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Method for producing glycyrrhizic acid through enzymolysis

A production method and glycyrrhizic acid technology are applied in the field of compound enzymatic extraction of glycyrrhizic acid to achieve the effects of environmental friendliness, simple operation and high purity

Inactive Publication Date: 2011-10-19
BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The new method for preparing glycyrrhizic acid of the present invention has not yet been reported at home and abroad

Method used

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  • Method for producing glycyrrhizic acid through enzymolysis
  • Method for producing glycyrrhizic acid through enzymolysis
  • Method for producing glycyrrhizic acid through enzymolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Take licorice ( Glycyrrhiza uralensis Fisch.) medicinal material 500g, pulverized, passed through 10-20 mesh sieve.

[0036] (2) Add distilled water twice the weight of the licorice medicinal material for soaking, and add 1.6 g of cellulase, 0.8 g of pectinase, 10 mg of xylanase, and 10 mg of β-glucanase per 500 g of medicinal material.

[0037] (3) Adjust the pH to 5.0-6.0 with HCl, enzymatically hydrolyze at 50°C for 2 hours, then boil for 2 minutes to inactivate the enzyme, add ethanol to make the final concentration of ethanol reach 10% (the ethanol concentration shown here and below are all volume percentages ).

[0038] (4) The extraction conditions of 10% ethanol are: heat to 80°C for 2 hours, repeat heating 3 times, combine the extracts 3 times, filter the extracts, centrifuge, and take the supernatant.

[0039] (5) Concentrate the supernatant under reduced pressure to obtain the extract, add a small amount of water to dissolve the extract, and adjust the...

Embodiment 2

[0043] (1) Glycyrrhiza officinalis ( G. inflate .Bat.) Herbs 500g, pulverized, passed through 10-20 mesh sieve.

[0044] (2) Add distilled water 3 times the weight of the licorice medicinal material for soaking, and add 2 g of cellulase, 0.8 g of pectinase, 10 mg of xylanase, and 10 mg of β-glucanase per 500 g of medicinal material.

[0045] (3) Adjust the pH to 5.0-6.0 with HCl, enzymatically hydrolyze at 50°C for 3 hours, then boil for 2 minutes to inactivate the enzyme, add ethanol to make the final concentration of ethanol reach 10%.

[0046] (4) The extraction conditions of 10% ethanol are as follows: heat to 80°C for 2 hours, repeat heating 3 times, and combine the extracts 3 times. The extract was filtered, centrifuged, and the supernatant was taken.

[0047] (5) Concentrate the supernatant under reduced pressure to obtain the extract, add a small amount of water to dissolve the extract, and adjust the pH to 6.2-6.5 with dilute ammonia water.

[0048] (6) Slowly add...

Embodiment 3

[0051] (1) Glycyrrhiza glabra ( G. glabra. L.) 500g of medicinal materials, pulverized and passed through a 10-20 mesh sieve.

[0052] (2) Add distilled water twice the weight of the licorice medicinal material for soaking, and add 1.6 g of cellulase, 1 g of pectinase, 10 mg of xylanase, and 10 mg of β-glucanase per 500 g of medicinal material.

[0053] (3) Adjust the pH to 5.0-6.0 with HCl, enzymatically hydrolyze at 50°C for 4 hours, then boil for 2 minutes to inactivate the enzyme, add ethanol to make the final concentration of ethanol reach 10%.

[0054] (4) The extraction conditions of 10% ethanol are as follows: heat to 80°C for 2 hours, repeat heating 3 times, and combine the extracts 3 times. The extract was filtered, centrifuged, and the supernatant was taken.

[0055] (5) Concentrate the supernatant under reduced pressure to obtain the extract, add a small amount of water to dissolve the extract, and adjust the pH to 6.2-6.5 with dilute ammonia water.

[0056] (6)...

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Abstract

The invention provides a method for producing glycyrrhizic acid through enzymolysis. The method comprises the steps of carrying out enzymolysis on glycyrrhiza uralensis by utilizing complex enzyme, extracting glycyrrhizic acid with 10 percent volume of alcohol, separating and purification with macroporous adsorption resin, and eluting with 10 percent volume alcohol as eluent, thus obtaining glycyrrhizic acid, wherein the complex enzyme is of a mixture of pectinase, cellulose, xylanase and beta-glucanase. The extraction method has the advantages of low cost, simplicity and convenience in operation, environment friendliness, high yield and purity, and suitability for industrial production.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy or raw material processing, and in particular relates to a production method for extracting glycyrrhizic acid by a compound enzyme method. Background technique [0002] Glycyrrhizae is a kind of Chinese herbal medicine with a wide range of uses. Clinical and pharmacological studies have shown that glycyrrhizic acid has obvious anti-inflammatory, anti-virus, immune regulation, blood lipid-lowering and anti-atherosclerotic effects. It has a wide range of uses in the cosmetics and cosmetics industry, so the research on the extraction and separation of glycyrrhizic acid has attracted more and more attention from the world. [0003] The water extraction method and solvent method used to extract glycyrrhizic acid are relatively mature technology, and the extraction and refining production methods are still relatively backward. There are obvious deficiencies in these conventional met...

Claims

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Application Information

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IPC IPC(8): C07J63/00C07H15/256C07H1/08
Inventor 葛喜珍姜楠林强田平芳
Owner BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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