Peony bark nematicidal extract and preparation method thereof

A technology of Moutan Cortex and nematodes, which is applied in the field of Moutan Cortex nematicidal extract and its preparation, and can solve the problems of plant root-knot nematode control and other problems that have not been seen in Moutan Cortex ethyl acetate extract

Inactive Publication Date: 2011-11-23
BEIJING UNIV OF AGRI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

There is no relevant report on the application in agricultural production, and there is no relevant report on the use of ethyl acetate extract of Moutan Cortex for the prevention and treatment of plant root-knot nematode disease

Method used

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  • Peony bark nematicidal extract and preparation method thereof
  • Peony bark nematicidal extract and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, the acquisition of Cortex Moutan ethyl acetate extract

[0027] High-speed pulverizer, type 6202, manufactured by Beijing Huanya Tianyuan Machinery Technology Co., Ltd.; desktop three-frequency digital control ultrasonic cleaner, type KQ-500VDV, Kunshan Ultrasonic Instrument Co., Ltd.; rotary evaporator, type RE-2000, Shanghai Yarong Biochemical Co., Ltd. Instrument factory; stereo microscope, Motic SMZ-140, Motic Industrial Group Co., Ltd.; micro-adjustable pipette, GILSON, France; 96-well disposable cell culture plate, type, Canada; electric constant temperature incubator, SKP-02.420 type, Huangshi Hengfeng Medical Equipment Co., Ltd.; ethyl acetate (analytical pure), Beijing Chemical Plant;

[0028] The peony cortex Paeonia suffruticosa used in the test was purchased from Beijing Tongrentang Pharmacy, belonging to Ranunculaceae, and the root bark was used.

[0029] For the test root-knot nematode (Meloidogyne spp.), the diseased root of tomato root-kn...

Embodiment 2

[0044] Embodiment 2, poisonous activity assay method

[0045] The contact method is used for determination, and the specific steps are: take a 96-well cell culture plate, add 150 μL of the plant extract solution obtained by the method 1 of step 2 of Example 1 with a concentration of 20 mg / mL into each well, and then add an equal volume of Mix the root-knot nematode second-instar larvae suspension obtained in step 1, let it stand at 25°C, observe under a stereomicroscope after 12h, 24h and 48h respectively, and check whether the nematodes are dead or alive (needle contact method), stiff or curled. The worms were regarded as dead, and the corrected death rate of nematodes was calculated according to the formula. With sterile water as the control, each treatment was repeated 3 times, and the results were average ± standard error.

[0046]

[0047]

[0048]Data processing and analysis: The experimental data was analyzed and sorted by DPS2000, and the results were analyzed f...

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Abstract

The invention discloses a peony bark nematicidal extract and a preparation method thereof. The invention provides the preparation method of the peony bark ethyl acetate extract, which comprises the following steps of: (1) uniformly mixing the peony barks and ethyl acetate to obtain a mixture; and (2) ultrasonically treating the obtained mixture and filtering and concentrating the mixture to obtain the peony bark ethyl acetate extract. It is proved by an experiment provided by the invention that the peony barks are extracted by using the ethyl acetate and the poisoning effect of an extracting solution on root-knot nematode is tested; as a result, the extract shows strong poisoning activity to the root-knot nematode; and it is shown that the extract provided by the invention lays basis for the development and application of root-knot nematode biocontrol preparations in the future. According to the invention, nematicidal active substances are extracted by using natural and harmless plantmaterials so that the pollution caused by chemical pesticides can be reduced; and the simple extracting method is easy to promote and apply in controlling plant root-knot nematodes.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a nematicidal extract of Cortex Moutan and a preparation method thereof. Background technique [0002] Root-knot nematodes (Meloidogyne spp.) are obligate parasites on plant roots and are an important pathogen affecting agricultural production. [0003] Since the 1980s, the research on the use of plant extracts to control nematodes has gradually attracted people's attention. Up to now, there are about 102 families, 226 genera, and 316 species of nematode-killing plants, among which Asteraceae, Meliaceae, Bean family, Brassicaceae, Solanaceae, Poaceae, Euphorbiaceae, Poppyaceae, Apocynaceae, Mimosaceae, Liliaceae, etc., but the plant resources in nature are extremely rich, and many plants with nematicidal effects have not been discovered , the book "Poisonous Plants in China" has listed more than 1300 kinds of poisonous plants, many of which have insecticidal activity. Many of these...

Claims

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Application Information

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IPC IPC(8): A01N65/32A01P5/00
Inventor 魏艳敏宋庆丰马喆蔡建波尚巧霞张夏兰胡盼耿文龙
Owner BEIJING UNIV OF AGRI
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