Method for cultivating hybridized and backcrossed marine product fries

Abstract

The invention relates to a method for cultivating hybridized and backcrossed marine product fries. The method is characterized by comprising the following steps of: selecting excellent individuals that only a female area grows in a hybridized first filial generation family or group of Argopecten purpuratus and Argopecten irradians, and synchronously maturing the selected excellent individuals with excellent individuals of the Argopecten irradians; inducing the hybridized first filial generation individuals to spawn by using a conventional shade drying and temperature stimulation method so as to obtain unfertilized eggs; simultaneously, stimulating the Argopecten irradians to spawn and discharge seminal fluid, and filtering through 500-mesh bolting cloth to obtain the seminal fluid of the Argopecten irradians; and fertilizing the hybridized first filial generation eggs with the seminal fluid of the Argopecten irradians, cultivating according to a conventional method after hatching germ cells, and thus obtaining the hybridized and backcrossed marine product fries which are above 1.5 cm in shell height and used for commercial cultivation. The hybridized and backcrossed marine product fries cultivated by using the method provided by the invention simultaneously have the characteristics of the Argopecten irradians and the Argopecten purpuratus; compared with the Argopecten purpuratus, the hybridized and backcrossed marine product fries have obvious advantages on growth, individual size and temperature tolerance, therefore, the hybridized and backcrossed marine product fries can be cultivated in sea areas in the north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 50%.

Description

technical field [0001] The invention belongs to the breeding technology of shellfish aquaculture, and in particular relates to a method for cultivating sea hybrid backcross commercial seedlings. Background technique [0002] The bay scallop (Argopecten irradians irradians) is native to the Atlantic coast of the United States and was introduced to my country in 1982. The bay scallop grows fast, has a wide range of temperature adaptability, and has strong stress resistance. It is very suitable for farming in the northern seas of my country. At present, the output and farming area account for more than 50% of the scallop farming in my country. Due to the long-term inbreeding after the introduction, the germplasm of bay scallops has been severely degraded during the breeding process, showing that the size of commercial shellfish is getting smaller and smaller, and the mortality rate in the breeding process is high. For example, in 2007, the mortality rate of bay scallop broodsto...

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Problems solved by technology

[0004] Further studies have shown that although the first generation of hybrids can produce greater heterosis, there are still two major difficulties in large-scale promotion

First of all, because the temperature adaptation range of the imported purple scallop is very narrow, it cannot spend summer in Qingdao and the sea area south of it, and it cannot survive winter in the sea area north of Qingdao. difficulty

Secondly, since bay scallops and purple scallops are both hermaphroditic scallops and release sperm and eggs at the same time, the eggs are easily polluted by the same kind of sperm, and it is difficult to obtain a large number of unfertilized eggs for interspecific hybridization, resulting in the commercial seedlings obtained The proportion of true hybrid offspring is greatly reduced, which greatly limits the increase in yield

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