Nucleic acid detection kit for 12+2 high-risk human papilloma virus (HPV)

A papilloma virus and detection kit technology, applied in the field of genes, can solve the problems of high cost, complicated operation requirements, and inability to distinguish HPV types.
CN102154524BActive Publication Date: 2012-11-28潮州凯普生物化学有限公司 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
潮州凯普生物化学有限公司
Publication Date
2012-11-28

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Abstract

The invention discloses a nucleic acid detection kit for 12+2 high-risk human papilloma virus (HPV), comprising: (1) probes as shown in SEQ ID No:1-12 and primers as shown in SEQ ID No: 13-36 of a nucleotide sequence which is only complementary with the DNAs of 12 high-risk HPV; (2) a probe as shown in SEQ ID No:37 and primers as shown in SEQ ID No: 38-39 of a nucleotide sequence which is only complementary with the DNA of high-risk HPV16; (3) a probe as shown in SEQ ID No:40 and primers as shown in SEQ ID No: 41-42 of a nucleotide sequence which is only complementary with the DNA of high-risk HPV 18; and (4) a probe as shown in SEQ ID No: 43 and primers as shown in SEQ ID No: 44-45 of a nucleotide sequence which is only complementary with the DNA of an intracellular beta-globin gene, wherein each group of probes respectively mark different dyes with the maximum emission wavelengths of 518nm, 538-553nm, 574-575nm, 607-615nm, 640nm, 666-667nm and 690nm. The kit disclosed by the invention can furthest lighten huge cost brought by HPV gene detection in China, and the health of people is protected.
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Description

Technical field

[0001] The invention relates to the field of genes, in particular to a 12+2 high-risk human papilloma virus nucleic acid detection kit. Background technique

[0002] Human papillomavirus (HPV) has attracted people's attention since it was discovered to be associated with cervical cancer in the late 1970s and early 1980s. A large number of research results indicate that HPV has the most important role in the pathology of cervical cell abnormalities and cervical cancer. Currently, more than 100 HPV genotypes have been identified, of which about 40 are related to reproductive tract infections. The high-risk type is mainly related to the occurrence of cervical intraepithelial neoplasia (cervical intraepithelial neoplasia, CIN) II, III and cervical cancer. The low-risk type is generally not related to malignant lesions, and mainly causes condyloma acuminatum and low-grade CIN (CINI).

[0003] Cervical cancer is one of the common gynecological malignancies, and its inc...

Claims

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