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Method for preparing mannan-oligosaccharide by using waste beer yeast

A technology of beer waste yeast and mannan oligosaccharides, which is applied in the field of feed additives, can solve the problems of high production costs, and achieve the effects of increasing added value, good stability, and zero pollution

Inactive Publication Date: 2013-07-03
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the existing problem of high production cost of preparing mannan by hydrolyzing plant polysaccharides with β-mannanase, the purpose of the present invention is to provide a method for producing isomannan by using beer waste yeast, and combining isomannan Method for preparing mannan oligosaccharide by hydrolysis with enzyme

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: The method for preparing mannan oligosaccharides using spent brewer's yeast of the present invention comprises the following steps:

[0032] 1. Preparation of Yeast Cell Wall

[0033] 1) Pretreatment of waste beer yeast: Take the waste beer yeast out of the fermentation tank and wash it with tap water. The water temperature generally does not exceed 10°C to prevent autolysis of the yeast. Centrifuge the washed waste yeast to obtain yeast sludge, centrifuge The rotating speed of the machine is 3000r / min, and the centrifugation time is 30min. The treated yeast sludge is off-white and creamy and stored at low temperature.

[0034] 2) Preparation of yeast liquid: Mix acetic acid with mass concentration of 2.2g / L~2.6g / L, sodium acetate with mass concentration of 99.2g / L~119.2g / , sodium chloride with concentration of 10g / L~30g / L and distilled water to prepare The acetic acid-sodium acetate buffer solution is obtained, and the pH value of the acetic acid-sodium ...

Embodiment 2

[0044] Embodiment 2: the preparation of 1kg mannan oligosaccharide

[0045] Take 1kg of beer waste yeast, add 4.375L of distilled water and 5.625L of acetic acid-sodium acetate buffer (pH=6.0) to it, and centrifuge the mixture at a temperature of 50°C in a water bath for 24 hours at a speed of 3000r / min. The centrifugation time was 10 min, the supernatant was discarded, the precipitated part was transferred to a petri dish and placed in an oven, and dried at 60° C. for 24 h to obtain a yeast cell wall solid. Get 1 kg of yeast cell wall solids, add 6.875 L of isomannanase crude enzyme solution and 5.625 L of distilled water, adjust the pH value to 6.0, and at a temperature of 55 ° C, take out and dry after hydrolyzing in a water bath for 4.5 h, and pulverize to obtain a weight of 1kg of mannan oligosaccharides, the effective content of mannan oligosaccharides is 6.06%.

[0046] Embodiment 2: the preparation of 1000kg mannan oligosaccharide

[0047] Take 1000kg of beer waste y...

Embodiment 3

[0048] Embodiment 3: Preparation of 20 tons of mannan oligosaccharides

[0049] Take 25 tons of beer waste yeast, add 87500L of distilled water and 112500L of acetic acid-sodium acetate buffer (pH=6.0) to it, and centrifuge the mixture at a temperature of 50°C in a water bath for 24 hours at a speed of 3000r / min. The time was 10 minutes, the supernatant was discarded, the precipitated part was transferred to an oven, and dried at 60° C. for 24 hours to obtain yeast cell wall solids. Get 20 tons of yeast cell wall solids, add 137,500 L of isomannanase crude enzyme solution and 112,500 L of distilled water, adjust the pH value to 6.0, and at a temperature of 55 ° C, take out and dry after hydrolyzing in a water bath for 4.5 hours, and pulverize to obtain a weight of 20 tons of mannan oligosaccharides.

[0050] This product that utilizes above-mentioned technology to obtain is used as feed additive, is added in the feed of Oni tilapia with the consumption of 5g / kg, and the weigh...

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Abstract

The invention discloses a method for preparing mannan-oligosaccharide by using waste beer yeast, and relates to the technical field of feed additives. The invention aims to solve the problem of high production cost in the conventional method for preparing the mannan-oligosaccharide by hydrolyzing vegetable polysaccharide through beta-mannase. The preparation method comprises the following steps of: (1) preparing the waste beer yeast into a yeast cell wall liquid or solid; (2) preparing a crude enzyme liquid of iso-mannase; and (3) adding the crude enzyme liquid of iso-mannase prepared in the step (2) into the yeast cell wall liquid or solid prepared in the step (1), and hydrolyzing to obtain the mannan-oligosaccharide. The method is used for producing the mannan-oligosaccharide serving as an animal and aquatic feed additive.

Description

technical field [0001] The invention relates to the technical field of feed additives, in particular to a method for preparing mannan oligosaccharides by autolyzing spent beer yeast and adding microbial enzymes. Background technique [0002] my country's beer industry has developed rapidly in recent decades, and there are currently more than 800 beer manufacturers. Since 2001, my country has become the world's largest beer producing country, and beer production is still growing at a rate of 5% to 7% every year. In 2010, the production volume of beer reached 44.83 million tons. Beer waste yeast is an important by-product in beer production, and the output of yeast sludge accounts for about 2% to 3% of the total beer output. Every year, my country's beer production enterprises discard as many as 900,000 tons to 1.35 million tons of waste yeast sludge. The main components of spent beer yeast are proteins, polysaccharides, nucleic acids, vitamins, trace elements and enzymes, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12R1/125C12R1/085
Inventor 汪立平穆昭艳冷向军赵勇
Owner SHANGHAI OCEAN UNIV