Inorganic phosphate solubilizing bacteria capable of promoting accumulation of dry matter in eucalyptus
A phosphate solubilizing bacteria and inorganic technology, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve problems such as difficult to distinguish clearly, single phosphorus solubilizing ability, etc., achieve good effect, strong promotion effect, and improve utilization rate Effect
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Embodiment 1
[0019] Embodiment 1: the acquisition of inorganic phosphate-solubilizing bacteria strain P19
[0020] one, The isolation and screening process of inorganic phosphate-solubilizing bacteria strain P19 is as follows:
[0021] (1) Collect eucalyptus rhizosphere soil in the field. The soil was taken from the Yongan State-owned Forest Farm in Fujian Province, which is located in the suburbs of Yongan City, with the latitude and longitude of E117°23'18", N25°56'27".
[0022] (2) Isolation of inorganic phosphate-solubilizing bacteria
[0023] ①Preparation of rhizosphere soil suspension: In a sterile room, accurately weigh 5 g of fresh soil samples, put them into a 250 ml Erlenmeyer flask filled with 95 ml of sterile water, shake on a shaker for 20 min, and let the microorganisms Disperse the cells, let stand for 20-30 s, and serve 10 -1 Diluent; according to the method of 10-fold step-by-step dilution, serially dilute to make 10 -4 、10 -5 、10 -6 、10 -7 、10 -8 、10 -9 A serie...
Embodiment 2
[0102] Embodiment 2: the influence of bacterial strain P19 on the growth of eucalyptus seedlings
[0103] The soil for testing is black peat soil (from Jianxin flower market in Fuzhou), pH value is 7.23, available phosphorus is 5 mg·kg -1 . The organic fertilizer was purchased from Jianxin Flower Market in Fuzhou, and the eucalyptus seedlings tested were from Fujian Forestry Science Research Institute, the clone of Eucalyptus giantus No. 5. Set blank, apply P control (KH 2 PO 4 ), organic fertilizer (4% nitrogen, 3% phosphorus, 4% potassium, 30% organic matter, 25% water, 10% humic acid) and single bacteria P19. The inoculum volume of strain P19 inoculated in Eucalyptus was 50 ml culture solution per pot / (1×10 6 CFU·ml -1 ), repeated three times in three days, treated with distilled water as a blank control. Apply P fertilizer as KH per pot 2 PO 4 5g, 5 kg of soil, repeated 3 times. The organic fertilizer was 20 g per pot, filled with 5 kg of soil, and repeated 3 tim...
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