Method for producing high-methionine and high-lysine feed by using composite bacteria fermented corn protein powder
A technology of high methionine lysine and corn gluten meal, which is applied in the direction of animal feed, animal feed, additional food elements, etc. The intake can not meet the growth needs and is not easy to be absorbed by animals, so as to achieve the effect of good absorption by animals, improve feed utilization, and facilitate storage.
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Embodiment 1
[0025] Step 1: Cultivate Corynebacterium, Yeast and Bacillus subtilis separately to prepare bacteria liquids of Corynebacterium, Yeast and Bacillus subtilis respectively. The number of live bacteria in the liquid reaches 200 million / ml, and the number of live bacteria in the liquid of Bacillus subtilis reaches 1 billion / ml;
[0026] In this step, corynebacteria can be cultivated using conventional medium, preferably beef extract medium, and the cultivation conditions of corynebacteria are: pH value 6.5, temperature 32°C; yeast can be cultivated using conventional medium, preferably wort medium , Culture conditions: temperature 30°C; Bacillus subtilis can be cultivated in conventional medium, preferably beef extract medium, culture conditions: pH value 6.5, temperature 32°C.
[0027] Step 2: The three kinds of strain liquids are added to the fermenter according to the weight ratio of 70% of Corynebacterium, 20% of yeast, and 10% of Bacillus subtilis to expand the cultivation. T...
Embodiment 2
[0034] Step 1: Cultivate Corynebacterium, Yeast and Bacillus subtilis separately to prepare bacteria liquids of Corynebacterium, Yeast and Bacillus subtilis respectively. The number of live bacteria in the liquid reaches 300 million / ml, and the number of live bacteria in the liquid of Bacillus subtilis reaches 1.2 billion / ml;
[0035] In this step, corynebacteria can be cultured using conventional medium, preferably beef extract medium, and the culture conditions of corynebacteria are: pH value 7, temperature 34°C; yeast can be cultured using conventional medium, preferably wort medium , Culture conditions: temperature 30°C; Bacillus subtilis can be cultured in conventional medium, preferably beef extract medium, culture conditions: pH value 6.5, temperature 37°C.
[0036] Step 2: The three kinds of strain liquids are added to the fermenter according to the weight ratio of 70% of Corynebacterium, 20% of yeast, and 10% of Bacillus subtilis to expand the cultivation. The composi...
Embodiment 3
[0043] Step 1: Cultivate Corynebacterium, Yeast and Bacillus subtilis separately to prepare bacteria liquids of Corynebacterium, Yeast and Bacillus subtilis respectively. The number of live bacteria in the liquid reaches 500 million / ml, and the number of live bacteria in the liquid of Bacillus subtilis reaches 1 billion / ml;
[0044] In this step, corynebacteria can be cultured using conventional medium, preferably beef extract medium, the culture conditions of corynebacteria: pH value 7.5, temperature 32°C; yeast can be cultured using conventional medium, preferably wort medium , Culture conditions: temperature 30°C; Bacillus subtilis can be cultivated in conventional medium, preferably beef extract medium, culture conditions: pH value 6.5, temperature 35°C.
[0045] Step 2: The three kinds of strain liquids are added to the fermenter according to the weight ratio of 70% of Corynebacterium, 20% of yeast, and 10% of Bacillus subtilis to expand the cultivation. The composition o...
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