Inorganic phosphate solubilizing bacteria capable of promoting accumulation of dry matter in eucalyptus

A phosphate solubilizing bacteria and inorganic technology, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve problems such as difficult to distinguish clearly, single phosphorus solubilizing ability, etc., achieve good effect, strong promotion effect, and improve utilization rate Effect

Inactive Publication Date: 2011-11-02
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

There are great differences in the phosphorus-solubilizing effects of the three types of phosphorus-solubilizing microorganisms. The number of fungi is less than that of bacteria, but the phosphorus-solubilizing ability of some fungi is significantly greater than that of bacteria
However, many inorganic phosphorus-decomposing microorganisms also have the ability to decompose organic phosphorus, so it is difficult to distinguish them clearly in practice. Some bacteria only have a single phosphorus-solubilizing ability, and some bacteria have two phosphorus-solving abilities , the latter is an ideal screening object

Method used

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  • Inorganic phosphate solubilizing bacteria capable of promoting accumulation of dry matter in eucalyptus
  • Inorganic phosphate solubilizing bacteria capable of promoting accumulation of dry matter in eucalyptus

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Embodiment 1

[0019] Embodiment 1: the acquisition of inorganic phosphate-solubilizing bacteria strain P19

[0020] one, The isolation and screening process of inorganic phosphate-solubilizing bacteria strain P19 is as follows:

[0021] (1) Collect eucalyptus rhizosphere soil in the field. The soil was taken from the Yongan State-owned Forest Farm in Fujian Province, which is located in the suburbs of Yongan City, with the latitude and longitude of E117°23'18", N25°56'27".

[0022] (2) Isolation of inorganic phosphate-solubilizing bacteria

[0023] ①Preparation of rhizosphere soil suspension: In a sterile room, accurately weigh 5 g of fresh soil samples, put them into a 250 ml Erlenmeyer flask filled with 95 ml of sterile water, shake on a shaker for 20 min, and let the microorganisms Disperse the cells, let stand for 20-30 s, and serve 10 -1 Diluent; according to the method of 10-fold step-by-step dilution, serially dilute to make 10 -4 、10 -5 、10 -6 、10 -7 、10 -8 、10 -9 A serie...

Embodiment 2

[0102] Embodiment 2: the influence of bacterial strain P19 on the growth of eucalyptus seedlings

[0103] The soil for testing is black peat soil (from Jianxin flower market in Fuzhou), pH value is 7.23, available phosphorus is 5 mg·kg -1 . The organic fertilizer was purchased from Jianxin Flower Market in Fuzhou, and the eucalyptus seedlings tested were from Fujian Forestry Science Research Institute, the clone of Eucalyptus giantus No. 5. Set blank, apply P control (KH 2 PO 4 ), organic fertilizer (4% nitrogen, 3% phosphorus, 4% potassium, 30% organic matter, 25% water, 10% humic acid) and single bacteria P19. The inoculum volume of strain P19 inoculated in Eucalyptus was 50 ml culture solution per pot / (1×10 6 CFU·ml -1 ), repeated three times in three days, treated with distilled water as a blank control. Apply P fertilizer as KH per pot 2 PO 4 5g, 5 kg of soil, repeated 3 times. The organic fertilizer was 20 g per pot, filled with 5 kg of soil, and repeated 3 tim...

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Abstract

The invention provides inorganic phosphate solubilizing bacteria, which is a Lysinibacillussphaericus strain P19, with a collection number of CGMCC No.4769. When the inorganic phosphate solubilizing bacteria are inoculated onto eucalyptus, the accumulation of dry matter in eucalyptus can be promoted. The strain P19 has a high eucalyptus growth promoting effect, has high phosphate solubilizing capacity, can improve the soil phosphorus utilization rate of eucalyptus plants considerably, improve the accumulation of the dry matter in the eucalyptus to a great extent, and can achieve a better effect than applying phosphate fertilizer and organic fertilizer.

Description

technical field [0001] The invention relates to a Fusobacterium strain and application thereof, and more specifically relates to an inorganic phosphorus-solubilizing bacterium capable of promoting the accumulation of dry matter of eucalyptus. Background technique [0002] Together with pine and poplar, eucalyptus is known as the world's three fast-growing tree species. Because of its wide adaptability, high economic value, and both ecological and social benefits, it is favored by many countries. At present, eucalyptus has become a strategic tree species for the development of fast-growing and high-yield forests in southern my country. Due to the high temperature and humidity in the south, the organic matter decomposes quickly and is easy to be leached and lost, and the elements such as iron and aluminum are relatively accumulated, and the soil is acidic, which reduces the activity of available phosphorus. On the other hand, since eucalyptus is affected by the problem of sev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/01
Inventor 吴承祯洪伟洪滔谢安强俞新玲
Owner FUJIAN AGRI & FORESTRY UNIV
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