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Method for extracting refined cottonseed flavonoids from cottonseed hulls

A cottonseed hull and flavonoid technology, which is applied in pharmaceutical formulations, medical preparations containing active ingredients, and plant raw materials, etc., can solve the problems of large investment in equipment for ultrasonic extraction, high cost of repeated solvent extraction, and low extraction rate by alcohol extraction. problem, to achieve the effect of reducing extraction cost, low cost and low production cost

Active Publication Date: 2011-12-07
邯郸晨光植物蛋白有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the cost of repeated solvent extraction is high, and the extraction rate of alcohol extraction is low, resulting in high cost. Ultrasonic extraction has a large investment in equipment and has not yet been industrialized.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take 100 kilograms of dry cottonseed hulls and crush them to 30-40 meshes, put them into 600 kilograms of 0.3 mol / L HCl solution, add 600 kilograms of KOH solution with a mass fraction of 0.5% to the cottonseed hulls, heat to 45°C and soak After 3 hours, release the solution; then add 600 kg of KOH solution with a mass fraction of 0.5% to the cottonseed hulls, soak at 45°C for 3 hours, and release the solution again; then add 600 kg of KOH with a mass fraction of 0.5% to the cottonseed hulls again solution, heated to 45°C and soaked for 3 hours, released the solution; combined the obtained solutions (water extracts), cooled and centrifuged to remove mechanical impurities; adjusted the pH value of the supernatant to 2.0 with hydrochloric acid, passed through a macroporous resin column Purified, analyzed with 50% ethanol solution by volume, concentrated and spray-dried to obtain 2.87 kg of cottonseed flavonoids. The total flavonoids in cottonseed were detected and calcula...

Embodiment 2

[0021] Take 100 kilograms of dry cottonseed hulls and crush them to 30-40 meshes, put them into 300 kilograms of 0.2mol / L sulfuric acid solution, add 300 kilograms of NaOH solution with a mass fraction of 1.0% to the cottonseed hulls, heat to 45°C and soak After 3 hours, release the solution; then add 300 kg of NaOH solution with a mass fraction of 1.0% to the cottonseed hulls, soak at 45°C for 3 hours, and release the aqueous solution again; then add 300 kg of NaOH solution with a mass fraction of 1.0% to the cottonseed hulls again , heated to 45°C and soaked for 3 hours, released the solution; combined the obtained aqueous solutions (water extracts), cooled and centrifuged to remove mechanical impurities; adjusted the pH value of the supernatant to 1.0 with sulfuric acid, and purified it through a macroporous resin column , the volume percent concentration is 50% ethanol solution analysis, concentration and spray drying to obtain 2.73 kg of cottonseed flavonoids. The total f...

Embodiment 3

[0023] Take 100 kilograms of dried cottonseed hulls and crush them to 30-40 meshes, put them into 450 kilograms of 0.5mol / L hydrochloric acid solution and phosphoric acid solution (weight ratio 1:1), filter and add 600 kilograms to the cottonseed hulls with a mass fraction of 0.3 % ammonia solution, heated to 45°C for 3 hours, and then released the solution; then added 600 kg of ammonia solution with a mass fraction of 0.3% to the cottonseed hulls, soaked at 45°C for 3 hours, and released the aqueous solution again; then added to the cottonseed hulls again 600 kg of ammonia solution with a mass fraction of 0.3%, heated to 45°C and soaked for 3 hours, released the solution; combined the obtained aqueous solutions (water extracts), cooled and centrifuged to remove mechanical impurities; adjusted the pH of the supernatant with phosphoric acid After reaching 4.0, it was purified by a macroporous resin column, analyzed with a volume percent concentration of 50% methanol solution, co...

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Abstract

The invention discloses a method for extracting and refining cottonseed flavone from cottonseed shells, and the method comprises the process steps: (1) crushing dried cottonseed shells to 30-40 meshes; (2) soaking the crushed cottonseed shells in 0.2-0.5 mol / L acid solution for 1-3 hours and filtering out filtrate; (3) after the filtrate is filtered out, extracting flavone from the cottonseed shells by using an alkaline solution, and centrifuging the obtained water extract to remove impurities; and (4) regulating the pH value of the supernatant obtained by centrifugation to 1-4 by using acid,purifying the flavone with a macroporous resin column, and desorbing with methanol, ethanol or propanol solution to obtain the cottonseed flavone. In the method, the alkaline solution is as an extractant, the required equipment is simple, the use amount of the solvent is reduced, and low-cost common acids and bases are utilized, thus effectively reducing the extraction cost of the flavone in the cottonseed, simultaneously reducing environmental pollution and improving operation safety. The method has the advantages of simple process, and lower production cost, and is suitable for industrial production, moreover the prepared product has good quality.

Description

technical field [0001] The invention relates to an industrial method for purifying natural extracts, in particular to a method for extracting refined cottonseed flavonoids from cottonseed hulls. Background technique [0002] The main components of cottonseed flavonoids are flavonol glycosides, which are quercetin-3-O apiose-(1→2)-rhamnose-(1→6)-glucoside and kaempferen-3-O apiose-(1 →2)-Rhamnose-(1→6)-glucoside conjugates. The main function is to relax coronary artery, improve microcirculation, antithrombotic, have protective effect on cardiovascular and cerebrovascular, and have antidepressant active ingredients. [0003] At present, the production processes of cottonseed flavonoids mainly include repeated solvent extraction, alcohol extraction and ultrasonic extraction. Among them, the cost of repeated solvent extraction is high, and the extraction rate of alcohol extraction is low, resulting in high cost. Ultrasonic extraction has a large investment in equipment and has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P7/02A61P9/10A61P25/24
Inventor 卢庆国连运河程远欣丁红梅
Owner 邯郸晨光植物蛋白有限公司