Cell blending apparatus for MTT colorimetric test

A cell and experimental technology, which is applied in the preparation of test samples, mixers with rotating stirring devices, mixers, etc., can solve problems such as uneven distribution of cells in each hole, differences in cell concentration, and influence on experimental results, so as to prevent Aggregation, prevention of cross-contamination, uniform mixing effect

Inactive Publication Date: 2011-11-23
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

During this process, due to the difference in strength or times of blowing, the concentration of cells is different, resulting in uneven

Method used

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  • Cell blending apparatus for MTT colorimetric test
  • Cell blending apparatus for MTT colorimetric test

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Example Embodiment

[0009] like figure 1 As shown, a cell mixing device for MTT colorimetric experiments includes a metal container 9, a constant flow pump 1; a comprehensive fixing frame 14, and a liquid taking tank 3 with liquid inlet and outlet. The constant flow pump 1, the metal container 9 and the liquid taking tank 3 are connected by pipelines to form a cell fluid circulation system.

[0010] The metal container 9 is an oxidation-resistant and corrosion-resistant cylindrical container, and two hoops 8 are arranged on its circumference for fixing it in the comprehensive fixing frame 14 . The bottom of the metal container 9 is provided with an L-shaped metal lower elbow 10, the mouth of which protrudes 1 cm from the bottom of the container, and connects the catheter 12, which is connected to the liquid inlet of the liquid extraction tank 3 after passing through the constant flow pump 1 . Metal container 9 tops are provided with a detachable end cap, and a rotor is inlaid in the center of e...

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Abstract

The invention discloses a cell blending apparatus for an MTT colorimetric test. The apparatus is characterized in that: the apparatus comprises a constant flow pump, a metal container, and a liquid taking pot with a liquid inlet and a liquid outlet, the metal container and the constant flow pump are paratactically and tightly fixed in an comprehensive mount, the a liquid taking pot is tightly fixed above a space between the metal container and the constant flow pump in the comprehensive mount; the bottom of the metal container is provided with a lower bend tube for connecting a catheter, and the catheter is connected with the liquid inlet of the liquid taking pot after passing through the constant flow pump; and the top of the metal container is provided with a detachable end cover, the center of the end cover is provided with a stirrer with a driven wheel extruding into the metal container, a part of the end cover having a distance from the center of the end cover more than the radius of the driven wheel is provided with a liquid feeding aperture with an aperture cover, a position of the end cover with the diameter direction opposite to the liquid feeding aperture is provided with an upper bend tube, the upper bend tube is connected with the liquid outlet of the liquid taking pot through a flexible tube, and the central shaft of the constant flow pump is provided with two driving wheels, wherein the upper driving wheel is connected with the driven wheel on the end cover of the metal container through a conveyor belt.

Description

technical field [0001] The invention relates to a cell mixing device used in MTT (thiazolium blue) colorimetric experiments in medical teaching and scientific research. Background technique [0002] In recent years, with the deepening and development of the field of medical research, cytology research is one of the important links in the field of medical research. The reason is that cells are the most basic unit of the structure and life activities of living organisms. MTT colorimetric assay is a detection method that indirectly reflects cell proliferation by measuring the energy metabolism level of cells. The detection principle is that succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-purple crystal formazan (Formazan) and deposit in the cells, while dead cells have no such function. Dimethyl sulfoxide (DMSO) can dissolve formazan in cells, and its light absorption value is measured at the corresponding wavelength...

Claims

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Application Information

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IPC IPC(8): B01F7/00B01F3/08G01N1/38G01N21/31
Inventor 曲萍于军赵海康张学策南海燕颜林枫仝黎赵玉峰刘芳娥田菲刘楠楠梁向艳黄小军铁茹
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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