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Method for leaching heavy metals in protein foods by using diluted acid and application thereof

A protein and heavy metal technology, applied in the fields of application, food preparation, food science, etc., can solve time-consuming and labor-intensive problems, achieve the effects of reducing pollution, speeding up extraction time, and realizing rapid on-site detection technology

Active Publication Date: 2011-11-23
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these heavy metal pretreatment techniques can accurately extract heavy metals in samples, they are relatively time-consuming and laborious. The obtained digestion solution is strongly acidic, and the enzyme reaction in ELISA needs to be carried out in a mild environment. Therefore, traditional sample pretreatment methods The obtained digestion solution cannot be directly applied to the detection of heavy metals by enzyme-linked immunoassay

Method used

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  • Method for leaching heavy metals in protein foods by using diluted acid and application thereof
  • Method for leaching heavy metals in protein foods by using diluted acid and application thereof
  • Method for leaching heavy metals in protein foods by using diluted acid and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Preparation of contaminated samples:

[0026] ① Omi Oyster samples:

[0027] A. Select 100 Omi oysters with a body size and weight difference of about ±10%, remove the attachments and wash them clean.

[0028] B. Add a solution containing heavy metals or heavy metal salts in seawater, the final concentrations of copper, lead and cadmium are 100 μg / L, 200 μg / L, and 25 μg / L respectively, to obtain polluted seawater.

[0029] C. Under natural light and room temperature, put the Oyster near river treated in step A into the polluted seawater obtained in step B for temporary cultivation. No bait is thrown during the temporary cultivation, and continuous aeration is carried out day and night to ensure sufficient dissolved oxygen in the water. Change the seawater every other day and clean out the dead oysters. After 10 days of exposure, take out all the remaining oysters from the pool, remove the shells, take out the soft tissues and suck the water with filter paper, and g...

Embodiment 2

[0059] The precision of the inventive method:

[0060] (1) National standard method processing, ICP detection, the specific process is as follows:

[0061] According to embodiment step (1), prepare contaminated sample, obtain the sample before standard addition, the sample before standard addition detects according to the following steps, obtain the heavy metal content (as C of table 4) before sample standard addition 0 value shown). To the sample before the standard addition, the amount of the added standard is shown in the C value of Table 4, and the sample after the standard addition is detected according to the following steps to obtain the heavy metal content of the sample after the standard addition (as shown in the C value of Table 4). 1 value shown). According to formula 1, the standard recovery rate W of the sample was obtained.

[0062] W(%)=(C 1 -C 0 ) / C×100% (Formula 1)

[0063] formula,

[0064] W: the standard recovery rate of the sample (%);

[0065] C ...

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Abstract

The invention discloses a method for leaching heavy metals in protein foods by using diluted acid and application thereof. The method disclosed by the invention specifically comprises the steps of crushing the protein foods, adding the diluted acid to leach the protein foods, then removing insoluble substances, and ultrafiltering and concentrating the protein foods to obtain a solution containingheavy metal ions. The method disclosed by the invention can be used for leaching heavy metal ions of copper, lead and cadmium in aquatic products and agricultural product livestock tissues so that the extracting time for leaching the heavy metals from a sample is shortened and the pollution brought by the traditional sample pretreatment is also reduced. Compared with a resolution method in a standard in China, the method disclosed by the invention has the advantage of mild reaction condition; and the obtained leaching solution can be combined with an enzyme-linked immunosorbent assay method to realize a field fast detection technology of agricultural products. Therefore, the method for leaching the heavy metals in the protein foods by using the diluted acid, disclosed by the invention, can be conveniently and rapidly applied as a sample pretreatment technology for detecting the heavy metals by using an enzyme-linked immunosorbent assay technology.

Description

technical field [0001] The invention relates to a method for extracting heavy metals, in particular to a method and application of dilute acid for extracting heavy metals in protein foods. This method is used as a rapid and efficient sample pretreatment technology for the detection of heavy metals in agricultural and aquatic products by ELISA. Background technique [0002] With the acceleration of urbanization and industrialization, the discharge of urban domestic waste and industrial "three wastes" is increasing, and heavy metal pollutants are constantly accumulating and amplified through the food chain, making the heavy metals in agricultural products and aquatic products exceed the standard, and the content of heavy metals in food Agricultural products and aquatic products that exceed the standard will cause certain harm to the human body, and the monitoring and prevention of heavy metal pollution has become a common concern of all countries in the world. [0003] Heavy ...

Claims

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Application Information

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IPC IPC(8): A23L1/015A23L5/20
Inventor 江天久汪慧唐勇
Owner JINAN UNIVERSITY
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