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Fetor pseudomonas putida ZJB09102 and application thereof in preparation of epoxy chloropropane

A technology of ZJB09102, Pseudomonas putida, applied in the direction of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems that epichlorohydrin has not been reported in the literature

Active Publication Date: 2012-11-14
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature report on the production of epichlorohydrin by dehalogenation by biological method using 1,3-dichloro-2-propanol as substrate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Medium formula (w / v): 1,3-dichloro-2-propanol: 0.5%, yeast powder: 0.1%, peptone: 0.1%, (NH 4 ) 2 SO 4 : 0.1%, NaH 2 PO 4 2H 2 O: 0.1%, MgSO 4 : 0.01%, prepared with tap water, adjusted the pH to 5.0 with HCl solution, sterilized for later use.

[0024] Take 100mL of the above-mentioned culture medium, divide it into two 250mL Erlenmeyer flasks, sterilize, insert Pseudomonas putida ZJB09102 (CCTCC No: M 2010273), and cultivate the bacteria, shaker speed 150r / min, at 28°C Cultivate on a shaker for 24 hours as the seed solution.

[0025] Take 2L of the above culture medium, divide it into 40 500mL shake flasks, and sterilize. Insert the seed solution, insert the seed solution with an inoculation amount of 1.5% by volume, and carry out cultivation. The cultivation temperature is 20°C, the shaking table speed is 150r / min, and the cultivation time is 72h. 1,3-dichloro-2-propanol The conversion to epichlorohydrin was 88%.

[0026] 1.5 L of the above-mentioned ferment...

Embodiment 2

[0028] Medium formula: 1,3-dichloro-2-propanol: 0.2%, yeast powder: 2.5%, peptone: 0.5%, (NH 4 ) 2 SO 4 : 0.1%, NaH 2 PO 4 2H 2 O: 0.5%, MgSO 4 : 0.05%, prepared with tap water, adjusted the pH to 7.0 with NaOH solution, sterilized for later use.

[0029] Take 500mL culture medium, divide it evenly into ten 500mL Erlenmeyer flasks, sterilize it, insert Pseudomonas putida ZJB09102, and cultivate it. The culture temperature is 28°C, and the culture time is 50h. Carried out under the hood, the shaking table rotates at a speed of 200r / min. The conversion rate of 1,3-dichloro-2-propanol to epichlorohydrin was 90%.

[0030] 400 mL of the above-mentioned fermentation broth was collected, separated and purified, and the steps were the same as in Example 1 to obtain 0.52 g of epichlorohydrin.

Embodiment 3

[0032]Fermentation medium formula: 1,3-dichloro-2-propanol: 2%, yeast powder: 2.5%, peptone: 2.5%, (NH 4 ) 2 SO 4 : 2.5%, NaH 2 PO 4 2H 2 O: 0.1%, MgSO 4 : 0.05%, prepared with tap water, adjusted the pH to 7.0 with NaOH solution, sterilized for later use.

[0033] Seed medium formula: yeast powder: 2.5%, peptone: 2.5%, (NH 4 ) 2 SO 4 : 2.5%, NaH 2 PO 4 2H 2 O: 0.1%, MgSO 4 : 0.05%, prepared with tap water, adjusted the pH to 7.0 with NaOH solution, and sterilized for later use.

[0034] Take 100mL seed culture medium, divide it evenly into two 500mL Erlenmeyer flasks, sterilize, insert Pseudomonas putida ZJB09102, cultivate the bacteria, shake the table at a speed of 200r / min, and cultivate it at 30°C for 24 hours as the seed solution ,spare.

[0035] Take 4L of fermentation medium, divide it equally into 80 500mL Erlenmeyer flasks, and sterilize. Insert the seed solution, insert the seed solution with an inoculation volume of 2.0% by volume, and cultivate at a...

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PUM

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Abstract

The invention provides fetor pseudomonas putida ZJB09102, which can catalyze dehalogenation reaction, and application thereof in preparation of epoxy chloropropane. The strain, namely the fetor pseudomonas putida ZJB09102 is collected in China Center for Type Culture Collection the address of which is Wuhan University, Wuhan, Chain, 430072; the collection number of the strain is CCTCC No: M2010273; and the collection data of the strain is October 24, 2010. The pseudomonas putida ZJB09102 mainly has the benefits as follows: the microbial strain, namely the fetor pseudomonas putida ZJB09102, has very strong dehalogenation reaction capability; when the strain is utilized for producing the epoxy chloropropane, the conversion rate of 1,3-dichloro-2-propanol achieves 50%-90% (that is, 1 mole ofthe 1,3-dichloro-2-propanol can be converted to 0.50-0.90 mole of the epoxy chloropropane); and furthermore, in the production process, a chemical catalyst is not used, therefore, the strain has the characteristics of being green and environment-friendly and is applicable to the industrialized production of the epoxy chloropropane.

Description

(1) Technical field [0001] The invention relates to a strain of Pseudomonas putida ZJB09102 capable of catalyzing dehalogenation reaction and its application in preparing epichlorohydrin. (2) Background technology [0002] Epichlorohydrin is a colorless, transparent, oily liquid with a pungent odor similar to chloroform and ether. It is a volatile and unstable liquid with a boiling point of 117.9°C and slightly soluble in water (the solubility at 20°C is 7.04g), easily soluble in alcohol, ether, benzene and other organic solvents, can form azeotropes with various organic liquids, and is toxic and narcotic. It is the main raw material for the production of epoxy resin, chlorohydrin rubber, nitrated explosives, glass fiber reinforced plastics, and electrical insulation products; it can also be used for the production of adhesives, cation exchange resins, etc., as plasticizers, stabilizers, surfactants, medicines And solvents for cellulose esters, cellulose ethers and resins, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P17/02C12R1/40
Inventor 郑裕国胡忠策许辉辉
Owner ZHEJIANG UNIV OF TECH
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