Method for preparing osmanthus concrete and osmanthus total flavonoid through enzyme hydrolysis technology

Abstract

The invention discloses a method for preparing osmanthus concrete and osmanthus total flavonoid through an enzyme hydrolysis technology. The method comprises the following steps of: carrying out vacuum drying; carrying out grinding treatment; carrying out solid enzyme hydrolysis by using a beta-D-glycuronide solution prepared from condensed water collected in a cooling way through the vacuum drying; extracting the osmanthus concrete through petroleum ethers; extracting a crude product of the osmanthus total flavonoid through the assistance of ultrasonic waves; and refining the osmanthus total flavonoids. According to the method, the problem of preservation of fresh osmanthus is effectively solved; the loss of water-soluble aroma components and flavonoid functional components in a water-phase solution is reduced; and the extraction yield and the product quality of the osmanthus concrete are effectively enhanced. The osmanthus concrete prepared by adopting the method has product yield more than and equal to 7 percent (in terms of dry osmanthus) and absolute oil content more than and equal to 90 percent; and a crude extract of the osmanthus total flavonoid is enhanced in product yield by more than 10 percent and increased in total flavonoid content to more than 80 percent, and therefore, the value of a product is effectively enhanced and the application range of the product is effectively enlarged.

Description

technical field [0001] The invention relates to a method for preparing sweet-scented osmanthus extract and total osmanthus flavonoids by enzymatic hydrolysis technology. The main products are sweet-scented osmanthus extract and total osmanthus flavonoids, belonging to the technical field of biotechnology extraction of natural plant spices and other active ingredients. Background technique [0002] Osmanthus fragrans is one of my country's ten traditional famous flowers, also known as rock osmanthus, jiulixiang, and sweet-scented osmanthus, belonging to the genus Oleaceae Oleaceae, and has been cultivated in my country for more than 2,500 years. Osmanthus fragrans is very popular among the Chinese people because of its strong fragrance and long lasting taste. For a long time, in addition to being used to make various foods, osmanthus fragrans has been used as a raw material for natural plant spices to extract osmanthus fragrans extract and is widely used in food, daily cosmet...

AI Technical Summary

Problems solved by technology

But this method still has following deficiencies: 1: this method adopts vacuum microwave technology to carry out osmanthus drying process, and the osmanthus fragrance substance volatilized in this process is not reclaimed, causes the reduction of sweet-scented osmanthus fragrance product yield and problems such as impure fragrance

During this wet enzymatic hydrolysis process, more water-soluble aroma components and flavonoid functional active ingredients will be lost in the aqueous phase solution, which will also lead to a decrease in the yield of flavor products and flavonoid extracts and impure aromas, etc. question

3: This method uses the conventional ethanol extraction method to extract the flavonoids in the flower residue, the extraction time is longer, the extraction temperature is higher, and the product activity and product yield are lower

4: This method does not involve the refining process of the crude extract of total flavonoids from osmanthus fragrans, and the product value and application range of the crude extract of total flavonoids are greatly limited