Vaccine for treatment/prevention of scuticociliatosis in fish

A technology of scutellaria and mixed vaccines, which is applied in vaccines, veterinary vaccines, antibody medical components, etc., can solve the problem of invading host fish, etc., and achieve environmental sanitation improvement, productivity and quality improvement, and simple inactivation method Effect

Inactive Publication Date: 2011-09-14
NAT UNIV CORP EHIME UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, it has been confirmed that there is an insecticidal effect (non-patent document 3) by immersion in formalin etc., and an effect has also been confirmed by a treatment method using a medicated bath containing hydrogen peroxide (patent document 1), but, for the tongue In the case of the perch filthophilus, which is internally parasitic and invades the brain of the host fish, it cannot be said that a sufficient effect has been achieved

Method used

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  • Vaccine for treatment/prevention of scuticociliatosis in fish
  • Vaccine for treatment/prevention of scuticociliatosis in fish
  • Vaccine for treatment/prevention of scuticociliatosis in fish

Examples

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Embodiment

[0054] The present invention will be described more specifically through examples below, but this is not a limitation of the present invention.

reference example 1

[0056] Inactivation assay and Westren blotting to compare the antigenicity of F. glasophila isolated from different fish species and different geographic regions.

[0057] (1) Inactivation assay

[0058] Iyo I, Nakajima, Mie0301, and SK05Kyo isolates of Filophila glastris were selected, and rabbit serum was prepared. The test strains used were Iyo I strain, Nakajima strain, Mie0301 strain, JF05To strain, RF05To strain, and SK05Kyo strain. The inactivation assay is to use the above-mentioned antiserum diluted 20, 40, 80, 160, 320, 640, 1280 and 2560 times to sensitize each strain (about 100 worms) of the perch perch, and observe under the microscope Agglutination and inactivation, to find the inactivation titer.

[0059] The results are shown in Table 2. The anti-Iyo I strain serum (antibody) showed an agglutinating antibody titer of 80 times or more to Iyo I strain, JF05To strain, RF05To strain, and SK05Kyo strain, respectively. However, the Nakajima strain and the Mie0301...

Embodiment 1

[0071] Embodiment 1: the effectiveness test of 3 kinds of mixed vaccines

[0072] (1) Production of vaccines

[0073] (a) Test fish

[0074] The following fish were used: 200 fry of flounder were fed for 2 weeks using a feed for marine seedlings of flounder Otohime (manufactured by Nissin Marubeni Feed Co., Ltd.), and preliminarily reared to an average weight of 10.3 g.

[0075] (b) Culture of the worm

[0076] The worm was cultured for 5 days on fish cell line CHSE-214 cells that had been used in Eagle's MEM medium "NISSUI" (manufactured by Nissui Pharmaceutical Co., Ltd.) A medium obtained by mixing 10% fetal bovine serum (FBS) and adjusting the pH to 7.3 with 7.5% sodium bicarbonate water was cultured to a monolayer.

[0077] (c) Preparation of formalin inactivated vaccine

[0078] This worm (Iyo I strain, Nakajima strain and Mie0301 strain) 1.65×10 of above-mentioned cultivation 6 ~9.85×10 6 The cells were added with 35% formalin (manufactured by Nacalai Tesque) to a...

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Abstract

Disclosed is an effective prophylactic / therapeutic vaccine which can protect from scuticociliatosis, particularly can protect from the infection by Philasterides dicentrarchi that has a high mortality rate. The vaccine comprises (1) an inactivated product of a strain having the same serum type as that of P. dicentrarchi strain Iyo I, (2) an inactivated product of a strain having the same serum type as that of P. dicentrarchi strain Nakajima, and (3) an inactivated product of a strain having the same serum type as that of P. dicentrarchi strain Mie0301 as active ingredients.

Description

technical field [0001] The present invention relates to a vaccine effective for the prevention or treatment of scuticociliatosis. Background technique [0002] Scutellaria is a fish parasitic disease that was first confirmed in Japanese flounder (ヒラメ) in 1990. The production value of farmed flounder in Japan in 2004 was about 9.3 billion yen. About 20% of them are from Ehime Prefecture, and among the number of flounder fish disease diagnoses reported by the fish disease guidance center in this prefecture, the diagnosis rate of scutellum disease is as high as about 15-20% in a year. It is estimated that, The mortality rate of the disease is 30-70%. For the flounder aquaculture industry, the disease has become an important fish disease, but the current situation is that the treatment and prevention methods for the disease have not been established, and some practitioners even have to give up the flounder aquaculture industry due to the disease. Since then, not only in Japan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61P33/00
CPCA61K39/002A61K2039/552A61P33/00C12N1/20A61K2039/522
Inventor 北村真一宋准荣
Owner NAT UNIV CORP EHIME UNIV
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