Stem cell combined growth factor injection for promoting angiogenesis in ischemic tissue and its preparation method and use method

Abstract

The invention relates to a stem cell combined growth factor injection for promoting angiogenesis in ischemic tissue, a preparation method and a use method thereof. The body’s protective response to peripheral tissue ischemia is relatively complex. Although a single growth factor intervention is effective, it cannot complete this process; although stem and progenitor cell transplantation can improve the vascular density and blood perfusion of ischemic tissue, the main source of cells is bone marrow. There are serious limitations in the acquisition of umbilical cord blood, umbilical cord blood, etc., and the in vivo viability of cells is extremely limited, which seriously affects its therapeutic effect. The invention utilizes human basic fibroblast growth factor to intervene in adipose mesenchymal stromal cells, and cooperates with vascular endothelial cell growth factor to promote angiogenesis in ischemic tissue. The present invention overcomes the benefit limitation of single factor or cell therapy, ensures sufficient stem cell acquisition, and improves the survival ability and angiogenesis effect of transplanted cells, thereby further improving blood perfusion of ischemic lower limbs and improving limb survival Rate.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a stem cell combined growth factor injection for promoting angiogenesis in ischemic tissue and a preparation method and application method thereof. Background technique [0002] The prevalence of peripheral arterial disease (PAD) in China is high. Patients with severe PAD often lose the indications for interventional or surgical revascularization. Drug treatment is difficult to change the natural history of PAD, which eventually leads to poor prognosis such as amputation. Studies have shown that growth factors, bone marrow-derived stem cells and progenitor cells have therapeutic angiogenesis effects, can promote angiogenesis in ischemic tissue, and improve blood perfusion, that is, "biological arterial bypass". [0003] However, the effect of existing biological treatment methods using a single gene or growth factor in the treatment of peripheral arterial di...

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Problems solved by technology

[0003] However, the effect of existing biological treatment methods using a single gene or growth factor in the treatment of peripheral arterial disease is inaccurate. One of the possible reasons is that the body's protective response to peripheral tissue ischemia is relatively complex. Although a single growth factor intervention is effective, it cannot complete this task. One process; although stem and progenitor cell transplantation can improve the vascular density and blood perfusion of ischemic tissue, there are serious limitations in obtaining the main cell sources such as bone marrow and umbilical cord blood, and the viability of cells in vivo is extremely limited, which seriously affects other The role of treatment

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