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A quantitative detection method for uric acid based on surface-enhanced Raman spectroscopy

A surface-enhanced Raman, quantitative detection method technology, applied in Raman scattering, material excitation analysis and other directions, can solve problems such as blanks, and achieve accurate quantitative detection, measurement and analysis in a short time and less sample consumption.

Inactive Publication Date: 2011-12-21
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Quantitative detection of uric acid by surface-enhanced Raman spectroscopy has not been reported at home and abroad, and this field is still blank

Method used

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  • A quantitative detection method for uric acid based on surface-enhanced Raman spectroscopy
  • A quantitative detection method for uric acid based on surface-enhanced Raman spectroscopy
  • A quantitative detection method for uric acid based on surface-enhanced Raman spectroscopy

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Preparation of SERS active substrate-silver colloid: Add a total of 4.5mL of NaOH solution (0.1M / L) to 4.5mL of hydroxylamine hydrochloride (6×10 -2 M / L), then quickly add the mixture to 81mL AgNO 3 (1.11×10 -3 M / L), shake until a uniform milk gray silver colloid solution is obtained.

[0031] 2. Centrifuge the silver colloid at 10,000r / min for 10 minutes, and remove the lower layer of high-concentration silver colloid for later use.

[0032] 3. Accurately weigh 0.0250g of uric acid, dissolve it, transfer it to a 250mL volumetric flask, and make it into a 100mg / L stock solution. Pipette 80.00mL of the stock solution into a 100mL volumetric flask, add hydrochloric acid solution to adjust the pH to 5.8, 7.5, and 10.7, and constant volume to prepare 80mg / L uric acid solution. figure 2 Curves A, B, and C in the middle are the SERS spectra of solutions with the same concentration at pH=5.8, 7.5, and 10.7, respectively. It can be seen that the SERS spectrum signal is ...

Embodiment 2

[0042] 1. Preparation of SERS active substrate-silver colloid: add a total of 4.5mL of NaOH solution (0.1M / L) to 5.8mL of hydroxylamine hydrochloride (6×10 -2 M / L), then quickly add the mixture to 100mL AgNO 3 (1.11×10 -3 M / L), shake until a uniform milk gray silver colloid solution is obtained.

[0043] 2. Centrifuge the silver colloid at 10,000r / min for 10 minutes, and remove the lower layer of high-concentration silver colloid for later use.

[0044] 3. Preparation of alkaline uric acid solution: Accurately weigh 0.02 g of uric acid, and use 11.6 mmol / L Na 2 CO 3 solution at 70 0 Dissolve in a water bath and dilute to a 100mL volumetric flask, dilute this solution in turn, and prepare uric acid standards with 5 different gradient concentrations of 20mg / L, 40mg / L, 70mg / L, 100mg / L, and 140mg / L Solution samples.

[0045] 4. Mix 20 μL of uric acid standard solutions of different concentrations prepared in step 3 with an equal volume of high-concentration silver colloid on...

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Abstract

The invention relates to a method for the quantitative detection of uric acid based on surface enhanced Raman spectroscopy (SERS). The method comprises the following steps of: adding NaOH and hydroxylamine hydrochloride into an AgNO3 solution to obtain silver colloid; centrifuging the silver colloid to obtain high-concentration silver colloid; mixing uric acid solutions of different concentrations with the high-concentration silver colloid and adding a K2SO4 solution to perform a Raman spectroscopy test to obtain a uric acid SERS spectrum; testing the high-concentration silver colloid to obtain a silver colloid background SERS spectrogram; performing intensity normalization by using a silver colloid background Raman signal as internal standard, establishing a relative intensity-concentration standard working curve diagram of uric acid SERS spectral line; and deducing uric acid concentration by comparing the SERS spectrogram of the uric acid with unknown concentration with the relativeintensity-concentration standard working curve diagram of uric acid SERS spectrum. The method provided by the invention can be used for solving the problems that low-concentration uric acid solution has weak Raman signal and can be interfered with other impurities easily, obtaining high-quality uric acid SERS spectrum and realizing the quantitative detection of uric acid by means of the SERS spectrum.

Description

technical field [0001] The invention relates to a quantitative detection method of uric acid, specifically a method for quantitative detection of uric acid concentration by using surface-enhanced Raman spectroscopy (SERS for short), and belongs to the technical field of drug and drug detection. Background technique [0002] Surface-enhanced Raman scattering was first discovered by Fleischmann et al. in 1974 when they conducted electrochemical experiments to study the Raman scattering experiments of pyridine molecules adsorbed on the surface of rough silver electrodes. The surface-enhanced Raman scattering effect refers to the phenomenon that when the target molecule is adsorbed on some rough noble metal good conductor surfaces or sols, its Raman scattering signal is greatly enhanced compared with the conventional Raman spectrum signal. Surface-enhanced Raman spectroscopy (SERS) technology is widely used in surface research, interface orientation and configuration of biologic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 冯尚源陈荣林多林居强李永增黄祖芳陈燕坪俞允席刚琴
Owner FUJIAN NORMAL UNIV
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