Peristaltic circulating bioreactor and culture solution circulating method thereof

Abstract

The invention relates to a peristaltic circulating bioreactor and a culture solution circulating method thereof. In the culture solution circulating method, a container body of the bioreactor is provided with more than one pair of upper ports and lower ports communicated with an inner cavity of the container body; the upper ports are communicated with the lower ports through soft peristaltic pipes and communicating pipes; the joints of the peristaltic pipes and the communicating pipes are higher than the horizontal surfaces of the upper ports; the peristaltic pipes downwards extend below the horizontal surfaces of the upper ports; culture solution in the container body enters the peristaltic pipes from the upper ports; the peristaltic pipes are driven upwards below the peristaltic pipes or outwards above the peristaltic pipes at positions which are close to the upper ports to be lifted above the liquid surface of the culture solution; the culture solution in the peristaltic pipes flows into the communicating pipes and flows back to the container body through the lower ports; the peristaltic pipes are put down until the pipe sections of the peristaltic pipes, which are close to the upper ports, are positioned on the liquid surface of the culture solution; and the culture solution on upper layers flows into the peristaltic pipes again, and the peristaltic pipes are driven upwards below the peristaltic pipes or outwards above the peristaltic pipes at the positions which are close to the upper ports repeatedly, so that the culture solution is circulated.

Description

technical field [0001] The invention relates to a peristaltic circulation bioreactor, the bioreactor is mainly used for animal cell culture and the production of biotechnology products using animal cells, and can also be used for the cultivation of insect cells, plant cells and microorganisms and their biotechnology products production. Simultaneously, the invention also relates to a method for circulating culture fluid of a bioreactor. Background technique [0002] The use of cultured animal cells (including human cells) for life science research, cell transplantation to treat clinical diseases and the production of biotechnology products (such as proteins, polypeptides, nucleic acids, antibodies, viral vectors and viral vaccines) often requires For efficient and large-scale animal cell culture, it is necessary to develop and produce animal bioreactors that can meet high-efficiency and large-scale production. The so-called animal bioreactor is a device for large-scale ani...

AI Technical Summary

Problems solved by technology

Its main disadvantage is: the product cannot be cultured at high density for adherent cells, and it is reported that it is at least 10 times lower than static perfusion culture

Secondly, different types of WAVE culture bags need to use matching shakers. Small bags can use large shakers, and large bags can’t use small shakers. It is necessary to purchase a series of expensive equipment

AMP torrent culture device has the advantages of high dissolved oxygen, low shear force and no cross-contamination of disposable torrent reaction bags, but it can only be used for static culture of large-scale adherent cells, not for suspension of non-adherent cells culture, because the circulation of the medium generated by the peristaltic pump repeatedly squeezes the cells cultured in suspension

The equipment is also more expensive and more complicated to operate

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