Medicinal composition for directional controlled release of trace elements and preparation method and application

A technology of trace elements and compositions, applied in the direction of drug combinations, pharmaceutical formulations, active ingredients of fluorine compounds, etc., can solve the problems of non-specific disease site-specific concentration demand contradictions, constraints, lack of efficiency in element intake and utilization, etc.

Active Publication Date: 2012-01-04
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Any trace element must be subject to the "trace" requirements for the body. Although this type of metal element is very important to the body, the body content and intake are extremely low, which often leads to The body's uptake and utilization of such elements is inefficient, and it is prone to trace metal element poisoning
2. Contradictions between the non-specificity of routine intake and distribution in the body and the specific concentration requirements of disease sites
However, such methods are still subject to the functional status of specific organs. For example, if the function of related organs has been damaged, it is still difficult to achieve the therapeutic concentration and biophysiological effect of trace elements even through a large amount of dietary supplementation.

Method used

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  • Medicinal composition for directional controlled release of trace elements and preparation method and application
  • Medicinal composition for directional controlled release of trace elements and preparation method and application
  • Medicinal composition for directional controlled release of trace elements and preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Example 1 confirms that copper ions stimulate cell regeneration in vitro and stem cell mobilization experiments and the physiological effects of local film-controlled release of copper ions in the body

[0090] In vitro cell experiments have confirmed that trace element copper ions can effectively enhance the proliferation and differentiation of human umbilical vein endothelial cells (HUVEC). HUVECs were cultured and passaged in vitro. HUVEC in 5×10 3 Cells / well density were seeded in 96-well plates, and the cells were randomly divided into 3 groups according to the concentration of solutions added to the well plates, and the cells in group A were 5 μmol / L CuSO 4 , group B 25μmol / L CuSO 4 , group C is a blank control, each group has 4 duplicate wells, the basal medium is MCDB131, the cell proliferation is detected by MTT method and the growth curve is drawn. Take another HUVEC with 2×10 5 The cells / well density were seeded in 6-well plates, and the grouping was the ...

Embodiment 2

[0094] Example 2 confirms that copper ions stimulate cell regeneration and stem cell mobilization experiments in vitro and the physiological effects of local membrane-applied controlled release of copper ions in the body.

[0095] In order to determine the local effect of copper ions on the activation of scars and stimulate the regeneration of blood vessels in scars, the animal model of local myocardial infarction was pasted with copper ion gel to verify the physiological effect of local slow release of copper ions.

[0096] 1. Preparation of calcium alginate + CuSO 4 film. 10 ml of a 2% mass fraction of sodium alginate solution containing 10 mg of CuSO 4 The solution was mixed well, and 5ml CaCl was added dropwise 2 film after solution.

[0097] 2. Preparation of the myocardial infarction model: under anesthesia, the left anterior descending coronary artery of the rabbit heart was opened and ligated to prepare the rabbit left myocardial infarction model. In the state of t...

Embodiment 3

[0101] Example 3 Copper ion and albumin binding experiment (preparation of copper ion chelated albumin microbubbles)

[0102] The solution used in the experiment was prepared with deionized water, using 0.1mol L -1 NaCl maintains the ionic strength, uses Tris-HCl as the buffer solution, the pH value is 7.4, prepares HSA and copper sulfate solutions, the concentration is 8.76×10 -6 mol L·L-1 and 6.48×10 -4 mol L-1. Using a 1cm cuvette, measure (1) fix the excitation wavelength at 280nm, titrate HSA with copper sulfate, and observe the change of the fluorescence emission peak of HSA. It can be seen from the figure that Cu 2+ The addition of the copper sulfate did not significantly change the position of the excitation peak and the maximum fluorescence peak, but the fluorescence intensity weakened to varying degrees with the increase of the amount of copper sulfate. Since the volume of the HSA solution is much larger than the volume of the metal ion solution added dropwise, t...

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Abstract

The invention discloses a medicinal composition for the directional controlled release of trace elements and a preparation method and application. The medicinal composition is characterized by comprising hollow microspheres with the concentration of more than 1*10<6> per milliliters and the granularity of less than 10 micrometers, at least one trace elements which are 10 times less than or equal to the physiological dose of human bodies and pharmaceutically-acceptable carriers or minor ingredients, wherein the trace elements and the hollow microspheres exist in a dissociative form; and the hollow microspheres are prepared from pharmaceutically-acceptable film-forming materials. The medicinal composition is stable and safe, and can be guided trace element ions into the human bodies directionally under the action of ultrasonic irradiation to achieve the biological effect of the vascular tissue regeneration, tissue regeneration or tumor resistance of sites which are subjected to the ultrasonic irradiation effectively; therefore, the medicinal composition has a bright prospect.

Description

[0001] This application is a divisional application of a patent application with filing date: July 12, 2010, application number: 201010223282.3, and title of invention: a pharmaceutical composition for targeted release of trace elements, its preparation method and application. technical field [0002] The invention discloses a pharmaceutical composition for directional and controlled release of trace elements, in particular a pharmaceutical composition capable of directional and controlled release by ultrasound, capable of inducing tissue regeneration and anti-tumor. technical background [0003] Trace elements play a fundamental role in maintaining human health. The main physiological function is to play a catalytic role in various enzyme systems, play a role as an essential component or cofactor of hormones or vitamins, and form metalloproteins with special functions. The significance of the physiological effects of trace elements can be compared with that of vitamins, but ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/00A61K33/16A61K33/18A61K33/22A61K33/24A61K33/26A61K33/34A61K33/30A61K33/32A61K33/04A61K47/48A61K47/42A61K47/30A61K47/24A61K9/10A61K9/19A61P35/00A61P9/00A61P43/00A61P17/02
Inventor 周翔解慧琪康裕建
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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