste liquid (L)

Glucoamylase

Diluent

(L)

Nutrients

(g)

Activated yeast

/ L

Control experiment

1+2.6

0.04

0

0.10

1

0.02

Add alpha-amylase

Fermentation waste liquid experiment

...

Embodiment 3

[0045] Example 3 The effect of simultaneous addition of α-amylase fermentation waste liquid and saccharification enzyme fermentation waste liquid on alcohol yield

[0046] The enzyme preparation production fermentation waste liquid as described in Example 1 is applied to the method for producing alcohol from cassava raw materials. The difference is that 0.05L amylase fermentation waste liquid and 0.02L amylase diluent are added to the cassava pulp during the liquefaction process. In the saccharification process, 0.092L of diluent of saccharification enzyme and 0.1L of saccharification enzyme fermentation waste liquid are added to the liquefied cassava pulp cooled to 60°C, 0.5g of nutrient salt is added in the process of saccharification, and 0.02L of activated Saccharomyces cerevisiae is added.

[0047] At the same time do a comparative test, as shown in the following table:

[0048]

Group No

Raw material quantity (kg)+

Water (L)

Amylase thin

Release (L)

Amylase ...

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PUM

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Abstract

The invention relates to a method for producing alcohol by utilizing cassava as a raw material and adding the production fermenting waste liquid of amylase and diastatic enzyme, which belongs to the technical field of fermentation. The method for producing alcohol by utilizing cassava as a raw material through applying enzyme preparation production fermenting waste liquid comprises the steps of cassava powder slurrying, liquefaction, saccharification and fermentation. The method is characterized by singly adding amylase fermenting waste liquid in the liquefying process without adding amylase or simultaneously adding amylase fermenting waste liquid when adding amylase and/or singly adding diastatic enzyme fermenting waste liquid in the saccharifying process without adding diastatic enzyme or simultaneously adding diastatic enzyme fermenting waste liquid when adding diastatic enzyme. Practical production indicates that the method can improve the alcohol yield of cassava raw materials, reduce the amylase consumption, diastatic enzyme consumption, nutritive salt consumption and the waste liquid treatment cost of enzyme preparation production and improves production benefits of alcohol.

Description

Technical field [0001] The invention relates to a method for producing fermentation waste liquid of enzyme preparations for alcohol production, in particular to a method for producing alcohol using cassava as a raw material and adding amylase and glucoamylase to produce fermentation waste liquid, which belongs to the technical field of fermentation. Background technique [0002] At present, amylase and glucoamylase are widely used in other industries such as alcohol, liquor, beer, brewing, monosodium glutamate, starch sugar, fermentation industry, etc. Amylase and glucoamylase preparations are produced on a large scale while producing a large amount of amylase fermentation waste liquid and Glucoamylase fermentation waste liquid. After testing, the COD of these waste liquids is about 50000 mg / L, among which amylase waste liquid contains amylase 100-120 U / mL, and glucoamylase waste liquid contains glucoamylase (80-100) U / mL. The waste liquid contains reducing sugar (0.9-1.0) g / mL....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/14C12R1/865
Inventor 王君高王乐高晓娟
Owner QILU UNIV OF TECH
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