Low-temperature ultrahigh-pressure preparation method of chlorella protein

A low-temperature, ultra-high pressure, chlorella technology, which is applied in the fields of biopharmaceutical engineering and the preparation of chlorella protein, a raw material for health food, can solve the problems of difficulty in extracting intracellular proteins, small processing capacity, low extraction rate, etc., and achieves a simple process. , short time, the effect of protecting biological activity

Inactive Publication Date: 2012-01-04
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, because Chlorella has a relatively complete cell wall structure, it is difficult to extract intracellular proteins, and generally requires wall-breaking treatment to effectively extract
At present, most of the traditional chlorella prot...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Mix chlorella powder and pure water at a mass ratio of 1:15 and stir for 60 minutes to obtain a mixed solution;

[0020] (2) The resulting mixed solution is sent to a low-temperature ultra-high pressure continuous flow cell crusher to extract chlorella protein,

[0021] Control the temperature at 5°C and the pressure at 180MPa, extract the collected solution as the solution to be extracted for the next extraction, repeat the extraction operation 3 times, each extraction time is 10min, and obtain the extract;

[0022] (3) The above crude extract was centrifuged at 4°C for 20min at a speed of 10,000 r / min, the supernatant obtained after separation was concentrated by ultrafiltration with a 30kDa ultrafiltration membrane, and the concentrated filtrate was freeze-dried at -20°C to obtain Chlorella Protein Powder.

Embodiment 2

[0024] (1) Mix chlorella powder and pure water at a mass ratio of 1:25 and stir for 70 minutes to obtain a mixed solution;

[0025] (2) Send the obtained mixed solution into a low-temperature ultra-high pressure continuous flow cell crusher to extract chlorella protein, control the temperature at 4°C, the pressure at 140MPa, and the extraction time for 30 minutes to obtain the extract;

[0026] (3) Centrifuge the above crude extract at 10,000 r / min at 4°C for 30 minutes, then concentrate the supernatant obtained by ultrafiltration with a 20kDa ultrafiltration membrane, and freeze-dry the concentrated filtrate at -20°C to obtain Chlorella Protein Powder.

Embodiment 3

[0028] (1) Mix chlorella powder and pure water at a mass ratio of 1:30 and stir for 90 minutes to obtain a mixed solution;

[0029] (2) The obtained mixed solution is sent to a low-temperature ultra-high pressure continuous flow cell crusher to extract chlorella protein, the temperature is controlled at 8°C, and the pressure is 150MPa. The collected solution is used as the solution to be extracted for the next extraction operation, and the extraction operation is repeated for 4 times, each extraction time is 25min, to obtain the crude extract;

[0030] (3) The above crude extract was centrifuged at 4°C for 20min at a speed of 12000 r / min, and the supernatant obtained after centrifugation was concentrated by ultrafiltration with a 40kDa ultrafiltration membrane, and the concentrated filtrate was freeze-dried at -20°C to obtain small Algae protein powder.

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Abstract

The invention provides a low-temperature ultrahigh-pressure preparation method of chlorella protein, which comprises: mixing chlorella power and pure water in a mass ratio of 1:10 to 1:30, and stirring for 30 to 90 minutes to obtain mixed solution; extracting chlorella protein from the mixed solution at 4 to 8 DEG C and under 100 to 200 MPa to obtain coarse extract solution; and subjecting the coarse extract solution to centrifugal separation, concentrating and drying supernate, and obtaining chlorella protein powder. In the invention, the application of the ultrahigh pressure makes the walls of the chlorella broken completely, the need of heating is obviated, and low-temperature operation is favorable for protecting the bioactivity of protein; the need of adding enzyme is obviated, and cost is expected to be reduced; and acid and alkali are not added, so pollution of acid and alkali is avoided. The method is simple in preparation process, quick and suitable for large-batch production, and is a safe and high-efficiency method for industrial preparation of chlorella protein.

Description

technical field [0001] The invention relates to the preparation of biopharmaceutical engineering and health food raw material chlorella protein, in particular to a low-temperature and ultrahigh-pressure preparation method of chlorella protein. Background technique [0002] Chlorella is a single-celled plant that can reproduce and grow rapidly. It is the only plant species on the earth that can increase by 4 times in 20 hours. Chlorella has high nutritional value and has the functions of inhibiting tumor, anti-cancer, enhancing immunity, anti-infection, detoxifying, protecting liver, and lowering blood pressure. The protein content of its dry powder is about 50% and the quality is also very good. Therefore, chlorella is a good protein source. [0003] However, because Chlorella has a relatively complete cell wall structure, it is difficult to extract intracellular proteins, and generally requires wall-breaking treatment to effectively extract. At present, most of the tradit...

Claims

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Application Information

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IPC IPC(8): C07K1/14
Inventor 张学武王晓琴
Owner SOUTH CHINA UNIV OF TECH
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