Nanometer material with biosensing function and preparation method thereof
A nanomaterial and biosensing technology, which is applied in the field of nanomaterials with biosensing function and its preparation, can solve the problems that the application of infinite coordination polymers has not been reported yet, and achieve the advantages of miniaturization, accelerated electron transfer, The effect of shortening the distance
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[0025] Embodiment 1, preparation has the nanometer material (MG / GDH / Tb-NAD of biosensing function + ) and electrochemical sensors
[0026] MG (methylene green), GDH (glucose dehydrogenase) and NAD + At the same time, they were dissolved in 1 mL of HEPES buffer solution (pH 7.4, concentration 0.1M) so that their respective concentrations were 0.1 mM, 200 μg / mL and 10 mM. At room temperature, 1 mL of Tb(NO 3 ) 3 ·6H 2 O (10 mM) aqueous solution was added to the above solution and allowed to stand for several hours. The resulting precipitate was washed with pure water and centrifuged, and the supernatant was discarded. Repeat the above operation at least three times, and finally disperse with pure water. Then it was characterized by SEM (see figure 1 ), it can be seen that the size of the obtained material is nanoscale.
[0027] MG / GDH / Tb-NAD + Electrochemical Sensing Research for:
[0028] 5 μL MG / GDH / Tb-NAD + The water-dispersed liquid was drop-coated on the surface ...
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