Nanometer material with biosensing function and preparation method thereof

A nanomaterial and biosensing technology, which is applied in the field of nanomaterials with biosensing function and its preparation, can solve the problems that the application of infinite coordination polymers has not been reported yet, and achieve the advantages of miniaturization, accelerated electron transfer, The effect of shortening the distance

Active Publication Date: 2011-09-07
INST OF CHEM CHINESE ACAD OF SCI
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Problems solved by technology

However, to date, the electrochemical applications of

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  • Nanometer material with biosensing function and preparation method thereof
  • Nanometer material with biosensing function and preparation method thereof

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Experimental program
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Embodiment 1

[0025] Embodiment 1, preparation has the nanometer material (MG / GDH / Tb-NAD of biosensing function + ) and electrochemical sensors

[0026] MG (methylene green), GDH (glucose dehydrogenase) and NAD + At the same time, they were dissolved in 1 mL of HEPES buffer solution (pH 7.4, concentration 0.1M) so that their respective concentrations were 0.1 mM, 200 μg / mL and 10 mM. At room temperature, 1 mL of Tb(NO 3 ) 3 ·6H 2 O (10 mM) aqueous solution was added to the above solution and allowed to stand for several hours. The resulting precipitate was washed with pure water and centrifuged, and the supernatant was discarded. Repeat the above operation at least three times, and finally disperse with pure water. Then it was characterized by SEM (see figure 1 ), it can be seen that the size of the obtained material is nanoscale.

[0027] MG / GDH / Tb-NAD + Electrochemical Sensing Research for:

[0028] 5 μL MG / GDH / Tb-NAD + The water-dispersed liquid was drop-coated on the surface ...

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Abstract

The invention discloses a one-step synthetized nanometer material with a biosensing function and applications thereof on the biosensing aspect. The nanometer material is prepared according to the method comprising the following steps of: at room temperature, mixing = enzyme, coenzyme and an electron transfer vector, and then adding earth ions in the mixed liquor to obtain the nanometer particles integrating all the active components. The nanometer particles are modified to the surface of the glassy carbon electrode, and the biosensing performance of the nanometer particles is determined by using the electrochemistry method. The uniform nanometer material with the biosensing function can be prepared by using conventional reactants through a one-step synthesis process, and the prepared nanometer material has excellent biosensing performance.

Description

technical field [0001] The invention belongs to the electrochemical application field of biosensing materials, and in particular relates to a nanometer material with a biosensing function and a preparation method and application thereof. Background technique [0002] Electrochemical biosensors have attracted extensive attention in recent decades. This is because, compared with other sensors, electrochemical biosensors have both the simplicity of electrochemical sensing and the specific recognition of molecules by biosensing, so they have outstanding advantages such as good selectivity, high sensitivity, simplicity, and portability. , has been widely used in public security, medical and health and environmental monitoring. Generally speaking, bioelectrochemical sensors include three parts: electron transfer mediators, enzymes, and coenzymes (biosensing based on dehydrogenase systems). In the preparation process of traditional bioelectrochemical sensors, electron transfer me...

Claims

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Application Information

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IPC IPC(8): B82Y15/00B82Y40/00G01N27/30
Inventor 毛兰群杨丽芬黄鹏程
Owner INST OF CHEM CHINESE ACAD OF SCI
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