Method for extracting forsythin and forsythoside from forsythia leaves

A crude technology of forsythiaside and forsythiaside, which is applied in the field of natural product separation, can solve the problem that forsythin and forsythiaside cannot be simultaneously obtained by extracting and separating forsythin and forsythiaside at the same time. Effective ingredients, hydrolysis and destruction of Forsythia leaves, etc., to achieve the effect of simple method, low production cost and good product quality

Inactive Publication Date: 2011-10-19
河南省科高植物天然产物开发工程技术有限公司
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  • Application Information

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Problems solved by technology

[0005] The deficiencies in the existing methods: (1) the water extraction in the existing methods mostly adopts the mixing and soaking of forsythia leaves and water and then heating and extracting, and this This traditional extraction method using soaking and heating will lead to the hydrolysis and destruction of some active ingredients in Forsythia leaves, such as forsy

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  • Method for extracting forsythin and forsythoside from forsythia leaves
  • Method for extracting forsythin and forsythoside from forsythia leaves

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Embodiment 1

[0032] A method for extracting forsythin and forsythiaside from forsythia leaves, the method comprising the steps of:

[0033] ①Extraction: Add 5kg of forsythia leaves into water at 80°C (50kg of water) for ultrasonic extraction for 1 hour, ultrasonic power 20KHz, and extract twice;

[0034] ②Separation: Combine the extracts obtained twice, filter and separate to discard the residue, concentrate the filtrate under reduced pressure (the volume ratio of the filtrate before and after concentration is 8:1), let it stand at room temperature for 15 hours, and then filter and separate the precipitate and the water layer under normal pressure. The precipitate is The crude extract of forsythin, the aqueous layer is the crude extract of forsythin;

[0035] ③ Refined Forsythin: Dry the crude extract of Forsythin, then mix 200g of the crude extract of Forsythin with ethyl acetate at a weight ratio of 1:6, then reflux extraction at 55°C for 2 times, each time for 40min , combined the ethy...

Embodiment 2

[0038] A method for extracting forsythin and forsythiaside from forsythia leaves, the method comprising the steps of:

[0039] ①Extraction: Add 10kg of forsythia leaves to 55°C water (160kg of water) for extraction, 1.5h each time, 3 times;

[0040] ②Separation: Combine the extracts obtained three times, filter and separate at normal pressure to discard the residue, concentrate the filtrate under reduced pressure (the volume ratio of the filtrate before and after concentration is 12:1), let it stand at room temperature for 48 hours, and then centrifuge at 3000r / min high-speed centrifuge to separate the precipitate and In the water layer, the precipitate is the crude extract of forsythin, and the water layer is the crude extract of forsythin;

[0041] ③ Refined forsythin: dry the crude extract of forsythin, then mix the dried 380g crude extract of forsythin with ethyl acetate at a weight ratio of 1:4, then reflux extraction at 60°C for 2 times, each time for 30 minutes , combi...

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Abstract

The invention relates to a method for extracting forsythin and forsythoside from forsythia leaves. The method comprises the steps of: taking forsythia leaves as raw materials, and firstly carrying out extraction, concentration and separation with hot water, thus obtaining rough forsythin extract and rough forsythoside extract liquid; extracting the forsythin extract with ethyl acetate, and carrying out recrystallization with alcohol, thus obtaining a forsythin product with purity of 99 percent; and extracting, concentrating and drying the rough forsythoside extract liquid with water-saturatedn-butyl alcohol, thus obtaining a rough forsythoside product with purity more than 40 percent, or more than 90 percent through further purification. The method is simple for process, high in raw material utilization ratio, high in purity of the product and suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of natural product separation, and in particular relates to a method for extracting and separating forsythin and forsythiaside from forsythia leaves. Background technique [0002] Forsythia is a deciduous shrub belonging to Forsythiaceae of Oleaceae. It is one of the most common medicinal materials in traditional Chinese medicine. Modern pharmacological research shows that forsythia has various pharmacological effects such as antibacterial, antiviral, antipyretic and anti-inflammatory, anti-oxidation, and liver protection. Our country is rich in forsythia resources, which are distributed in the north and south, with the largest output in Henan, Shanxi and Shaanxi. The chemical composition of forsythia mainly has three categories: lignans (such as forsythin, forsythiaside, etc.), triterpene acids (such as oleanolic acid, ursolic acid, etc.) and volatile oils. According to research, the components of the lea...

Claims

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Application Information

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IPC IPC(8): C07H15/26C07H15/18C07H1/08
Inventor 李晓魏悦郭唯张培林范毅董建军赵天增张海燕李倩于立芹陈玲
Owner 河南省科高植物天然产物开发工程技术有限公司
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