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Composition comprising mesenchymal stem cells or mesenchymal stem cell culture fluid for preventing or treating neurological diseases

一种质干细胞、神经疾病的技术，应用在阿尔茨海默氏病的组合物,疾病的组合物领域，能够解决昂贵工作、骨髓捐献耗时、痛苦等问题，达到防止蛋白磷酸化、防止神经突损伤、防止神经细胞毒性的效果

Inactive Publication Date: 2011-12-14
MEDIPOST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the collection of bone marrow-derived MSCs requires various complicated stages of medical treatment, making bone marrow donation a time-consuming, psychologically and physically painful, and expensive endeavor

Method used

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  • Composition comprising mesenchymal stem cells or mesenchymal stem cell culture fluid for preventing or treating neurological diseases
  • Composition comprising mesenchymal stem cells or mesenchymal stem cell culture fluid for preventing or treating neurological diseases
  • Composition comprising mesenchymal stem cells or mesenchymal stem cell culture fluid for preventing or treating neurological diseases

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0130] Example 1: Isolation and culture of neural stem cells

[0131] Neural stem cells used herein were isolated as follows. Neural stem cells were obtained from the cerebral cortex and hippocampus of 14-day Sprague-Dawley rats (Orient Bion Inc., Korea) embryos (embryonic day 14, E14) Separated to get. First, cut the abdomen of the pregnant rat and separate the embryos using scissors and forceps. Embryos were detached by washing with Hank's Balanced Salt Solution (HBSS) and placed in a Petri dish containing ice-cold HBSS. Under a microscope, separate the cerebral cortex and hippocampus of E14 embryos using a needle and forceps. Use a pipette to aspirate the isolated cerebral cortex 10 to 20 times to single cells and place in serum-free medium. Single cells were treated with poly-L-ornithine (15 μg / mL; Sigma, St. Louis, MO) for 16 hours at 37°C and spread onto cells coated with fibronectin ( 1 µg / ml; Sigma) for at least 2 hours. In serum-free Neurobasal supplemented with...

example 2

[0132] Example 2: Isolation and expansion of UCB-derived MSCs

[0133] Immediately after birth, umbilical cord blood (UCB) samples were collected from the umbilical vein with the mother's permission. Specifically, using a UCB collection bag connected to 44 ml of citratephosphate dextrose anticoagulant-1 (CPDA-1) anticoagulant (GreenCross Corp., Korea) The umbilical vein was pricked with a 16-gauge needle to collect the UCB into a collection bag under gravity. After collection, the UCB thus obtained was processed within 48 hours, and the survival rate of monocytes exceeded 90%. The collected UCB was centrifuged using a Ficoll-diatrizoate gradient (density: 1.077 g / ml; Sigma) to obtain mononuclear cells, which were washed several times to remove impurities. Cells were suspended in Minimal Essential Medium (α-MEM; Gibco BRL) supplemented with 10% to 20% FBS (HyClone). Introduce cells to optimal concentration in Minimal Essential Medium supplemented with 10% to 20% FBS and grow...

example 3

[0134] Example 3: Toxicity of amyloid beta protein

[0135] To prepare for ideal Alzheimer's disease onset conditions, amyloid beta protein fragment 1-42 (Aβ42, Sigma, Inc. (Sigma, Inc.) sigma), A9810) serum-free Neurobasal TM Neurons differentiated as described in Example 1 were cultured in culture medium. After the neural stem cells were differentiated for 3 to 4 days, the morphological characteristics of the neural stem cells were observed using a microscope. If differentiation into neurons was identified, cells were treated with Aβ for 24 hours.

[0136] figure 1 Light microscopy images illustrating untreated and amyloid-β-treated live neurons for 24 hours, which were used to measure morphological changes in neurons. As the concentration of amyloid-beta increased, the number of neurons that died increased. exist figure 1 In, the control group showed that in serum-free Neurobasal without amyloid-beta protein TM Neurons cultured in media with Aβ-1 micromolar, Aβ-5 m...

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Abstract

Provided are a pharmaceutical composition for prevention and treatment of a neural disease including at least one selected from the group consisting of mesenchymal stem cells (MSCs), a culture solution of the MSCs, activin A, PF4, decorin, galectin 3, GDF15, glypican 3, MFRP, ICAM5, IGFBP7, PDGF-AA, SPARCL1, thrombospondin-1, WISP1, progranulin, IL-4, a factor inducing expression thereof, and any combination thereof, and a method therefor.

Description

technical field [0001] The present invention relates to a composition for preventing or treating Alzheimer's disease (Alzheimer's disease) related to neurite damage, which comprises mesenchymal stem cells (mesenchymal stem cell, MSC), MSC culture fluid, The protein contained in the MSC culture solution or the signal transduction system stimulating factor that induces the expression of the protein. [0002] The present invention relates to a composition for preventing or treating diseases related to neurite damage, which comprises mesenchymal stem cells (MSC), MSC culture fluid, a protein contained in the MSC culture fluid, or a signal that induces the expression of the protein Transducer system stimulator. Background technique [0003] Alzheimer's disease, a brain disorder that destroys brain cells caused by the destructive build-up of amyloid-beta protein and generally occurs with age, is a serious disease that causes language impairment and impaired cognitive function. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/44A61K35/28A61P25/00A61P25/28A61P25/16
CPCC12N2502/137C12N5/0619C12N2502/1358C12N5/0665A61K38/2026A61K35/28A61P25/00A61P25/02A61P25/08A61P25/16A61P25/18A61P25/24A61P25/28A61K35/50
Inventor 梁允瑄吴元一蒋锺旭金珠渊
Owner MEDIPOST
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