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Method for producing alcohol by applying enzyme preparation production fermenting waste liquid to raw material of cassava

A technology for fermentation waste liquid and enzyme preparation, applied in the field of fermentation, can solve the problems of increasing production cost, polluting the surrounding environment, low component content, etc., and achieve the effects of large profits, saving waste liquid treatment costs, and reducing production costs.

Inactive Publication Date: 2013-04-10
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the relatively low content of various components of the above-mentioned waste liquid, it is of little value to be recycled again. Some small and medium-sized enterprises directly discharge these waste liquids, polluting the surrounding environment
Some large enterprises treat these waste liquids in accordance with the national sewage discharge standards, resulting in a substantial increase in production costs

Method used

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  • Method for producing alcohol by applying enzyme preparation production fermenting waste liquid to raw material of cassava
  • Method for producing alcohol by applying enzyme preparation production fermenting waste liquid to raw material of cassava
  • Method for producing alcohol by applying enzyme preparation production fermenting waste liquid to raw material of cassava

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 Adding glucoamylase fermentation waste liquid alone to the influence of alcohol production

[0024] Enzyme preparation production fermentation waste liquid is applied to the method for producing alcohol from cassava raw materials, including cassava flour slurry, liquefaction, saccharification and fermentation, and the steps are as follows:

[0025] The cassava is pulverized and passed through a sieve with an aperture of 1.8mm to obtain cassava flour, then take 1kg of cassava flour, mix the cassava flour with 2.6L of water according to the ratio of weight to volume ratio 1: 2.6, and stir evenly to obtain cassava flour slurry; Add 0.04L diluent of amylase (from 20,000 U / mL to 200 U / mL) into the slurry, mix well, and liquefy at 96°C for 80 minutes to obtain liquefied cassava pulp;

[0026] Cool the liquefied cassava pulp to 60°C, add 0.088L glucoamylase diluent (diluted from 100,000 U / mL to 1000U / mL) and 0.15L glucoamylase fermentation waste liquid, mix well, ...

Embodiment 2

[0036] Embodiment 2 separately adds the impact of amylase fermentation waste liquid on alcohol production

[0037] Enzyme preparation production fermentation waste liquid as described in embodiment 1 is applied to the method for producing ethanol from cassava raw material, and the difference is that only 0.1L amylase fermentation waste liquid is added to cassava pulp in the liquefaction process; Add 0.1L saccharification enzyme diluent to the liquefied cassava pulp at 60°C, without adding saccharification enzyme fermentation waste liquid.

[0038] At the same time, do comparative experiments, as shown in the table below:

[0039]

Group No

Raw material quantity (kg)

+

water (L)

starch

enzyme

to dilute

Liquid (L)

amylase hair

leaven

Waste liquid (L)

Glucoamylase

Diluent

(L)

Nutrients

(g)

...

Embodiment 3

[0045] Example 3 Effects of Simultaneously Adding α-amylase Fermentation Waste Liquid and Glucoamylase Fermentation Waste Liquid on Alcohol Production

[0046]Enzyme preparation production fermentation waste liquid as described in embodiment 1 is applied to the method for cassava raw material production alcohol, and difference is, in cassava slurry, add 0.05L amylase fermentation waste liquid and 0.02L amylase dilution in the liquefaction process, During the saccharification process, add 0.092L glucoamylase dilution and 0.1L glucoamylase fermentation waste liquid to the liquefied cassava pulp cooled to 60°C, add 0.5g nutrient salt, and add 0.02L activated Saccharomyces cerevisiae during the saccharification process.

[0047] At the same time, do a comparative test, as shown in the table below:

[0048]

Group No

Amount of raw material(kg)+

water (L)

dilute amylase

Release solution (L)

amylase hair

leaven

Wa...

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Abstract

The invention relates to a method for producing alcohol by utilizing cassava as a raw material and adding the production fermenting waste liquid of amylase and diastatic enzyme, which belongs to the technical field of fermentation. The method for producing alcohol by utilizing cassava as a raw material through applying enzyme preparation production fermenting waste liquid comprises the steps of cassava powder slurrying, liquefaction, saccharification and fermentation. The method is characterized by singly adding amylase fermenting waste liquid in the liquefying process without adding amylase or simultaneously adding amylase fermenting waste liquid when adding amylase and / or singly adding diastatic enzyme fermenting waste liquid in the saccharifying process without adding diastatic enzyme or simultaneously adding diastatic enzyme fermenting waste liquid when adding diastatic enzyme. Practical production indicates that the method can improve the alcohol yield of cassava raw materials, reduce the amylase consumption, diastatic enzyme consumption, nutritive salt consumption and the waste liquid treatment cost of enzyme preparation production and improves production benefits of alcohol.

Description

technical field [0001] The invention relates to a method for producing alcohol by using fermentation waste liquid produced by enzyme preparations, in particular to a method for producing alcohol by using cassava as a raw material and adding amylase and glucoamylase to the production fermentation waste liquid, which belongs to the field of fermentation technology. Background technique [0002] At present, amylase and glucoamylase are widely used in other industries such as alcohol, liquor, beer, brewing, monosodium glutamate, starch sugar, fermentation industry, etc. Amylase and glucoamylase preparations produce a large amount of amylase fermentation waste liquid and Glucoamylase fermentation waste liquid. After testing, the COD of these waste liquids is about 50000 mg / L, among which the amylase waste liquid contains 100-120 U / mL of amylase, and the glucoamylase waste liquid contains 80-100 U / mL of glucoamylase. Waste liquid contains reducing sugar (0.9-1.0) g / mL. In additi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/14C12R1/865
Inventor 王君高王乐高晓娟
Owner QILU UNIV OF TECH
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