Method for cultivating hybridized and backcrossed Argopecten product fries

Abstract

The invention relates to a method for cultivating hybridized and backcrossed Argopecten product fries. The method is characterized by comprising the following steps of: selecting excellent individuals that only a female area grows in a hybridized first filial generation family or group of Argopecten irradians and Argopecten purpuratus; synchronously maturing the selected excellent individuals with excellent individuals of the Argopecten purpuratus; inducing the hybridized first filial generation individuals to ovulate by using a conventional shade drying and temperature stimulation method; simultaneously, stimulating the Argopecten purpuratus to spawn and discharge seminal fluid; filtering through 500-mesh bolting cloth to obtain the seminal fluid of the Argopecten purpuratus; fertilizing the seminal fluid of the Argopecten purpuratus with the hybridized first filial generation eggs; carrying out larva cultivation and fry intermediate cultivation according to a conventional method after hatching; and thus obtaining the hybridized and backcrossed Argopecten product fries having obvious heterosis. The hybridized and backcrossed marine Argopecten fries cultivated by using the method provided by the invention simultaneously have the characteristics of the Argopecten irradians and the Argopecten purpuratus; compared with the Argopecten purpuratus, the hybridized and backcrossed Argopecten product fries have obvious advantages on growth, individual size and temperature tolerance, therefore, the hybridized and backcrossed Argopecten product fries can be cultivated in sea areas in the north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 50%.

Description

technical field [0001] The invention belongs to the breeding technology of shellfish aquaculture, in particular to a method for cultivating commercial seedlings of purple hybrid. Background technique [0002] The bay scallop (Argopecten irradians irradians) is native to the Atlantic coast of the United States and was introduced to my country in 1982. The bay scallop grows fast, has a wide range of temperature adaptability, and has strong stress resistance. It is very suitable for farming in the northern seas of my country. At present, the output and farming area account for more than 50% of the scallop farming in my country. Due to the long-term inbreeding after the introduction, the germplasm of bay scallops has been severely degraded during the breeding process, showing that the size of commercial shellfish is getting smaller and smaller, and the mortality rate in the breeding process is high. For example, in 2007, the mortality rate of bay scallop broodstock reached about...

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Problems solved by technology

[0004] Further studies have shown that although the first generation of hybrids can produce greater heterosis, there are still two major difficulties in large-scale promotion

First of all, because the temperature adaptation range of the imported purple scallop is very narrow, it cannot spend summer in Qingdao and the sea area south of it, and it cannot survive winter in the sea area north of Qingdao. difficulty

Secondly, since bay scallops and purple scallops are both hermaphroditic scallops and release sperm and eggs at the same time, the eggs are easily polluted by the same kind of sperm, and it is difficult to obtain a large number of unfertilized eggs for interspecific hybridization, resulting in the commercial seedlings obtained The proportion of true hybrid offspring is greatly reduced, which greatly limits the increase in yield

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