Method for preparing rice field eel protein from rice field eel leftovers

A technology for leftovers and eel, which is applied in the field of preparing eel protein, can solve problems such as environmental pollution, and achieve the effects of low molecular weight, simple and safe production steps, and easy control.

Inactive Publication Date: 2011-12-21
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, a large amount of leftovers such as eel heads, eel tails, and backbones are produced, and most of them are treated as waste, which greatly pollutes the environment

Method used

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  • Method for preparing rice field eel protein from rice field eel leftovers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Preparation of eel protein according to the following steps

[0020] (1) Wash 50kg of eel scraps with clean water, pulverize to 40 mesh, add 100kg of water, heat to 65°C, and hold for 30 minutes;

[0021] (2) Lower the temperature to 60-65°C, adjust the pH to 8.5, add bacterial alkaline protease Protex 6L to control the enzyme concentration at 1000U / L, keep the enzyme hydrolyzed at 60°C for 8 h, and then heat up to 80-85°C after the end of the enzymatic hydrolysis. ℃, keep warm for 10 min;

[0022] (3) the temperature is lowered to 50°C, and the pH of the enzymatic hydrolysis solution is adjusted to 10.0 with a mass concentration of 95% NaOH solution;

[0023] (4) Filter the enzymatic hydrolysis solution in step 3 to remove the bone residue to obtain a supernatant;

[0024] (5) Desalting and concentrating the supernatant of step 4 with a membrane with a molecular weight cut-off of 1k;

[0025] (6) Add 1.5kg of activated carbon to the concentrate in step...

Embodiment 2

[0028] Example 2: Preparation of eel protein according to the following steps

[0029] (1) Wash 50kg of eel scraps with clean water, pulverize to 40 mesh, add 150kg of water, heat to 75°C, and keep for 20 minutes;

[0030] (2) Lower the temperature to 60-65°C, adjust the pH to 9.5, add bacterial alkaline protease Protex 6L to control the enzyme concentration at 1500U / L, keep the temperature at 60°C for enzymatic hydrolysis for 6 hours, and then heat up to 80-85°C after the enzymatic hydrolysis is completed. , keep warm for 8 min;

[0031] (3) reduce the temperature to 55°C, adjust the pH of the enzymatic hydrolysis solution to 10.0 with a 95% NaOH solution by mass concentration;

[0032] (4) Filter the enzymatic hydrolysis solution in step 3 to remove the bone residue to obtain a supernatant;

[0033] (5) Desalting and concentrating the supernatant of step 4 with a membrane with a molecular weight cut-off of 1k;

[0034] (6) Add 2.5kg activated carbon to the concentrated so...

Embodiment 3

[0037] Example 3: Preparation of eel protein according to the following steps

[0038] (1) Wash 50kg of eel scraps with clean water, pulverize to 40 mesh, add 200kg of water, heat to 85°C, and hold for 10 minutes;

[0039] (2) Lower the temperature to 60-65°C, adjust the pH to 10.0, add bacterial alkaline protease Protex 6L to control the enzyme concentration at 2000U / L, keep the temperature at 60°C for enzymatic hydrolysis for 5 hours, and then heat up to 80-85°C after enzymatic hydrolysis. ℃, hold for 5 min;

[0040] (3) the temperature is lowered to 60°C, and the pH of the enzymatic hydrolysis solution is adjusted to 10.0 with a 95% mass concentration NaOH solution;

[0041] (4) Filter the enzymatic hydrolysis solution in step 3 to remove the bone residue to obtain a supernatant;

[0042] (5) Desalting and concentrating the supernatant of step 4 with a membrane with a molecular weight cut-off of 1k;

[0043] (6) Add 3.0kg of activated carbon to the concentrated soluti...

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PUM

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Abstract

The invention discloses a method for preparing rice field eel protein from rice field eel leftovers. The method utilizes the specificity of the enzyme to efficiently hydrolyze the rice field eel wastes, and then obtains them through filtration of enzyme degradation products, membrane separation and concentration, decolorization and deodorization, and spray drying. Eel protein. The invention provides a production method of rice field eel protein which fully utilizes rice field eel leftovers to turn waste into treasure, has simple and safe steps, and is easy to operate. The effect is very remarkable and is suitable for industrialized production.

Description

technical field [0001] The invention relates to a method for preparing rice field eel protein from rice field eel leftovers, and belongs to the technical field of biological extraction. Background technique [0002] Field eels belong to the order Synbranchidae, Synbranchidae, and the genus Monopterus. Commonly known as: eel, long fish. Swamp eel is widely distributed in my country and is an important and valuable freshwater economic fish. According to the "Compendium of Materia Medica", rice field eel has the effects of nourishing blood, nourishing qi, reducing inflammation, disinfecting, and removing rheumatism. Rice field eel meat is sweet and warm in nature, has the effect of nourishing the middle and blood, and curing deficiency. It is used in folk medicine to treat diseases such as consumptive cough, damp and hot body itching, intestinal wind hemorrhoids, and deafness. Every 100 grams of eel meat contains 17.2-18.8 grams of protein, 0.9-1.2 grams of fat, 38 mg of cal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07K1/14
Inventor 陈晓明李松林叶华白青云
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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