Extraction method of Chinese pine pollen small peptides

An extraction method and technology for pollen, which are applied in the field of extraction of pine pollen small peptides, can solve the problems of insufficient disclosure and insufficient description of the dissolution method of pine pollen, and achieve the effects of easy separation, suitability for consumption, and high water solubility.

Inactive Publication Date: 2011-11-02
承德畅达生物科技有限公司
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Problems solved by technology

[0004] Patent document CN101100691 discloses a method for extracting small peptides from Pinus tabulaeformis pollen with compound enzymes. Because the examiner believes that the application does not fully explain the dissolution method of Pinus t

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0024] The method for extracting small peptides from Pinus tabulaeformis pollen by enzymes in the present invention utilizes the catalytic reaction of compound enzymes to convert the protein in Pinus tabulaeformis pollen into active small molecular peptides that are easily absorbed by the human body. The extraction method comprises the following steps:

[0025] (1). Add 200 parts of Chinese pine pollen and 2000 parts of drinking water into an open container, grind and break the wall with a colloid mill, and when the broken wall reaches 95% under a microscope, separate it with a plate and frame filter press , to obtain broken wall pine pollen and filtrate;

[0026] (2). Take 150 parts of Pinus tabulaeformis pollen after breaking the wall, extract the soluble non-protein components of Pinus tabulaeformis pollen with carbon dioxide supercritical extraction; put the pollen of Pinus tabulaeformis after extraction in a container with a stirrer, at 45 ° C Under temperature conditions...

Embodiment 2

[0031] The pH value in the process step (2) in Example 1 is adjusted to 9, and the kind of enzyme in the process step (3) is changed from neutral protease to papain, and the reaction ends, and the system pH=5.8.

[0032] Other operating methods are with embodiment 1.

Embodiment 3

[0034] (1). Add 200 parts of Chinese pine pollen and 1000 parts of drinking water into an open container, grind and break the wall with a colloid mill, and when the broken wall reaches 95% under a microscope, separate it with a plate and frame filter press , to obtain broken wall pine pollen and filtrate;

[0035] (2). Take 100 parts of broken Chinese pine pollen, use 6# solvent oil (conforming to GB 16629-2008 standard) to extract the soluble non-protein components of Chinese pine pollen; put the extracted Chinese pine pollen into a mixer In a container, at a temperature of 50° C., adjust the solution to pH=10 with 1000 parts of filtrate, soak and stir for 3 hours.

[0036] (3). Add 0.2 part of alkaline protease, 0.1 part of neutral protease, and 0.2 part of papain to the above solution and stir fully for 3 hours. After the reaction is completed, pH=5.5;

[0037] (4). Inactivate the enzyme at 90°C for 30 minutes, cool to 40°C and centrifuge, remove the slag, and make a small...

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Abstract

The invention relates to an extraction method of Chinese pine pollen small peptides. The extraction method comprises the following steps of (1) mixing Chinese pine pollen and water, carrying out a wall breaking process on the Chinese pine pollen in a wet way through a colloid mill, and filtering, (2) extracting a soluble component from the Chinese pine pollen treated by the wall breaking process through adopting a solvent oil, (3) putting the Chinese pine pollen treated by the step 3 into a container with a stirrer, adding filtrate obtained from the step 1 into the Chinese pine pollen, adjusting a pH of the mixture to a pH of 8 to 9, and stirring the mixture for 2 to 4 hours at a temperature of 20 to 70 DEG C to obtain Chinese pine pollen mixed solution, and (4) carrying out processes of enzymatic hydrolysis and enzyme destruction, carrying out a centrifugal separation process, and removing residues to obtain nutrient solution rich in small peptides. The extraction method has a short production period and a high product yield. Nutrient components of extract produced by the extraction method are micro-molecule peptides which can be absorbed by human bodies easily. Active micro-molecule peptide nutrient solution prepared by the extraction method has a high water-solubility and can be used directly.

Description

technical field [0001] The invention belongs to the fields of molecular bioengineering and biochemical engineering, in particular to a method for extracting small peptides from Chinese pine pollen. Background technique [0002] Pinus tabulaeformis pollen contains all the nutrients needed by the human body, and with a reasonable mix, it can fully supplement and balance the nutrients needed by the human body, known as "green gold". Studies in recent years have shown that most of the final products of protein hydrolysis under the action of digestive enzymes in the digestive tract of animals are small peptides composed of 2 or 3 amino acid residues. The transfer between peptides has the characteristics of no competition and inhibition. The bio-enzyme engineering processing method hydrolyzes peptides and amino acids under mild reaction conditions to produce substances such as peptides and amino acids. It has high nutritional value and functional characteristics, and has controlla...

Claims

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Application Information

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IPC IPC(8): C12P21/06
CPCY02P20/54
Inventor 郭丽梅王贵玉薛润鹏尹树花
Owner 承德畅达生物科技有限公司
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