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Composite microbial preparation for purifying water and preparation method thereof

A compound preparation and water purification technology, which is applied in the field of microbial fermentation, can solve the problems of increasing the culture cycle and increasing costs, and achieve the effect of shortening the culture cycle and reducing COD

Active Publication Date: 2012-12-12
SHANGHAI SHUIPINGHENG ENVIRONMENT TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the existing microbial preparations, in order to make the microorganisms exert their maximum effect, improvements will be made on the microbial preparations and the equipment used, such as adding activating enzymes to activate microorganisms, adding water purification plants to the preparations, and using equipment to spray and increase the microbial preparations. The area in contact with the water body, etc., these methods sometimes cannot make the bacteria fully grow, and increase the culture period, and also greatly increase the cost during preparation and use.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0036] 1. Preparation of microbial compound preparations:

[0037] (1) The seed liquid of each strain in the microbial compound preparation is prepared separately:

[0038] a. Preparation of Bacillus licheniformis (Bacillus licheniformis) seed culture solution: Take 10 μl of Bacillus licheniformis (Bacillus licheniformis) glycerol seed preservation bacteria into 1ml liquid LB medium in the evening of the first day, put it into a constant temperature shaker at 37°C, Cultivate overnight at 200r / min, and in the morning of the second day, take 500μl of the above bacterial solution and transfer it to a 50ml liquid medium seed bottle, and culture at 37°C with shaking at 200r / min for 12 hours, and the number of Bacillus licheniformis ≥ 1.0×10 9 cfu / ml seed culture solution. The liquid LB medium is: add 30 grams of brown sugar, 10 grams of tryptone, 5 grams of yeast, 10 grams of sodium chloride and 2 grams of sodium dihydrogen phosphate in 1L of distilled water, adjust the pH to 7.5-...

Embodiment 1

[0055] Purify river water quality

[0056] Applied to 10,000 cubic meters of inferior V water body, the average water quality indicators of 10 sampling points before application are: COD is 178.25mg / L, total nitrogen is 14.89mg / L, ammonia nitrogen is 10.31mg / L, and total phosphorus is 6.795 mg / L.

[0057] How to use: Diluted at a concentration of 5ppm and poured into the whole water, use it for 3 consecutive days, once a day. After that, once a week, the whole water body is splashed 6 times.

[0058] Test results: One month later, water samples were taken at the same sampling point, and the average water quality indicators measured were: COD was 34.95mg / L, a decrease of 80.4%; total nitrogen was 2.21mg / L, a decrease of 85.2%; ammonia nitrogen was 1.37 mg / L, decreased by 86.7%; total phosphorus was 1.352mg / L, decreased by 80.1%. The measured water body indicators are basically close to the standard limit of Class V water.

Embodiment 2

[0060] Purify lake water quality

[0061] Applied to 50,000 cubic meters of inferior V water body, the average water quality indicators of 20 sampling points before application are: COD is 86.70mg / L, total nitrogen is 5.31mg / L, ammonia nitrogen is 3.09mg / L, and total phosphorus is 0.773 mg / L.

[0062] How to use: Diluted at a concentration of 5ppm and poured into the whole water, use it for 3 consecutive days, once a day. After that, once a week, the whole water body is splashed 6 times.

[0063] Test results: One month later, water samples were taken at the same sampling point, and the average water quality indicators measured were: COD was 15.70 mg / L, a decrease of 81.9%; total nitrogen was 1.24 mg / L, a decrease of 76.7%; ammonia nitrogen was 0.89 mg / L, decreased by 71.2%; total phosphorus was 0.170mg / L, decreased by 78%, and the measured water body indicators were all lower than the standard limit of Class IV water.

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Abstract

The invention discloses a composite microbial preparation for purifying water and a preparation method thereof. The preparation method is characterized by comprising the following steps of: respectively carrying out single-strain culture on bacillus licheniformis, bacillus subtilis, bacillus circulans, xanthan-degrading bacteria and fusiform bacillus to obtain seed culture solutions; inoculating into a same culture medium; then carrying out seed tank fermentation and fermentation tank fermentation culture to obtain the product preparation, wherein the microbial contents of five strains contained in the product preparation are all more than and equal to 1.0*10<9> cfu / ml. According to the preparation method, the strains sufficiently grow, and therefore, the effectiveness of the strains is not reduced and a culture period is also greatly shortened; after a polluted water body is added, the strains respectively perform own functions and cooperatively play roles, and therefore the content of COD (Chemical Oxygen Demand), total nitrogen, total phosphorus and ammonia nitrogen which are contained in water is effectively reduced.

Description

technical field [0001] The invention relates to a microbial composite preparation for purifying water quality of river courses, lakes and landscape water bodies, belongs to the field of microbial fermentation, and particularly relates to a microbial suture preparation for purifying water quality and a preparation method thereof. Background technique [0002] With the continuous improvement of living standards, people put forward higher requirements for living conditions and living environment. A large number of artificially designed waterscapes have been applied in the construction of new parks and living quarters, adding a beautiful scenery to people. Since these landscapes are artificially created, in order to make them have the ability of self-purification in a short period of time, it is necessary to quickly establish a dynamic and balanced ecosystem to play a role, in which microorganisms play a pivotal role. [0003] At the same time, with the intensification of human...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/20C02F3/34C12R1/10C12R1/125C12R1/09C12R1/01
Inventor 谢平
Owner SHANGHAI SHUIPINGHENG ENVIRONMENT TECH DEV CO LTD
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