A method for measuring hydroxyl radicals in plants
A measurement method and a technology of free radicals, applied in the direction of analysis by electron paramagnetic resonance, etc., can solve the problems of many interference factors, achieve high sensitivity, high efficiency detection, and strong anti-interference ability of plant matrix
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Embodiment 1
[0046] Example 1: Determination of hydroxyl radicals in cadmium-stressed corn roots using electron spin resonance combined with secondary free radical spin trapping
[0047] Soak the roots of corn seedlings 5 days after germination in 5 mg / L Cd(NO 3 ) 2 (purchased from: Beijing Chemical Reagent Company) aqueous solution for 24 hours, separate the roots and leaves of the seedlings, and wash the roots and leaves with tap water and distilled water respectively. Weigh 0.2-1.5g of fresh plant roots, cut them into pieces and place them in a glass tissue grinder, add 0.5-1.5ml of 10-100mM PBN in DMSO solution, and grind them evenly in an ice bath. Transfer the grinding solution to a centrifuge tube with a volume of 1.5ml (purchased from: Cangxian Dusheng Qiangfa Medical Instrument Factory, Hebei Province), and after warming in a water bath at 20-45°C for 5-20 minutes, place the centrifuge tube in a refrigerated centrifuge (Model: GL-8MS, purchased from: Shanghai Luxiangyi Centrif...
Embodiment 2
[0049] Example 2: Determination of hydroxyl radicals in wheat roots and leaves under atrazine stress by electron spin resonance combined with secondary free radical spin capture technology
[0050] Roots of wheat seedlings 5 days after germination were soaked in 10 mg / L atrazine (purchased from: Sigma-Aldrich Company). After 24 hours, the roots and leaves of the seedlings were separated, and the roots and leaves were washed with tap water and distilled water, respectively. Weigh 0.2-1.5g of fresh plant roots or leaves, cut them into pieces and place them in a glass tissue grinder, add 0.5-1.5ml of 10-100mM PBN in DMSO solution, and grind them evenly in an ice bath. Transfer the grinding solution to a centrifuge tube, warm it in a water bath at 20-45°C for 5-20 minutes, and then place the centrifuge tube in a refrigerated centrifuge (model: GL-8MS, purchased from: Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.) In refrigerated centrifugation at 2000-6000g for 3-10 minute...
Embodiment 3
[0052] Example 3: Determination of hydroxyl radicals in corn roots under different concentrations of aureomycin stress by electron spin resonance combined with secondary free radical spin capture technology
[0053] Soak the roots of corn seedlings 5 days after germination in 0, 0.5, 5, and 50 mg / L aureomycin (CTC, purchased from: China Institute for the Control of Pharmaceutical and Biological Products) aqueous solutions. After 24 hours, the roots and leaves of the seedlings were separated, and the roots were washed with tap water and distilled water. Weigh 0.2-1.5g of fresh plant roots, cut them into pieces and place them in a glass tissue grinder, add 0.5-1.5ml of 10-100mM PBN in DMSO solution, and grind them evenly in an ice bath. Transfer the grinding solution to a centrifuge tube, warm it in a water bath at 20-45°C for 5-20 minutes, and then place the centrifuge tube in a refrigerated centrifuge (model: GL-8MS, purchased from: Shanghai Luxiangyi Centrifuge Instrument C...
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