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Callus culture method by taking Rhizoma Paridis roots and stems as explants

A technology of callus and culture method, which is applied in the field of callus culture, can solve the problems of lack of proliferation ability, easy fibrosis, and unsatisfactory results of prickly plants, and achieve low cost and fast growth speed , excellent texture effect

Inactive Publication Date: 2012-01-04
SICHUAN AGRI UNIV
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AI Technical Summary

Problems solved by technology

[0006] So far, the tissue culture research of Pachyphylla plants has not achieved satisfactory results, and only a few results have been reported.
The Journal of Sichuan Normal University (Natural Science Edition) Volume 29, No. 1 (January 2006) published "Induction and Cultivation of Callus of Plants of the genus Patricia" written by Li Qun et al. Callus induction culture was carried out on different parts as explants, and it was found that the roots and leaves of P. The formed callus is prone to fibrosis and does not have the ability to proliferate. In addition, they found that the rhizomes at the seedling stage are difficult to induce callus

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0017] (1) Selection of explants: select the rhizome of Prunus parviflorum in the seedling stage, cut off the first two segments of the rhizome with buds with a sharp knife, and make the wound small and smooth. The cut section is transplanted in humus soil, and when new roots grow out, the transplanted body is dug out and used as an explant for subsequent use;

[0018] (2) Disinfection of explants: wash the soil and dirt on the surface of the explants with running water, then put the cleaned explants into the Xiaoxiling solution and soak for 15 minutes, and then put the soaked explants Rinse with running water for 2 hours, take it out and dry it, transfer the dried explants to the ultra-clean workbench, first sterilize them twice with 0.1% mercuric chloride, each time for 6 minutes, and then rinse them with sterile water for 5 times , and then blot the moisture on it with sterilized filter paper;

[0019] (3) Induction culture of callus: cut off the buds at the front of the e...

Embodiment 2

[0023] Change the callus induction medium in step (3) of Example 1 to 1 / 2MS+6-BA1.0mg L -1 +IAA2.0mg·L -1 +NAA 0.5mg·L -1 + sucrose 30g·L -1 +Agar 6.5g·L -1 , other steps are the same as in Example 1, after 60 days of induction culture, the callus induction rate is 33.4%.

Embodiment 3

[0025] Change the callus proliferation medium in step (4) of Example 1 into MS+6-BA2.0mg L -1 +NAA0.3mg·L -1 + 30~50g·L sucrose -1 +Agar 6.0~7.0g·L -1 , other steps are the same as in Example 1, after 60 days of proliferation culture, the callus proliferation factor is 1.75 times.

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Abstract

The invention discloses a callus culture method by taking Rhizoma Paridis roots and stems as explants. The method provided by the invention takes specially pre-treated Rhizoma Paridis roots and stems as the explants; and the method finally realizes an effective induction and a rapid propagation on the Rhizoma Paridis plant calluses by inoculating the explants into a callus induction culture medium and a propagation culture medium to be cultured after disinfecting the explants. The method provided by the invention better solves the bottleneck problem of the traditional Rhizoma Paridis plant onthe aspect of tissue culture and lays a substantial theoretical foundation on carrying out all biotechnology researches on the Rhizoma Paridis plant in a deep-going way in future.

Description

technical field [0001] The present invention relates to a method for cultivating the tissue of the polyphylla, in particular to a method for culturing the callus using the rhizome of the polyphylla as an explant. Background technique [0002] Zhonglou is one of the precious Chinese medicinal materials. There are 24 kinds in the world, and 19 kinds in my country. Among them, the types and resources of the provinces in Southwest China are particularly rich, such as Yunnan, Sichuan, Guizhou and other places. The main active ingredient of Chonglou is steroidal saponins, and its aglycones are mainly diosgenin and pinanogenin of isospirostanols, and contain compounds such as amino acids, sterones, ecdysones, and flavonoid glycosides. Modern pharmacological research shows that Chonglou has anti-tumor, hemostasis, cough and asthma, antibacterial, antiviral and other effects, and its good curative effect has been used by many pharmaceutical industries. The main ingredient of importa...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 田梦良王跃华付伟李文光蒋亭亭杨军张珏张红玉陈燕王勇
Owner SICHUAN AGRI UNIV
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