Cell blending apparatus for MTT colorimetric test

Abstract

The invention discloses a cell blending apparatus for an MTT colorimetric test. The apparatus is characterized in that: the apparatus comprises a constant flow pump, a metal container, and a liquid taking pot with a liquid inlet and a liquid outlet, the metal container and the constant flow pump are paratactically and tightly fixed in an comprehensive mount, the a liquid taking pot is tightly fixed above a space between the metal container and the constant flow pump in the comprehensive mount; the bottom of the metal container is provided with a lower bend tube for connecting a catheter, and the catheter is connected with the liquid inlet of the liquid taking pot after passing through the constant flow pump; and the top of the metal container is provided with a detachable end cover, the center of the end cover is provided with a stirrer with a driven wheel extruding into the metal container, a part of the end cover having a distance from the center of the end cover more than the radius of the driven wheel is provided with a liquid feeding aperture with an aperture cover, a position of the end cover with the diameter direction opposite to the liquid feeding aperture is provided with an upper bend tube, the upper bend tube is connected with the liquid outlet of the liquid taking pot through a flexible tube, and the central shaft of the constant flow pump is provided with two driving wheels, wherein the upper driving wheel is connected with the driven wheel on the end cover of the metal container through a conveyor belt.

Description

technical field [0001] The invention relates to a cell mixing device used in MTT (thiazolium blue) colorimetric experiments in medical teaching and scientific research. Background technique [0002] In recent years, with the deepening and development of the field of medical research, cytology research is one of the important links in the field of medical research. The reason is that cells are the most basic unit of the structure and life activities of living organisms. MTT colorimetric assay is a detection method that indirectly reflects cell proliferation by measuring the energy metabolism level of cells. The detection principle is that succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-purple crystal formazan (Formazan) and deposit in the cells, while dead cells have no such function. Dimethyl sulfoxide (DMSO) can dissolve formazan in cells, and its light absorption value is measured at the corresponding wavelength...

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Problems solved by technology

During this process, due to the difference in strength or times of blowing, the concentration of cells is different, resulting in uneven distribution of cells in each well and affecting the experimental results. This problem has troubled most experimenters for a long time.

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