Low-temperature ultrahigh-pressure preparation method of chlorella protein

Abstract

The invention provides a low-temperature ultrahigh-pressure preparation method of chlorella protein, which comprises: mixing chlorella power and pure water in a mass ratio of 1:10 to 1:30, and stirring for 30 to 90 minutes to obtain mixed solution; extracting chlorella protein from the mixed solution at 4 to 8 DEG C and under 100 to 200 MPa to obtain coarse extract solution; and subjecting the coarse extract solution to centrifugal separation, concentrating and drying supernate, and obtaining chlorella protein powder. In the invention, the application of the ultrahigh pressure makes the walls of the chlorella broken completely, the need of heating is obviated, and low-temperature operation is favorable for protecting the bioactivity of protein; the need of adding enzyme is obviated, and cost is expected to be reduced; and acid and alkali are not added, so pollution of acid and alkali is avoided. The method is simple in preparation process, quick and suitable for large-batch production, and is a safe and high-efficiency method for industrial preparation of chlorella protein.

Description

technical field [0001] The invention relates to the preparation of biopharmaceutical engineering and health food raw material chlorella protein, in particular to a low-temperature and ultrahigh-pressure preparation method of chlorella protein. Background technique [0002] Chlorella is a single-celled plant that can reproduce and grow rapidly. It is the only plant species on the earth that can increase by 4 times in 20 hours. Chlorella has high nutritional value and has the functions of inhibiting tumor, anti-cancer, enhancing immunity, anti-infection, detoxifying, protecting liver, and lowering blood pressure. The protein content of its dry powder is about 50% and the quality is also very good. Therefore, chlorella is a good protein source. [0003] However, because Chlorella has a relatively complete cell wall structure, it is difficult to extract intracellular proteins, and generally requires wall-breaking treatment to effectively extract. At present, most of the tradit...

AI Technical Summary

Problems solved by technology

[0003] However, because Chlorella has a relatively complete cell wall structure, it is difficult to extract intracellular proteins, and generally requires wall-breaking treatment to effectively extract

At present, most of the traditional chlorella protein extraction methods still use repeated freezing and thawing, ultrasonic, enzymatic, acid and alkaline methods to extract and extract after breaking the wall. The extraction rate is low, the processing capacity is small, and the energy consumption is high.