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Tetragenous detection card of clenbuterol, ractopamine, salbutamol and tubertaline and preparation method thereof

A technology of ractopamine and terbutaline, which can be applied to measurement devices, instruments, scientific instruments, etc., can solve the problem of not being able to detect beta stimulants at the same time, and achieve the effect of simple method and accurate results.

Inactive Publication Date: 2011-09-07
冯晓阳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to overcome the technical defect of being unable to simultaneously detect four beta-agonists of clenbuterol, ractopamine, salbutamol and terbutaline, the object of the present invention is to provide a method capable of simultaneously detecting clenbuterol, ractopamine, salbutamol and terbutaline. Quadruple detection kit for terbutaline

Method used

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  • Tetragenous detection card of clenbuterol, ractopamine, salbutamol and tubertaline and preparation method thereof
  • Tetragenous detection card of clenbuterol, ractopamine, salbutamol and tubertaline and preparation method thereof
  • Tetragenous detection card of clenbuterol, ractopamine, salbutamol and tubertaline and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0028] Preparation of quadruple detection card:

[0029] (1) Preparation of colloidal gold solution: take a clean Erlenmeyer flask, accurately weigh 100g of pure water on a balance, move to the heating mantle, stir and heat quickly to boiling, add 1ml of 1% chloroauric acid solution after boiling, stir well Quickly add 2ml of 1% trisodium citrate, keep the temperature at 90-100°C, continue to stir, when the color of the solution turns purple, continue to heat for 10min, take it out to cool at room temperature, and store at 4°C;

[0030] (2) Antibody pretreatment: High-concentration antibodies (≥2mg / ml) will aggregate to varying degrees during long-term cryopreservation. These aggregates will affect the stability of the label, so treatment is required before labeling. Dilute the clenbuterol or ractopamine or salbutamol or terbutaline monoclonal antibody to be labeled with 0.01mol / LPBS to 1mg / ml, pass through a 0.22um filter membrane; or the labeled clenbuterol or ractopamine or s...

Embodiment 2

[0038] Minimum detection limit test

[0039] (1) Clenbuterol minimum detection limit test

[0040] Accurately weigh 0.500g clenbuterol antigen, dissolve it in distilled water and dilute to 100mL, mark it as A 1 liquid, take A 1 Add 1mL of distilled water to the volumetric flask and make the volume to 1000mL to form A 2 liquid, take A 2 Liquid 1mL to volumetric flask, add distilled water to 1000mL to form A 3 liquid, then A 3 The concentration of the liquid is 5 / 1000000000g / mL (that is, 5ng / mL (5 nanograms / mL, 5ppb). Take A 3 Liquid 1mL in 4 test tubes, and labeled respectively. Inject 9 mL of distilled water into No. 1 test tube, inject 4 mL of distilled water into No. 2 test tube, inject 1 mL of distilled water into No. 3 test tube, and add no medicine to No. 4 test tube. Take another empty test tube, and inject 5mL of distilled water into it, and mark it as No. 5 test tube. The concentrations of clenbuterol antigen solution in test tubes 1 to 5 are: 0.5ppb, 1ppb, 2.5...

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Abstract

The invention relates to a tetragenous detection card of clenbuterol, ractopamine, salbutamol and tubertaline and a preparation method thereof. In the invention, a test bar is arranged in a detection card housing and comprises a sample pad, a colloidal gold film, a coating film and an absorbent cotton which are bonded onto a PVC (Polyvinyl Chloride) supporting back plate in sequence; the colloidal gold film is a glass fiber film containing colloidal gold markers of an anti-clenbuterol monoclonal antibody, an anti-ractopamine monoclonal antibody, an anti-salbutamol monoclonal antibody and an anti-tubertaline monoclonal antibody; the coating film is an acetate fiber film on which four detection strips and a quality control strip are arranged; and the detection strips comprises a detection strip containing clenbuterol antigen, a detection strip containing ractopamine antigen, a detection strip containing salbutamol antigen and a detection strip containing tubertaline antigen in sequence, and the quality control strip contains a goat-anti-mouse antibody. By using the tetragenous detection card and the preparation method thereof, provided by the invention, the clenbuterol, the ractopamine, the salbutamol and the tubertaline can be simultaneously detected; and the tetragenous detection card has the advantages of simple, convenient and fast method and accurate result.

Description

technical field [0001] The invention relates to a device for detecting beta receptor agonists, in particular to a clenbuterol, ractopamine, salbutamol and terbutaline quadruple detection card and a preparation method thereof. Background technique [0002] In today's society, the importance of food safety has been paid more and more attention. For example, the "clenbuterol" poisoning incident, the "melamine" incident in China in 2008, etc., have made food safety the focus of global attention. Pigs are the world's largest animal-sourced food, and the quality of pork has attracted the attention and attention of many countries. [0003] The quality of pork is usually determined by the quality of feed. In the past, clenbuterol (clenbuterol) was used as a feed additive, which was an effective method to increase the lean meat rate of pigs and reduce fat deposition. The effect of meat rate. In the mid-1980s, European and American countries discovered that "Clenbuterol" had seriou...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558G01N33/532G01N33/543
Inventor 冯晓阳
Owner 冯晓阳
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